Synergy Genetics - Tissue Culture Q & A

Many thanks SC_Jedi for the post and info!!!

I really would like to take this opportunity to ask you few questions and maybe enter into the topic.

  • Benzlaminopurine (BAP) I Bought it…is it needed?
  • PPM I didn’t buy as it is very expensive ( BAP as well). I read some protocols are not using PPM.
  • Protocols: any link to free sources? Protocols specific for different type of explants. leaves is more difficult that stems? Could be root?
  • Tricky one: what’s your opinion about mushroom incubator ( which simulate a laminar flow but without the HEPA filter, etc,etc,etc,etc) I like Plants in Jars YTVideos. + Kitchen well clean + pressur cooker as autoclave or microwave as steriliser.
    Which % of success would you believe I could achieve?
  • Timing. After success can you control the grow to reduce or accelerate the passage to acclimation? Any link about timing from a leaf to be a 5’ cut. How long does it take? Can you use tissue culture and fridge to store genetics for long time ( 1-2 years before need to change medium)?
  • DIY/Homemade: any link/info appreciated.

Many thanks!!!

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