Moving onto culture procurement.
I labeled my deli style containers and left the test tubes alone for this attempt. I wanted to try a couple different things before going with the test tubes.
I started by taking a regular size clone and rinsing it for about 5 minutes in distilled water, then into a 2%bleach solution for 10-15 minutes. Finally back into distilled water to rinse.
I keep all my tools in a solution of 10% bleach to keep sanitized.
First container: Banana Breeze #1 and went with 4 cultures.
Second container: Banana Breeze #2 and I decided to go with 8 cultures, 4 being not what I would instinctively use. Stem nodes with no leaf.
Third container: Banana Breeze #3.
Fourth is Pacific Pie #1.
Fifth is Pacific Pie #2. I did 8 for the hell of it.
Spraying a 10% bleach solution on hands often and only opening containers for an extremely short period while inserting culture will help mitigate pathogens/microbes contaminating your media and cultures. If a plant has pathogens inside of the plant it will culture as well making it hard to determine the culprit.
This is stage one of a multi stage process so please bear with me. I will make a new batch of solution with a different mix of auxins/pgrs. I hope to be open and share it all! ![]()
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