Any Tissue Culture Gurus in the house?
Been doing fungal tissue culture work for a while now, growing mushrooms, looking to add plant tissue culture to my repertoire. Just starting with the basics, need to do some cloning and mother storage, then on to old seed rescue, and after that need to sort out meristem culture to eliminate potential viroids.
Anyone here doing that stuff?
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Seems I recall one of the mods being somewhat interested. Do a search here for “Tissue Culture”
You should get several interesting hits. This kinda stuff intrigues me. Ill follow along.
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I have done a few searches, I understand the basic techniques, and am confident in my sterile technique, mainly I’m hoping to find someone with experience in the various media recipes, and the variability in recipes between strains. And how to know which plants and teks need what.
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This chick has TONS of good info. Not specifically cannabis, but useful none the less. Plus she’s cute as hell, so an added bonus.
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Thanks that and plant cell technologies channels are where I have learned what I have so far.
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I’m hoping @Chimera will chime in, if I recall he was talking about this stuff way back in the og, og days. He obviously knew what was up even back then, when it all sounded like magic and hocus pocus.
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My god, smart, nerdy, funny, loves plants…
I so badly want to learn tissue culture, but I fear I may fall in love with a younger woman in the process 
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I suspect tissue culture won’t be that difficult, the general principals aren’t that much more difficult than mushroom tissue culture, the primary difference seems to be all of the various mediums, I only need a handful of media ingredients for most fungal cultures and procedures, but almost every procedure with plant tissue culture seems to need a different media, and many of the plant growth regulators, are destroyed or weakened by the sterilization process. So that adds a layer of complication, plus with mushrooms you don’t need to sterilize the outside of the mushroom before taking a tissue sample, you just pull clean tissue from inside, with plants it seems exactly what portion you culture, and the media you put it on, is as critical as the proficiency of your sterile technique.
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If you stumble upon or create your own cannabis protocol for tissue culture, PLEASE do share it with us. Even the girl @ Plants in Jars breezes over this topic but never directly provides a working protocol for cannabis
Honestly? It seems like you’re our guy for this 
but I do hope someone else chimes in here
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I would check this thread.
@SCJedi or @Marcus420 may be able to provide some insight in regarding protocols or some white papers to read.
A lot of this is going to be proprietary information though. I don’t think anyone is going to give you their SOP without an even exchange for said information. Maybe try the book that comes with this kit:
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This is a good listen
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Don’t tempt me lol. I already do a mushroom breeding podcast.
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One week from tomorrow, Saturday December 14th at 10pm EST, a buddy of mine at Great Lakes Tissue Culture will join Jay Blaze on the Organically Blunt podcast to talk about both cannabis tissue culture and ebriotic (seed) rescue. Hopefully this will include some in lab demonstrations. If you join in live you could have some of your questions addressed via the chat.
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I believe she (PlantsInJars) and others like her intentionally avoid the word cannabis to keep from being demonetized by platforms like YouTube. Some there will use other terms though, like spicy tomatoes, while specifically working with cannabis. It is an odd game …
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Please share! I’m always looking for new podcasts.
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I think more specifically, she just doesn’t want any undue association with assisting anyone grow “drug cultivars” 
and I don’t blame her. Her YouTube channel is basically part of her live resume, if you will.
BRO! Can you ask your buddy to specifically talk about tissue culture protocols for cannabis roots & shoots?? Would be INSANELY helpful
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Yeah, she gives pretty good info on the basics, and how to DIY a few things.
Ive been loooking into it for a while trying to figure out how to rescue some old seeds I have.
There are some papers on the correct culure medium protocol for cannabis out there. Pretty sure alot of the Amazon kits just use sugar water, which is just a general, can work in alot of cases medium. But often auxins and cytokinins in specific ratios are needed to activate growth.
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If you’re interested in some reading material, give this a look. Dropbox
It’s kind of a hodge-podge of info, but there’s some good stuff if you’re just starting out.
The Hemp Kit doc came with the starter kit I bought a few years ago. There’s a textbook about TC experiments that’s real good. It’s not canna specific, but has some good basic info. There’s one about TDZ Direct Shoot Organogenesis that I used for the media formulations, lighting, temps and such.
Botany and Biotechnology of Cannabis Sativa L is good too, but it’s got some hardcore science that goes over ny head.
Anyway, I hope you find something useful. 
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Nice collection of TC books. I checked my folder and have three in addition to those. Guess they are more just scientific papers, but could still be useful
(plant preservative mixture NOT parts per million )
Not sure if this is going to work, but clicked and dragged. Looks like it did!
Use of PPM To Control Contaminants.pdf (196.5 KB)
PPM General Usage Info.pdf (83.2 KB)
Ppm & Shoot Regen.pdf (419.8 KB)
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