PROTOCOL 0 "clean your plants"

I can see your point…no pun intended! The thing that would bother me would be weak genetics like clones off of clones of clones so in the end you got a clone 12 times removed from the seed mother. Which would be a weak plant and if you have invested a lot of time and effort into your grow why chance it right? Plus if you were that deep into you wouldn’t need that one clone you’d be bringing in 500 of them. I guess if you trusted the source where it came from and the other issues like PM now that would suck. Weak plants like weak people i really got no time for them. Its like core values…if we have core values we wont get into situations where we get fkd over. Like the story i heard here the other day the guy had PM and told the other guy “its not that bad” WHAT its bad period! Right but to that guy because of his core values it really wasn’t that bad. So it comes down to our values. Some guys would have no problem dumping a load on top of some other guy in his GF…me i would have a problem with that. You see how our values play into all of our decisions…do i want weak plants in my garden? NO Its like women i don’t care how hot she thinks she is if she is a pain in the ass she’s got to go! I completely understand what your saying and i see your core values…Im not risking my health for a clone of some “killer” stuff.

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There may be off target overlap, but BTK is better at killing Lepidoptera and BTI for Diptera due to differences in the toxins they produce.

actually bandit does bring up a point about cloning. In the cloning test over a couple decades I generated a 100th generation clone from a clone they were all good. I really wonder IF they would lose vigor, but the thing I’ve noticed is grape vines can be cloned over hundreds of years span and still be ok. Oh and they are doing that kind of thing with strawberries. Just make sure all clones are clean :slight_smile:

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In my opinion, genetic drift is really just old, sick plants. If you keep your mums happy and healthy, the clone will be identical to it. If your mom is sick, has a virus, etc, the clone will also be sick.

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I got rid of mites using a similar technique. I chopped all my moms down to one tiny branch with a leaf and ran them under the shower every day for about 2 weeks. Much easier to keep them clean when they’re small!

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My “Protocol 0” is a 30 day quarantine. The quarantine area is 16"x16" tent that is setup in my bathroom.

  • Assume the cutting is contaminated.
  • Maintain a weekly IPM schedule including inspection with a microscope.
  • Never take in media, recut clones if already rooted.
  • Always interact with the quarantine tent last.
  • No shoes, hats, or glasses when working with the quarantine tent.
  • Always shower and change clothes immediately after working with the quarantine tent.
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@ReikoX ooof! yah you are describing the lengths I had to go to, in order to study the protocol. Bringing in infectious disease then playing with it, to see how it dies, is always risky. :stop_sign: DANGER! I put up bio-hazard signs and containment protocols. Then you are like that guy on the bone collector, someone’s knocking at the door to disrupt your experiment. Ooooooooo, now that gets me steamed. Call me names, kick over my buckets…but hell…never disrupt my experiments!!!

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I made some photos to go with this write up that shows the entire process. You can do it with plants with roots as well. Bigger plants… they are hard to fit in a sink :wink: You might be able to pull it off, but no guarantees. I’ll also explain how to construct a basic clean room. I made my own, but also got tips from Clair Cameron Patterson’s initial attempts to keep lead contamination out of his lab. If you want to watch the cosmos episode it’s pretty sweet.
Step one: get contaminated plant/cutting, with or without roots.


Step two: Strip the plant/clone down so there are no leaves. I also wash off the medium if it has some, but you can cut the clone right out of the medium if you really want. Roots optional.

Step three: submerge in water for 30min. Them bugs aren’t exactly…aquatic?

Step four: rinse off your clone/plant. I use a vegetable sprayer and rough handling :wink: I used the sink tap in the photo.

Now you just complete the cloning process. The plants themselves can take…lots of rough handling. As long as the stem isn’t destroyed and a meristem exists, the plant will live. The meristem is the tiny growth shoot sort of right on the side of a plant growth node. Yo, someone needs to upgrade the dictionary here to include technical terms.

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now, here’s the thing about the clean room. It doesn’t have to be super special high tech. It’s a system that anyone can enjoy! Exhausts and intakes have to be screened to keep out bugs! Tape the windows closed! Make an envelope in the growing area out of a membrane like polymer sheets taped together so there are no seams. You know how someone tapes vapor barrier and staples it to the walls etc. Just do that. OK! So the final tip once you have everything half-ass sealed up - remember we’re not grinding up pathogenic human remains - just keeping out bugs, now make 2 doors on your room. Put one door inside the other like you are making an air lock. One door seals against space, the other seals against the inside - if I need to be more specific about that just ask! The final phase is the human being. So you can be a vector, not just a plant you bring in. Bugs can’t open doors…as far as I can tell. Step inside the first door, so now there is a closed door separating you from the grow. Vacuum or brush yourself off. My friend, who is doing it indoors is obsessive about taking a shower and changing his clothes before going in - can’t blame a man for being paranoid!
If you need any more details about this process - just ask me. LOL

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crap I forgot the most important part! Print a large biohazard sign and put “PLANT BIOHAZARD” on the door to remind everyone not to go in and not to bring infected material in.

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I got some out of focus images of what happens to the aphids when they are sprayed with BTK. Also I have an image of a hoverfly larvae looking for a snack. The larvae are unharmed by the BTK - you can see them living and eating, even while the aphids are dying.

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You can see, those aphids don’t look so good. If you touch them, they fall off the leaf because they are dried out carcasses.

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forgot to mention, these images are from a pepper plant in the mega greenhouse. I built a hydro system out there the size of a soccer pitch.

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Spinosad worked for me

I had thrips and spider mites

2 applications of spinosad and poof there are no more bugs.

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meh, yah but protocol 0 means you don’t buy anything. I am using the BTK for it’s non-toxicity to beneficial insects. lol not in my grow. In my grow I will spray absolutely nothing on my plants except water. But water has no residue so I don’t really count it as “something”.

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I just love hoverflies and remember the day I discovered that something that looks so gross was eating all the aphids from the leaves of my carrots. The adult females go sniffing around your garden looking for the most aphids and that’s where they lay their eggs. Such amazing creatures :muscle:

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I was looking for a way to get rid of thrips on my blooming plants, but this peaks my interest… Have any pictures of your mega greenhouse? Would love to see it.

hell, yah! Got some ripe tomatoes and peppers going on there.


hah hah you can tell it’s something I’d do, all the plants are suspended from the roof by twine in that large drip system. Organic culture in the rubbermaids using alfalfa meal, blood meal, bone meal, earwig shit. All the good stuff.

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I suggest bare ass naked get your partner involved and have have fun in the flower tent! Lol :joy::wink::wink::wink::wink::flushed:

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Looks good man! Wish I had the space for such a garden.

So you rigged up a drip system? It’s not the ropes you see right? Don’t see no water tank or something hooked up.
Would take a lot of time to hand water all of that…