hoooooly I just smashed that cover slip and wiped out the sample. I think I better go to sleep before I destroy the whole place.
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ha hhah now we’re cookin’! I got a quick writeup about microscope calibration. Now that I got it figured out, if anyone is attempting it…here are my tips!!!
Check the divisions on the calibration slide. Mine says 1DIV = 0.01mm, so then the division it’s talking about is the one between the dark lines. The lines can appear bright, or dark depending if you are using darkfield or not. Yah that got confusing heh heh. Now there are 1000μm in a millimeter so 0.01mm per div means 10μm per div. So then, I like to draw a line as long as I can over many divisions! for 4x objective I drew a line 100μm long, and at 40x I drew one 50μm long between the huge divisions not the smaller ones. Drawing a single line from one division to the next is very difficult and I fucked it up so many times before I got smart. It can skew the results lol! Final phase? Find someone’s SEM photos and compare their micrometer scale to yours. Chances are, if they are operating a SEM they have the calibration right rotfl! Once you have verified your measurements, it’s off to the races.
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shit, got to travel back in time and tell myself that…^-- 
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i want some of what your smoking lol
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I’m keeping that special shit to myself! I’ve been smoking pure trichome cores! yum!
Interesting that the core of the trichome is like 50% of the diameter of the trichome cap. You think the caps are 50% core by volume? They are cup shaped, so that reduces the volume some. I wonder what the dimensions of the cup are…like how deep is it? If I can get the dimensions of it vs the dimensions of the entire cap, then we’re cooking with gas.
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Interesting long filament structures radiating from the cores.
The bottom core in the last photo, is that partially attached to the white(ish) blob?
Cheers
G
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Yah there are some other trichome types in there as well. Probably stuck to a bulbous one. Could be remnants as well from other trichomes like the cap bits or something. I have no idea what some of the smaller things are there, like those filament looking things. I can see them at 40x as like a haze. I was thinking it could be the bits of cap still stuck to it then dried out? Guess I’d have to get out phase contrast to verify if that is the case. Not all cores have that filament structure on them, I focused on those ones as a curiosity. I’m sure they aren’t just image processing artifacts!
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You can see the filament ones vs the solid ones in this photo of the 90μm
The filament ones are light and you can almost see through them. The solid ones are dark orange and look substantial.
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I can’t find it now to show you but I found a picture of a lone grain of pollen pollinating a plant, well didn’t really find it, it popped up on Quora , taken by SEM
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so far, I can see this plant I examined was exposed to hostile conditions. That caused extreme trichome weathering. I wouldn’t have expected them to be so dinged up.
They’re plastered on them like face huggers on dry cleaners bags!!
My first though when watching the video clip was - maybe artifacts, but that photo clinches it.
So they are real… now, consider the lengths of the filaments you were seeing (first picture in post 345). They must extend through most of the volume of the trichome… 
(queue your favourite Arty Johnson clip) 
Cheers
G
Hey I got this way crazy video now.
Also still Photos of the pathogens that were moving so quickly.
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They remind me of that old movie I watched by ridley scott. Something about an alien?
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I think they are called rotifers. Anyone else got any ideas?
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Some kind of flagellated bacteria, I bought a cheap microscope to look at my living soil and never saw anything like that.
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nice find - looks like you caught some monotrichous bacteria. Flagellin monomers are vital parts of plant and animal immunity.
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I have to admit it was an amazing view I had to pick my jaw off the floor. When I saw shit living and moving in there I thought I was fucking hallucinating. Never thought it was that nuts. Little creatures living off algae colonies…woah!! Here’s the colony in a macro view.
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I thought I seen tardigrades in there as well. It was an eye-opener!
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