Selfing, Backcrossing & Inbred Lines

Greetings @setgoals,

I spent the last year on a breeding project similar in scope that was intended to create a “Feminized Seed” version of a popular “clone only” strain.

In the process, I reversed the original clone for pollen then pollinated a sister clone for S1 seeds. Those seeds were grown out and then backcrossed with pollen from the original clone. The next step will be to grow out the back crossed seeds and see if they are closer to the mother clone.

If you go the S1 path be aware that your S1 seeds may not be very homozygous, producing a variety of very different phenotypes leaving you to decide which ones to move forward with.

If you are interested in more details on this approach, here’s a link.

Frankie’s Daughters: Unpacking a Frozen Genome

Best wishes on your project,
-Grouchy :v: :green_heart:

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