Microscope advise please
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Mine is an old school trinocular microscope with a digital camera. It’s for biological research and has 4,10, 20 and 100 objective. You can see more modern versions on a web site like amscope.
Here’s mine I have darkfield and light.
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Day 38 on the purple ice water! She’s bulking up fairly well, and hopefully most of the bulk is at the end. Looks like a good extract plant based on the trichomes. They have good sized caps already, and hardly any sessiles.
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Sex testing the bbl. I don’t think I got this one right, even though I see trichomes. Look at the core, are those leaf bracts? I know that long white thing looks like a pistil coming from the calyx, but I think it’s a unicellular trichome bent over there.
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Errrr I haven’t thought about what the yield is on this thing. I’m going with 1.5 percent. There’s too many sessile trichomes and gaps in the capitate layer.
Also! The bbl is female. I think I got them all right this time! We’ll see about the smaller seedlings.
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I really enjoy your threads @JoeCrowe 
Learn something new every time. And never know what you’re gonna say next.
Thanks for the knowledge and laughs 
Have a great one
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Thanks for the props! I’m working on this purple ice water and since I could smell something in the air, I thought I’d check it out.
I see a few oozing trichomes here! Ooo and a nice size on the diagram. I’m pumped! 170 micrometers and quite tall as well. A few golden looking trichomes. I’ll check the other plant, as well and in a few different zones before I call it trim time!!
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Here are some trichomes I smeared on the glass. Not sure why I did it, but there could be some kind of observation here. peers at the screen with rosin soaked pupils
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That would make a cool black light poster…
Cheers
G
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Maybe if you took some trichs from another cultivar or two (or three) to see if they “smeared” differently? Like, the way they smear might be some sort of predictor or whatever?
You never know… haha.
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Here’s some experimental rosin. What I have determined is, you can actually SEE the THCa crystals. Fewer crystals = less THC. Live extracts have way more THC than shelf dried. You can literally SEE it in microscopic images!
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Yah, looks like the purple ice water is done. See the trichomes where the cap is sideways? strings of resin? Trichomes that were once tall and proud are now crooked with age. Stalks terminate with thin fragile tips, 4 cells in diameter. Compare that shit to day 38 ish or whatever back there, you can see the trichomes are firm and tall. Young and growing still! Now, they are decrepit with age.
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hah hah watch this… I can make an observation from weeks ago 
using the purple ice water plants that I moved in there afterwards.
See. approximately 2 weeks ago, the trichomes were standing tall with no sideways caps.
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YES!
I’d snap up a handful of @JoeCrowe posters!
Monarch of the Trichomes!
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So what you’re saying is that for hash-making purposes, it’s better to harvest the plants early? As long as your main concern is total yields and not quality? Or do you think there’s a a corollary between the two? “Freshness” of trichome stalks/heads=better yields=better hash, as far as the actual high is concerned?
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A Live extract is definitely superior! If you leave the plant too long the color of the hash will be affected. The purple ice water is an experimental plant, so I’m using the trichomes to determine when it’s ready to harvest. They are in final stages! Of course, I think I got the stupid covid. hah hah probably got it just after new years, because I went to town on the 2nd.
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Here’s like week 3 or something. I don’t even know which day I put them in, lol! I can go check, of course. God-Tier productivity on this plant. Probably be shocking to most normal people!
The crazy phytologists published a paper on powdery mildew that matched with mine. I mean, all the mad ramblings I made about powdery mildew on the forums from my own phytology study has been backed up now by publications on the subject. They published just months after me. It was shocking, because I had thought there was at least a decade of lead time before they finished the same studies. hah! I’m only ahead by a handful of months. It’s really encouraging! They are catching up fast!!!
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HAPPY HAPPY JOY JOY! I got some biological samples from random plants. I’m supposed to put them on the yield spectrum! ooooo!! This is where I shine. I have the samples frozen right now, but I’ll trot them out tonight. Brands like cement shoes, and froot loops. some other shit. And something else or whatever, we’ll see what I have in the bag of wonders later!
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Would you elaborate more on this? Are you saying you can tell if a cultivar is a high yeilding plant by looking at with certain equipment? Maybe I am too stonwd for this right now 
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Oh you’re not too stoned! It was actually the reason I had come to OG again after so many years. Short story, long, I had to figure out where the hash yield was coming from, so I could make a future prediction. My thesis based on thousands of observations was the trichomes on the calyx, are responsible for the hash produced and so the number of them on a single calyx was an excellent predictor of the hash yield. I needed seeds, lots and random. OG is the answer.
I started out like a complete newb, knowing nothing. Which is the best place to be. A complete vacuum of knowledge, ready to be filled! So I started running plants, and taking photos. I would count the trichomes after harvest. OK fine I can count the trichomes after harvest and I’ll know if it’s good or not. Except, that’s useless. So, I take the process to the beginning of bud. Eventually I notice the plant has buds in veg. Then I notice the plant has buds when it reaches the 7th node of growth from seed.
Begin the new process. Taking photos of plants in veg and then running the trials. I compare the images. I have so many 2% ones. a few .5% ones. one single 3%. The data isn’t enough. After so many observations, my brain notices something. It’s mostly about the gap formed by the sessile trichomes.
Eventually I realize something. The hash extracts I’m making are composed of only capitate trichomes. The sessile I throw out. Things are starting to make sense.
So, when I say 3%, that means 3% of the calyx by weight is made from the caps of trichomes.
The trick is I have a series of images of plants that yield 1, 2 and 3%. I toss up the image of the new one and compare to the known ones. Whichever is the best match, is the future prediction.
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