Understanding breeding, how to achieve the best an strongest high, false beliefs an inbreeding depresion

@HolyAngel I would think so, the probability is high for autonome fission. Its how dna change size over time. Its hard to find if you don’t have plants to compare to or if you don’t have decoded the dna. What seems to be abit of a problem with cannabis is that the dna is more diverse then say, humans. But with time we will be able to decode the full dna range of cannabis. I’ve seen many papers of decoded cannabis dna, but its always within a certain cultivar.

@Pannonian Well we do know more now then before, but we are far from a complete picture. I think we are quite close to understand what does cannabis thc potent and we do have lots of very thc potent weed around. Problem i have with the current climate is that we don’t understand the full effect of cannabis. We have starred us blind on THC levels which have reduces all the other compounds which actually makes you super duper high. The plants I’ve tested that have crushed my brain have never been over 22% thc and the home test I’ve had have never been able to show anything else then thc and CBD levels. Same with the common terps, from the test sheets it’s always been ordinary plants but when everyone smokes it, they go bonkers! I believe that it’s a combination of molecules that drive a perception of potency and not the thc. Thc only opens up the brain to be sensitive to this combination.

Pz :v:t2:

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Well when breeding Cannabis Indica var Indica to a Cannabis Indica var Afghanica do we usually breed the male Indica/Indica male to the Indica/Afghanica? If the female is the afghan then does she exert her mitochondria on the rest of the genome? I wonder if the mitochondria is tied to the roots and what do the sterols play. The flavainoids exert their influence and don’t have any chlorophyll

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plant tissues devoid of chlorophyll, and thereby unable to photosynthesise, could support the biosynthesis of cannabinoids within their own glandular trichomes. The carbon source required for cannabinoid biosynthesis would have been produced elsewhere within the plant and then translocated to these trichomes. This demonstrates that in a normal growing environment, where some parts of the plant are in full sun and others in varying degrees of shade, all aerial parts the plant will be able to synthesise cannabinoids. The resultant increase in trichome content uniformity is fortunate for the grower of cannabis.(Potter D. 2009)

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A female will copy mDNA and chloroplasts to the zygote(1 cell embryo) inherited from the egg cell during mitosis and meiosis. mDNA is isolated in the mitochondria and is never a part of the dna spiral.

Pz :v:t2:

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Zy-:ram: my favourite goat.

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Thus the mitochondria is only specific to the female gamet and does continually picking an afghan female affect the effects of the cannabinoids and such? The mitochondria just goes along with the cell from the female so…

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@Cactus Yes, pollen does not contain mDNA. All her children will have the same mDNA, and their children :grin:

Yeah you get it.

Pz :v:t2:

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Now when you reverse a female to produce pollen (donor) it does not contain mDNA only the female flower that is receiving the pollen would have the mitochondria?

Latin proverb: The poison is in the tale (tail :wink:)

The reason for the tail comment is that when the sperm cell attaches to form the zygote it is cleaved off or not incorporated into the female cell. Thus only the mitochondria from the female remains. Not sure what the mechanism for pollen would be since their is no reason for a tail?? So… Mind you this is just spit-balling ideas from others and such. No hard fast rules just ideas :upside_down_face:

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Yes, you are correct. The mDNA are passed from the mother in the initial steps of making a seed.

Pz :v:t2:

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Oh nice, Tom’s around…think ill start checking OG again now…

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Brooo, where you been ?!

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Well there was this blow up about hay vs. straw and i just didnt like the vibe being thrown my way by some. So went back to old stomping grounds. Hey mithri :stuck_out_tongue:

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I’m a little late to this discussion but I have frequently used hermi polled for making seeds without ending up with more hermi plants.

Hermi pollen makes females not more hermies has been my experience.
I had noticed this occurring and then I found a research paper that backs it up.

So far I have tried this about 5 times, all with the same results.

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What we are really talking about is autosomal modifiers acting upon sex chromosomes. It totally happens an XX plant can fully appear XY in the extreme. But it is highly biometrical genetics or quantitative trait loci inheritance if you will. Epistasis to the max, many digits need to fall into place for it to even happen. That’s why you don’t see it breeding true in the following generations. That’s why it’s not the problem folk try to make it out to be.

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That’s really what we are talking about. Mostly homozygous individuals vs mostly heterozygous individuals. The importance is highly intensified in the realm of drug type cannabis, that’s all. It ain’t maters or corn etc. It need make you feel a certain way when smoked. That’s a maths clusterfuck compared to anything else in the plant world.

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Do they care about the cocoa plant this intensely or just the intense high when snorted

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True…and why the environment has such a huge input to the expression of any phenotype, so that what you thought was stable in your environment comes out looking and behaving in a completely different way in another part of the world. The environment causing differences in gene regulatory proteins and trait expression is a whole other rabbit hole!

Personally I’ve stopped even attempting trying to select for total uniformity, for me the cons outweigh the pros… I’d just a sooner start with more plants begin with and then select for morphological traits that I have seen frequently in combinations with later flowering traits that I want. It’s not exact, but without variation there is nothing new to look for!

Inbreeding a strain to within an inch of its existence is solid goal if reliable F1’s are what you are looking for but man it’s a huge effort that can easily be a dead end too🤷‍♂️

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I think it’s important to also note that environment has a variable effect on genotype. Yeah the maths just get worse man :joy:. Like ok, genotype 672, let’s call it for accuracy sake sour d, is greatly affected by environment while genotype 974 let’s call that blue dream - delivers the same phenotype in a far broader range of environment.

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F1’s let’s calll them heterozygous. This is the opposite of inbreeding depression mostly because it begets 4 different genotypes, providing higher adaptability to the tune of 4:1

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And interestingly, the greater the genetic differences between two inbred parents, the greater the hybrid vigour of the F1, if this is due to repressing deleterious pairs, enhancing beneficial pairs or something else is still an area of debate and research. In practical and general terms; an F1 between say an inbred 100% Sativa and an inbred 100% indica will result in hybrids with the most hybrid vigour. This has been my experience of it, but there is so much we don’t know or understand so there is still some ‘art’ to it lol.

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Go to the next generation, a segregating generation. Transgressive segregation appears in that same higher ratio. And that is far more important than heterosis / hybrid vigor when it come to drug type cannabis.

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