Thanks for the reply, that’s what I was originally thinking as well. Bodhi seeds described the outcrossing of the pipeline male he used for the v2. “…… F1 hybrids with the line are showing a 50% blood traits. Blood
Expressing hybrids of hybrids are
Showing 0% blood traits with a limited experiment of only 6 seeds.”
In my run of 8 plants I selected the two with the most solid ring that were female. Out of the 8 I had 3 males and 5 females. Two males displayed a bleeding traits but not as dominant, and then I had a total of 3 females that bled. That’s 5 out of 8 plants that bled. Then selecting females and reversing should narrow down the gene pool. I also got S1s from the reversal. If the gene pool needs to be narrowed more, should I back cross to the S1 or further inbred and later cross back to the S1 say an F3 or F4?