Tonygreens RIL Group Buy Grow Show (Closed)

[Hugh_Glass]

@Daytripr69

recombinant inbred line

I for one had no clue what that really meant. Pretty intriguing if you read up on it.

Recombinant inbred strain

[Kingmambo]

I’m in for at least one pack.

[Howard.Crane]

More info in this thread:

Gorilla Glue 4 Recombinant Inbred Line

Hey to old friends and how do to new ones! Pull up a chair and invite a friend because the GG4 RIL seedrun is about to begin! I have been working, documenting and line breeding the Gorilla Bubble for 6 or 7 years. The end goal is to create a pure homozygous line to make supreme F1’s. As some may know we have been very successful proofing and checking our work a long the way, with many exceptional hybrids grown out by people all over the world. Tom Hill suggested this scheme to me but before we…

[Vagabond_Windy]

From what I can remember Tony’s been at ICMag forever and was really close with Josey and crew.
He’s worked on Gorilla Bubble for at least 5 years possibly more. I believe the RIL might be using his and Mycoteks work on the GG4 or maybe I’m just tweaking.
Either way, his work is awesome and speaks for itself. This is kind of a huge deal

[Vagabond_Windy]

This @Badger

[Howard.Crane]

@Vagabond_Windy

From what I can remember Tony’s been at ICMag forever and was really close with Josey and crew.
He’s worked on Gorilla Bubble for at least 5 years possibly more. I believe the RIL might be using his and Mycoteks work on the GG4 […]

Sounds right. I remember being really intrigued by Gorilla Bubble on icmag a long time ago, lol. Been following Tony ever since.

@Tonygreen

much work had to go into Gorilla Bubble to create a GG4 near isometric line. Fortunately Mycotek put in similar effort with his chem outcross based GG4 NIL. Both lines together make this scheme possible, roughly a combined 12 years of inbreeding work!

[Vagabond_Windy]

Same. I had an itch for Gg4 after smoking some in 2015 and I found his Gorilla Bubble.

[Badger]

Thanks for info guys!!!

[Purple-N-Hairy]

Still not sure if this is for the GG4 or the GB. @Tonygreen or @Hugh_Glass, can you clarify?

Either way, I’m in for one pack. If both strains are available, I’m in for one OF EACH!

[Hugh_Glass]

GG#4 ril …

[mtntrogger]

Most likely gonna get in on this one. Tony is the man ! Been following his work for several years. Lots of dudes on IC have run his stuff over the years. Gotta support G’s like him

[Tonygreen]

To make the Recombinant inbred line we use 2 separate inbred backcross lines derived from the same recurring p1, in this case Gorilla Bubble bx5 is used with mycoteks bx6. Both lines have well over 95% gg4 recurring DNA, when they recombine they create hybrid vigor while maintaining the gg4 genome with many homozygous gene pairs.

Even though they have been inbred to contain mostly the same DNA hybrid vigor is induced without creating massive heterozygosity like in most unrelated f1.

[Ginger_Rick]

Just gotta say thanks again for all your great contributions to the community! Lots of cats drop nice beans, but few are the ones who put the real work and craftsmanship in that this plant truly deserves. Even fewer still are the ones who bring that craftsmanship and drop the science for all to share. Really great work man, we are all in your debt. Way to do the plant proud.

[Indoornesian]

I’m absolutely % N.
I’ll be here tagging along for tha show too.

[Tonygreen]

The technique can also be used in full sib breeding to reintroduce vigor after stabilizing, in that case you would breed down separate full sib lines then recombine later. Maybe an easier example to conceptualize.

[lefthandseeds]

@Tonygreen

The technique can also be used in full sib breeding to reintroduce vigor after stabilizing, in that case you would breed down separate full sib lines then recombine later.

I’ve thought about trying this quite a bit recently, especially for helping to improve selection in recessive traits. For example if you were breeding an auto, you could isolate the auto gene in an F2 male and cross that back to a female of your F1 generation. The generation resulting from a F1 x F2 cross will be 50% autos, instead of 25%, which is a big help if you’re a hobbyist growing 5-10 females.

[khaoohs]

How would you know you have isolated the auto gene in the male F2? By pollinating sisters and if any of the F3 gen are auto?

[lefthandseeds]

I’ve never bred or even grown autos, but I’d think you just keep it under veg lighting and keep the ones that start to flower. Should happen the same way as an auto female I’d think.

[Tejas]

Count me in, I’ll support the project!

[khaoohs]

Thanks, wasn’t even thinking that way, got it now.