First some backstory:
I was watching some real crime show, and a woman was murdered (poisoned) with colchicine. Turned out it was the girlfriend of the woman’s son.
Anyway, the defense tried to blame it on the woman’s husband because once upon a time he grew weed, and he apparently, according to the show, used colchicine to grow potent bud.
So I looked into it, and apparently it was used to make polyploids.
Anybody here ever tried it, or heard of it? What is the effect of making a cannabis plant polyploial?
I just picked up some Autumn Crocus seeds, which the seeds and plant are a source of colchicine, and was gonna experiment with it just for the hell of it.
Yes, its already mentioned in the marijuana Botany and I think first Experiments began in the 40s? Allthough I would have to Check sources again.
Increasing Chromosomes, normal Cannabis is Diploid. To get a triploid you produce a tetraploid with the use of cholchicine, cross that with a diploid and you got yourself a triploid.
Triploids Are worth nothing. They are Not Even sterile.
Hexaploids / Octaploids hold the Potential of outperforming diploids.
The effect is opening a field of new variations, without using an outcross to search solutions. It’s not really a one shot sequence, even if a “roulette style” is totally possible too. It’s not an innocent and magical process, it have a cost like all shortcuts.
Examples are a bit dumb with this process, it’s highly experimental and depend highly to the genetical context. But less say you have a line that just die if not under high RH, and that you’re searching at least a pheno that unlock this. By applying the right epigenetics factors on triploids iterations, you optimize your chances to reveal a “contradictory” pheno that will change the dynamic.
If you’re able to stabilize it, that’s the biggest part with triploids progeny beside herms ^^
The boosted potency is the common pattern of triploids, it don’t mean either that this potency will maintain past gen0 or that it’s 500% more strong. Just a bump.
You have to cross a diploid with a triploid to obtain sterility.
Well the study i found produced 8 seeds, of which 4 are viable, so not suited for commerical Production, maybe Thats Why Humboldt went with triploids instead of diploid x triploid.
But these 4 seeds would be infertile according to you?
And just to add…
The colchicine is systemic in the plant from the exposure of it to the seed
DO NOT smoke colchicine laced bud…
Use the seeds for breeding.
But dispose of any bud material from treated plants im pretty sure is toxic as fuck.
Cannabis is a diploid.
It has 2 sets of chromosomes.
Poly polyploid means it has more than 2 sets.
Take seeds from a diploid,treat them with colchicine.
Those plants should now be tetraploids, having 4 sets of chromosomes.
The colchicine treatment double it.
For the purpose…
I can give you examples.
Strawberries are octoploids today.
Meaning they have 8 sets of genes.
They were modified over the years to produce the large berries you see today.
By adding those extra chromosomes,it gave the plant the ability to create monster sized fruit compared to its “wild” or land race- heirloom genetics relatives.
Watermelons are triploids.
Youd get a triploid by breeding a diploid(2 sets) with a tetraploid (4sets) to get a triploid.
Even numbers of chromosomes will produce viable seed .
Odd numbers of chromosomes are sterile.
Thats how they make seedless watermelon.
The mac was a triploid.
As the science expends deeper into cannabis, instead of just size and sterility,id imagine theyre going to try to create super washers 10+% minimum…by increasing trichome density.
And every other Frankenstein thing you can think of.
Thanks for the literature and info.
I got a lot of reading up on it before I begin. Going in my plan is to basically experiment and see what happens. Im not thinking Im gonna re-invent the wheel or anything.
At this point all I have is soak the seeds in the solution, and grow them out. Was thinking of using s1s, and hoping to try to self them. And if that works, see if any off spring will sprout, and if any do, what the result is.
Any forseeable problems with that as a starting point?
Yeah, wasnt planning on smoking any of the first plants. I know they do use colchicine to treat gout, do prob safe in small doses, but wouldnt wanna inhale it.
Aren’t triploids effectively sterile though? From my understanding a triploid can only make viable seeds with other triploids.
I think the end goal of this is pretty clear. True agronomy-driven F1 hybrids, always autoflower, always triploid or higher. We would then be in the Monsanto world. Add in a few patents and boom.
The illudium i have right now wont take pollen from anything.Ive tried 8 different pollens not even a hollow white pip,Not one single seed.I have used various pollination times from a couple weeks as soon as a white hair popped up to waiting a good couple weeks then hitting again.Unless theres magic pollen out there this plant seems to be sterile as it gets
As long as the poly # matches up, theoretically can’t two 4-ploid breed, two 3-ploid breed, and so on? Or if not, then wouldn’t pollen from the same # ploid produce seeding effects even if not viable? Thanks for the info
I had a whorled irene from csi:h that was like this. When reversed she would make sterile pollen. When breeding with her, even csi:h said it was the lowest rates he ever had for success and he does open field lol
In meiosis, chromosomes pair up in homologous pairs (one from each parent) to divide evenly into gametes (pollen, eggs).
Even-numbered polyploids can split their chromosomes into balanced, equal sets — each gamete gets the right number.
Odd-numbered polyploids can’t pair their chromosomes evenly because there’s always an extra set.
California Indica, triploid gen0 … got showered sequentially by three males ^^ Nothing.
Abnormal floral development and shrinked ovulas … she’s 60ish atm.
Her sister, after obviously one month of continuous shower ^^ I finally got seeds at the last limit, crossing the fingers to can mature them fully right now. Plant is tired.
If you take your tired plants and put them in the sun,then clone the top of the new healthiest growth,and repeat the process throughout the summer of taking a clone from the last clone…and discarding the previous mother…
Youre basically running a tissue culture clean up
The emerging shoots grow faster than viroids and pathogens can move in the plant, essentially cleaning the cut.
Works fantastic.
