Well, let’s say the quiet part out loud: @bobgrows is an awesome grower who routinely pulls off cannabis coups! 
Bobbi!
That said, I was genuinely surprised that the injection method worked for her. There’s a lengthy OG thread on the subject and I thought the consensus was that injecting CS or STS just didn’t work. I’m watching her experiment with great interest.
Stil experimenting with fingers crossed…
Not to hijack, but I was wondering why you diluted the CS with water before injecting? Or did I misunderstand that part?
I diluted Silver Nitrate into distilled water to inject.
Yep, I found that injection thread and the quote from @slain about diluting the CS.
An easier method for reversing using straight silver nitrate - #502 by SargentPiff
Eyes On your experiment Bobbi, best wishes.
-Grouchy 
Thank you @GrouchyOldMan
Hoping it works!
Not sure where you got that idea 
It worked fine for me and several others that posted in that thread 
Gen Six IBL Seeds & Bud
Here’s a glimpse of the very first of Frankenstein’s Daughters 6th generation seeds peeking out of their pods!
The IBL-seed plant is tiny and I’ll probably only see a handful of magic beans, but given the history of this improv grow I’m pleased to see any at all. The paper plate is insurance in case any seeds drop early. Can’t afford to lose a single one!
I harvested the IBL-bud plant today after seeing amber developing on the calyx trichomes. This is the end of week 8 of flowering, pretty typical for Frankie. However, unlike the original Frankenstein clone-only cuts, the pistils on this plant all turned red as harvest neared.
The Frankie Daughter BX-Bud plant is a week or so behind and she is still throwing white pistils like her “Great Grandma” Frankie S1. These BX plants also seem to have a strong tendency for purple coloration as well.
So, the IBL(inline breeding) & BX(backcrossed) lines reacted very differently on waking up from hibernation. Is this a phenotype display, or just variability due to the conditions the two plants experienced? We’ll find out soon.
The BX pollen plant finally produced pollen but it was too late to use on this run so I dried and stored it in the freezer for later use.
Felicidades y Prospero Ano Nuevo Amigos!
-Grouchy
[Edit: clarified a phenotype hypothesis]
Very cool findings 
I’m at a loss to explain it though…
Cheers
G
I am no expert in any way on this subject but I was recently reading an article that claimed that male cannabis plants that are to “masculine” are much lower producers of pollen. I don’t know the validity of the information but makes for an interesting read:
Thanks for that comment @Oldjoints. The article is very interesting but it seems that the takeaway is that reversed plants generally produce less pollen than natural males. The design of the study doesn’t correspond to the reversal protocol most of us use in that they wait till pretty late in flowering (weeks 7-8) to begin the chemical reversal, then dose Colloidal Silver daily (and heavily!) for two weeks.
> The randomly selected 12 female plants underwent daily chemical treatment for two weeks (week 7 and 8 of the flowering growth period). Chemical treatment consisted of 15 mL of colloidal silver (Trace Minerals Research L.C., Ogden, UT, USA) diluted to 20 ppm concentration in filtered water, which we vertically sprayed onto the plants, once daily, for 14 days [6,57]. After chemical treatment, all 12 female plants developed male inflorescences and began male flowering during week 9.
I have been starting treatment in the first week or two of flower and only spraying periodically rather than daily as in the study protocol.
So, that’s food for thought, but my suspicion is that I’ve been overdoing the application of STS on my reversals.
Looking back, my best pollen harvests were when I started this breeding project and I followed the “consensus” on CS and STS application: Once every 4-5 days until male anthers appeared. Lately I’ve increased the reversal routine to once every three days until I see the boyz balls appear. The increased frequency did not shorten the time until male flowers appeared, and seems to have reduced the amount of pollen produced.
The next run I’ll try to do a side-by-side reversal with two fem clones, one reversed per normal, and the other later in flower, with less STS over a shorter period of time.
Appreciate the input and interested if anyone has anything else to add.
-Grouchy
I took it that they were talking a lot about using hermaphrodites in this process (co-sexuality).
But they also talk about reversals.
There methods had me a bit confused as well as their terminology.
Harvest Time
The IBL & BX bud plants finally ripened and at the first sign of amber trichs on the calyxes I chopped, hung, dried and am curing about five ounces of fresh bud.
IBL & BX at their day of reckoning
Small, sugary and potent buds and a pile of gold dust kief. I’m busy making QWET proofing tinctures to sample the chems.
I also dusted the remaining BX-seed plant with some of the BX pollen tho I doubt it is viable. Moved this one to the grow room, we’ll see what happens.
So it goes,
-Grouchy
.
Fingers crossed. Good luck @GrouchyOldMan
BTW, @SaintAliasKnife has a sweet Frankie’s Daughter BX5 all trained up and ready to stretch out her limbs.
The Spice must grow. Blessed be the Little Maker! - #865 by SaintAliasKnife
She looks like a twin to mine, heterogeneity in structure and leaves. Here’s one of my BX5 plant from back in November with the same pruning.
After long reflection, I’m attributing the purpling on my BX plants to a strong reaction to my overly aggressive ramping up on the LED lights as they awoke from hibernation. In essence they were sunburned to a crisp.
The IBL plant blushed a bit of vermillion too, but it was couple weeks younger, had its own lamp and a much slower PPFD ramp.
That’s my guess and we’ll soon see as the next batch will have a more normal grow cycle.
-Grouchy
Frankenstein’s Daughter IBL Seed Harvest
Today I started plucking the first of the ripe 6th generation seeds to poke their heads into the light.
I captured about a dozen so far, but there are a bunch more fat seed pods on this little plant. More than enough for germination test and a serious seed run if I like the look of the plants.
These seeds are from the Inline series, created from two BX5 seedlings, one of which was reversed with STS for pollination of the female plant.
It’s not clear yet if we’ll see any seeds from the Backcrossed (BX6) line. Those were created from a single BX5 plant that I cloned and reversed to pollinate the female. No sign yet of any seed-bearing bracts. If the IBL plants look good, I may not revisit that BX thread.
So, that’s the news from Grouch Central, come March I’ll have three years into this project. Whew!
-Grouchy
Nice, time sure flies.
Another one in the books.
Great work @GrouchyOldMan
I want my Mommy!
I’m collecting seeds daily from the FD IBL-Seed female, a couple dozen so far. It has become a welcome task each morning to put on the magnifying specs and go searching for newly opened seed pods. Once discovered, I have been gently teasing the new seeds free with a set of hemostat pliers. I have been finding three or four every day. Only one immature dud so far. Eventually, I’ll chop the plant and squeeze out any late bloomer seeds.
And that is why we’re talkin about Mothers now. Specifically Bonsai Mothers.
If these 6th generation feminized seeds germinate well and grow the nicely structured heterozygous plants I expect, with the chems I’ve been testing, we’ll want a healthy Mom plant on which to base the Frankenstein’s Daughters IBL seedline. I hope that doesn’t sound too pretentious, but that has been my goal since I ventured down this path almost three years ago.
So, I think I’ve got this strain where I want it and sprouting a few of those seeds and picking a Mom has gotta be the near term goal. From that Mom will come cuts and a serious seed run.
Cheers, and thanks for following along,
-Grouchy
PS, the latest “Proofing” tinctures have been sublime! 
