Another version of the ubc MacGyver. Whats interesting is they all seem to glaze over the power supply… (Hackteria used food coloring btw as their stain)
But here’s the question… How do you get intelligible data from
Hmm yeah, that was my thought when I saw the plate also… small img though so it might be deceptive… if the bands are at least semi consistent, then it should be possible to compare gels side by side… After having a look on eBay, gel electrophoresis equipment can be got for peanuts… it’s consumables that are a pita.
So let’s be more practical… So ladder is on top, then your individual channels (with same material different reagents) give you your AGCT results on the base pair scale? Am i understanding that about right?
Yeah, that would be my understanding ofthis method to determine the existence of the alleles in the expected location, though you would expect that ANY drug type strain this info is moot… i.e, from what I have been able to glean from further reading, it’s the number mRNA transcripts that determine to what extent the trait is expressed. Which would seem to me the data you really want to get at.
There is a paper on it here…
Inflorescences of marijuana‐type and hemp‐type plants yielded significantly different quantities of cannabinoid synthase RNA transcripts as measured by RT‐qPCR (Table 2). Expression of THCA synthase in marijuana inflorescences was 102‐fold greater than in hemp inflorescences (P < 0.05, t‐test), whereas CBDA synthase expression in hemp inflorescences was 113‐fold greater than in marijuana inflorescences.
What is not entirely clear to me yet, is if/how these transcripts are analysed/visualised… keep reading I guess
