How do you create multiple varieties of seedstock each season, and know to the best of your ability that the varieties are “pure”? How do you contain and isolate each variety’s pollen so as to not cross contaminate?
Is this done in separate rooms as indo?
How do you do that outdoors??? just grow a male nearby to females? How do you keep multiple outdoor varieties from crossing unintentionally? Just grow diff varieties far away from each other? Only grow one male variety?
Would saving and storing pollen, and then later applying it to certain specified females branches help the outdoor issues??
I’m just trying to wrap my head around how to make multiple lines of stock, whilst isolating breeding projects. Thank you!
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I can’t say for outdoor.
For indoor I grow my males alongside my females until they are about to pop then I remove the males to a separate room with no moving air, put them sideways over newspaper and collect that way.
I’ve been successful with 2 different types of pollen at the same time but I’m not sure I’d want to try 3.
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I would never want to attempt this at scale, but last summer I pollinated a number of lower branches on one plant outdoors using pollen I had stored in the freezer that I applied with a paint brush. Despite having a couple other plants within six feet of the pollinated plant, no contamination. I applied the pollen, waited a bit, and I sprayed down the pollinated plant to kill any lingering pollen.
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What spray do you use to kill pollen? Just a water spray?
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I’m currently running 2 seed chucks in 2 tents in 2 rooms 2 floors apart. One has 18 strains, the other 15.
18x
15x
In both cases I used clones from my mothers, and then when it was time to pollinate I dusted the tent (fans on) with the pollen collected from another run.
Each tent has air venting outside so any pollen in the air ends up leaving the house instead of contaminating the other tent.
Cover a lower branch with a large paper bag that has a flap cut in it (3 cuts of a square, with the 4th part still attached to the bag so you can lift it up like a trap-door).
Now tape up the bag to the branch and on a windless day use a paintbrush and pollen to apply the pollen to the isolated branch in the bag. Once done immediately tape up the flap and leave for a few days.
Paper bags breathe so you have several days (unless it rains) to leave it for the magic to happen.
I like to look at the pistils through the flap to see if the pollen took. Then before I remove the bag I mist water into the opening of the bag to saturate any left over pollen so removing the bag doesn’t share with the rest of the plant.
Also mark this branch with a loose quick tie so you can identify it as the seeded branch later.
Theoretically if you bagged a bunch of lowers and then systematically pollinated each one separately and apply a decent amount of OCD you could pull off a multi strain run but there is always (with outdoors) the concern for errant pollen from elsewhere.
This is how you do it. 
You got this bud, keep reading up here on OG and check out all the co-op seed runs and you’ll gain that XP fast @ThunderDump !
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There are a few methods that are effective, but it depends on your objectives. Producing a thousand seeds requires a more aggressive form of pollination.
Producing 200-300 seeds can be done very easily.
One of the most effective ways to collect pollen, from multiple males, is to keep them small. Inspect them daily and pluck the flowers that are close to releasing pollen. I place those flowers in a black film canister. 2 days later, they will release the pollen in the canister. This method will prevent any form of cross contamination.
When applying the pollen, I take the female to a separate room and brush the pollen onto 1-3 of the lower buds. Twist tie a sandwich bag around the pollinated buds and leave it on there for 2-3 days. The humidity will destroy all the extra pollen that wasn’t used.
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Ok that’s epic, I’ll try that in the future. How long does the pollen last in the canister?
Depends on how you store it. I usually leave it in a cool dry cupboard. Pollen will be viable for a few weeks.
You could try freezing it. I dont have any experience with long term storage for pollen, but there is plenty of info on how to do that.
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