Hi Folks, SCJedi here to offer up a space where we can chat about the ins and outs of cannabis tissue culture. This is a safe space and there are no dumb questions.
I teach tissue culture classes in the Sacramento, CA area but would like to provide some basic information here on the primary types, components, and techniques that are required for success as well as a platform to ask questions for those heading into the tissue culture rabbit hole.
This will be BASIC and INTRODUCTORY. If you have desires about advanced techniques I will point you in a direction that you can research on your own. Somewhere between Intro and Advanced, I expect that you can download, read, and comprehend scientific papers so make sure to add that tool to your toolbox in between the two. I can also cover it as a topic here.
First things first, your success is determined by your personal tolerance for contamination. If you can get 1 out of 10 tries correct and that is fine, then you have succeeded. If you want 99 out of 100 to be uncontaminated and you hit that mark, then you have succeeded. Only you know what success looks like. With that said, consumables cost money, and your time costs money, so do your own cost-benefit analysis, please.
Tissue culture comes in two primary flavors; nodal and meristem. While there are some similarities these two techniques have advantages and disadvantages
Nodal: (Primarily used to store and rotate clean mother stock)
Advantages:
- Fast and efficient multiplication at scale
- Sterile and disease-free (if mother stock is proven pathogen-free)
- Genetic and phenotypic uniformity
- Reduced space requirements vs. traditional mother stock
- Reduced risk of maintenance of high-value genotypes
Disadvantages:
- Time (Growth condition optimization process R&D)
- Costs (Specialized Lab Equipment like a hood, scope, and autoclave)
- Skilled Labor Learning Curve (Learning/practicing aseptic technique)
- Losses (Contamination)
Meristem: (Primarily used to clean mother stock)
Advantages:
- The same benefits of nodal
- Have an isolated vascular system so lower risk of pathogens
Disadvantages:
- Much longer to culture ~10-12+ months start to finish
- Can still be susceptible to pathogenic infections - Mitovirus
- Requires greater technical & precision skills
- Mutation (somaclonal variation, i.e. not true to type)
There are multiple âstagesâ to plant tissue culture (PTC) that are in a Roman numerical order but DO NOT need to occur in this exact order, or need to occur at all.
Stage 0: Cultivation, Selection & Preparation of Explants
Stage I: Establishment of Explants
Stage II: Shoot Multiplication
Stage III: Rooting
Stage IV: Acclimatization
With that said, letâs dive into some of the basics.