Understanding breeding, how to achieve the best an strongest high, false beliefs an inbreeding depresion

Alright!

This is a good convo fellas but obviously I’m not going to address everything…

First I want to say that I have no problem with the common man experimenting with whatever line, using whatever resources he may or may not have. It does hurt a little seeing some commercially inclined experimenters starting to make good money and still cut corners.

breeding schemes

Lots of talk about numbers. The way I see running numbers is,1) if you are preserving a line and being any kind of serious about it, running 7 plants won’t cut it. Numbers baby, they don’t have to be big plants - nah nah- 100 plants, yeah good, but the more the merrier. Statistically, couple or three hundreds is cool. 99.6% genes preserved is good enough for me. (I did not do the maths, shits not that deep)
Or 2) you are looking for the extremes to move a line forward. (Transgressive segregation)

But to move a line forward? Albeit, slower - 30 per mating is a good start. It’s just progeny testing.

a graphic to control them all :joy:
42ko7ikr4lm51

gene frequency
Each method has its ups and downs

** anyway I’m out of time, this only the beginning :grin:
Note to self: sort your fking dl/screenshots dang it

Ttyl

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Is that you on ICMag? I got banned by Donald within one post…yeah great site ICMag :confused:

Naw, ghost protocol is banned Tom Hill, which is also banned :thinking: lol

I’m a nobody, hi!, I usually post my most recent work/rooms and change name every 2-300 posts.

Figured I could share a ghetto 2x4 and stick around :wink:

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How many have actually breed a plant until I breed depression has actually occurred?
How did you tell the difference in inbreed depression versus a slower pheno?
How many generations of inbreeding did it take to see any signs of inbreeding depression?
Once I slowed my plants down because I was under the impression that slower plants made bigger yield. I slowed the plant down by selecting and breeding slower phenos. In the end I got plants that took 3 wks longer and had no yield advantage. So of course I returned the plant to the faster producing version.
I learned this by doing and not just by listen to what others say. So it matters if you select slow phenos from an early cross. What really matters is that you are introducing slow producing genetics into your line. Inbreed depression? Or breeder unawareness?

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Few, I think people make a conscious effort not to go there, or lines are too multi poly hyb…

The first observable thing to give is vigor… but the difference between a slower line and a depressed one is mainly in its health. Depressed lines can be deformed, susceptible to pests, all the way to infertile…

As few as 3-4, and as many as 30…
The more inbred the starting stock, the fewer gens it would take.

Well, inbreeding depression is a real thing…
Does breeders and plants lovers alike spend too little time on their line, and are often too scared to trash a line and start anew from an earlier gen, maybe :thinking::sweat_smile:

Over (or close to) are the days when families stuck with a single or very few lines and worked it/them for decades…

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I appreciate the response brother.
I’m here to learn.

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Happy to help good sir.

And hey, I’m a forever student too, always learning :wink:

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Open Pollination only lets me NOT know who the father is.

That’s what I’m trying to tell you. You don’t NEED to know who the father is. Who cares? Forget about the males. Select the females in each generation that most closely approach the ideal, and reject the rest. Let the males open pollinate the females, sprout the seeds from the best females, and begin again.

So I have no way of knowing how to reproduce anything that may come from that.

The “reproduction” you’re looking for, i.e. uniformity and locking in positive traits, comes from line breeding over multiple generations. If you select the best and reject the rest, then over time your line will resemble the best, and not the rejects.

Preserving != breeding

Anytime you sprout a seed, you are breeding. Natural selection is always occurring at all times. The very soil mixture you use could allow some plants to grow and thrive and others to fail, for no other reason than the conditions are better for one or the other.

Breeding should have a purpose, a goal. If you just wanna preserve a line than sure, OP everything and call it a day.

Open pollination does NOT mean “no selection.” It just means quit trying to cherry pick individual plants to use, because you can’t do so effectively in the vast majority of cases, without causing problems in your line over time.

You’re not improving the line by open pollinating,

Say what? If you select the females that most closely approach the ideal and reject the rest, you most assuredly are breeding, and you are indeed improving the line over generations. And you are doing so without all of the waste of time and futility of trying to cherry pick males.

Even the ugliest male may have postive traits you NEED, but can’t easily test for. It might be ten generations down the road before that trait is truly needed (such as resistance to a certain pest or pathogen), and if you were happily doing heavy plant culling early on, or cherry picking males to breed with cherry picked females, now that trait is totally bred out and gone. If you had instead kept more plants, and just worked the line over more generations, you might find that later down the road when a disease strikes, only half the plants die instead of all of them, for example.

Every time you sprout a new generation, it’s like shuffling a deck of cards. The various traits (positive or negative) which the lines carries are shuffled around from place to place. The smart breeder does gentle selection over time to gradually lock in the positive traits, while gradually eliminating the negative ones he is able to test for. He damn sure doesn’t try to cherry pick individual plants and imagine he can guess, or figure out through testing, all of the traits that it carries.

If there’s a male causing deleterious issues in your OP line, now you have no idea which one caused it

You don’t need to know any such thing. You subject the population as a whole to various stresses, as does Mother Nature, and watch how they respond. The superior females are kept and the inferior ones are culled. If there is a trait that is clearly expressed in the male that is definitely undesirable (like weak stems for example), THEN you can cull that male. But don’t cull too many of them at one time, in a single generation. Don’t be rubbing the stems and all of this voodoo people do thinking they can use that to judge the male. That’s a fallacy for numerous reasons. Furthermore there are traits in both the males and females which you simply cannot judge just by looking at the plant, and would have to create elaborate tests in order to even perceive. You can’t just go culling plants based on the illusions in your mind about what is good or not, as every plant that is culled removes genetic diversity from the overall population.

Heavy selection does very much have the possibility to ruin the line completely from choosing that wrong male from the start

Even LIGHT selection is potentially harmful; see above.

the more plants you can look at, then the better chance you have of finding what you want sooner rather than later.

It’s about a lot more than “what you are looking for.” It’s also about what you NEED: like horizontal and vertical resistance to various pathogens for instance, which in some cases may be controlled by dozens or hundreds of genes. You simply CANNOT conduct enough tests to account for such things. Even if you could, it’d be a waste of time. You’re much better off in every possible way just by open pollinating and doing a gradual selection over time, than trying to cherry pick individual plants, especially male plants.

More plants are indeed better, which is why you shouldn’t be in such a hurry to cull plants, especially males, unless it truly has negative traits you can clearly observe, and only as long as you don’t cull too many in one generation.

All I can find and have ever read has said that Cannabis, like most flowers, are obligate outcrossers. They need to be outcrossed in order to improve the line.

Corn is an obligate outcrosser. Try growing only 40-50 corn plants per generation, and you will find your variety is quickly bottlenecked. The same does not hold for cannabis. You can start a new cannabis line with only one unrelated male and female. In the first few generations, maybe only 10-20 plants in the generation is enough to preserve enough genetic diversity for most purposes. 40-50 cannabis plants per generation is enough to maintain the population for quite a long time.

All those guys talk about heavy selection to pull out those original top shelf lines.

Of course. And they usually end up with a strain that has serious faults, like how Sweet Tooth #3 is extremely vulnerable to spider mites. That’s the price you pay for following the “conventional wisdom” of cannabis breeding.

Open Pollination only lets me NOT know who the father is.

That’s what I’m trying to tell you. You don’t NEED to know who the father is. Who cares? Forget about the males. Select the females in each generation that most closely approach the ideal, and reject the rest. Let the males open pollinate the females, sprout the seeds, and begin again.

So I have no way of knowing how to reproduce anything that may come from that.

The “reproduction” you’re looking from comes from line breeding over multiple generations. If you select the best and reject the rest, then over time your line will resemble the best, and not the rejects.

Preserving != breeding

Anytime you sprout a seed, you are breeding. Natural selection is always occurring at all times. The very soil mixture you use could allow some plants to grow and thrive and others to fail, for no other reason than the conditions are better for one or the other.

Breeding should have a purpose, a goal. If you just wanna preserve a line than sure, OP everything and call it a day.

Open pollination does NOT mean “no selection.” It just means quit trying to cherry pick individual males to use, because you can’t do so effectively in the vast majority of cases, without causing problems in your line over time.

You’re not improving the line by open pollinating,

Say what? If you select the females that most closely approach the ideal and reject the rest, you most assuredly are breeding, and you are indeed improving the line over generations. And you are doing so without all of the waste and time and futility of trying to cherry pick males.

If there’s a male causing deleterious issues in your OP line, now you have no idea which one caused it

You don’t need to know any such thing. You subject the population as a whole to various stresses, as does Mother Nature, and watch how they respond. The superior females are kept and the inferior ones are culled. If there is a trait that is actually expressed in the male that is undesirable (like weak stems for example), THEN you can cull that male. Don’t be rubbing the stems and all of this voodoo people do thinking they can use that to judge the male. That’s a fallacy for numerous reasons. Furthermore there are traits in both the males and females which you simply cannot judge just by looking at the plant, and would have to create elaborate tests in order to even perceive. You can’t just go culling plants based on the illusions in your mind about what is good or not, as every plant that is culled removes genetic diversity from the overall population.

Heavy selection does very much have the possibility to ruin the line completely from choosing that wrong male from the start

Even LIGHT selection is potentially harmful; see above.

the more plants you can look at, then the better chance you have of finding what you want sooner rather than later.

It’s about a lot more than “what you are looking for.” It’s also about what you NEED: like horizontal and vertical resistance to various pathogens for instance, which in some cases may be controlled by dozens or hundreds of genes. You simply CANNOT conduct enough tests to account for such things. Even if you could, it’d be a waste of time. You’re much better off in every possible way just by open pollinating and doing a gradual selection over time, than trying to cherry pick individual plants, especially male plants.

More plants are indeed better, which is why you shouldn’t be in such a hurry to cull plants, especially males, unless it truly has negative traits you can clearly observe, and only as long as you don’t cull too many in one generation.

All I can find and have ever read has said that Cannabis, like most flowers, are obligate outcrossers. They need to be outcrossed in order to improve the line.

Corn is an obligate outcrosser. Try growing only 40-50 corn plants per generation, and you will find your variety is quickly bottlenecked. The same does not hold for cannabis. You can start a new cannabis line with only one unrelated male and female. In the first few generations, maybe only 10-20 plants in the generation is enough to preserve enough genetic diversity for most purposes. 40-50 cannabis plants per generation is enough to maintain the population for quite a long time.

All those guys talk about heavy selection to pull out those original top shelf lines.

Of course. And they usually end up with a strain that has serious faults. All of this voodoo about “picking the best male” and such leads directly to that result. You might end up with fire, but you’ll just as likely to end up with a result like Sweet Tooth #3. That’s the end result of thinking “oh I’ll just select the males I like for arbitrary reasons, do a little testing of offspring from individual males, and pick the one line that looks the best to continue with.” There is far more traits that are necessary to keep in the line than just the ones you can easily see and select for, or in the case of males, IMAGINE you can select for.

It’s important to under that the vast majority of cannabis breeders–just like the vast majority of general plant breeders–just like most humans in general–HAVE NO IDEA WHAT THEY ARE DOING. That’s exactly how we got into the situation we are in today, where the market is flooded with shitty seeds with various deleterious traits locked in. That’s exactly what I’m trying to avoid in my own projects.

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You bring up some valuable points, (sometimes twice lol) but I get it. In retrospect we should pic the best females and use almost all the males. If we were to continue on with this reasoning, maybe save original seeds for B xing ?

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Ive always been conflicted with population numbers…from what i get out of a Punnet square is you only need about a hundred plants to start with, Mithridate likes 300, idk his name but ill call him faceguy…faceguy agrees with low starting numbers. I just don’t like seeing people saying
“you need 1000’s”
“ummm no? You look into any of this stuff or not? You dont need that many”

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You bring up some valuable points, (sometimes twice lol)

Yeah, that’s the forum software for you. Discourse is the worst fucking abomination. Everything about this forum software sucks, especially the tiny ass little window at the bottom of the screen they give you for editing posts. It’s obviously not designed for long, meaningful conversations between intelligent people, but for one-off throwaway oneliners from the typical hipster in the intended target audience.

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from what i get out of a Punnet square is you only need about a hundred plants to start with

Personally, I haven’t found the Punnet square bullshit to actually be of any practical use in breeding anything. It’s interesting as a 101-level introduction to the theory of how this stuff works, but I don’t find it to be of any help in day to day breeding usage.

It’s all about the genetic diversity in your population. Like I said, you can start a new strain (one that might stick around for a hundred years) just by starting with one good male and one good female of two diverse, well kept cannabis strains. If however you start with say CookieBoi and Corey Stardawg as your parents, your population is already bottlenecked to begin with, because the parents were. That’s why serious breeders working for the long term need to be crossing with landrace strains where the population has been well maintained in reasonable numbers over time, with a large percentage of variable, healthy genetics in the population, and then improve and maintain their own line in the same way.

Think about it like this…which would you rather have?

a) A population of cannabis plants that is uniform for obviously visible, desired traits (appearance, smell, high, etc) and highly variable for other important traits like resistance to various diseases and maladies, where each individual plant might have its own unique weaknesses and strengths in that regards, or

b) A population of cannabis plants that is uniform for obviously visible, desired traits, and also highly uniform in its disease resistance traits, meaning every plant in the population is either immune or vulnerable to the exact same conditions.

In the former case, an unknown or new disease might come along with half the plants are able to fight off and survive. In the latter case, may the same disease completely wipes you out.

The former case comes from slow, gentle selections over multiple generations, to gradually lock in visible traits. The latter case comes from conventional cannabis breeding methods, of cherry picking individual plants and using them exclusively for breeding.

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It’s all about expressing what we know, to people who do not. There’s preservation…then there’s breeding and i don’t like the two being intermingled cuz it’s gonna lose some people following you/us.
No you do not use the punnet sq. in breeding, you use it to bring the pupil into your mindspace and show them…see these are about the only outcomes you can expect from this pairing. If the parents are polyhybrids yeah ur fkt, good luck have fun but if they are f6’s the story changes as a result in a punnet sq.
For breeding an entirely diff set of criteria are used when held to a self sustainable high population.
Need to delineate which avenue applies to which pursuit.

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How to start such a process? What you’re saying is already further down the road

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There’s preservation…then there’s breeding

I don’t do any “preservation” at all, in the way you’re thinking. No one really does. As I’ve said elsewhere (in the thread which was locked for no reason), preservation is a myth. You can’t preserve a strain exactly how it is over any kind of long term, forever and ever. IF you arrive at the desired traits by gradual selection over time, and do open pollination of sufficient numbers with each generation, you can keep something going for a while, but cannabis is constantly changing with every generation, as it must in order to survive. The fallacy comes in thinking one can just pick out a certain set of visible traits one likes, then force the plant to fit that mold by cherry picking and breeding with individual plants, and “preserve” it. This causes irreparable harm to that line over the long term, and eventually it will lose vigor and become extinct.

Sure, I can do heavy inbreeding and wind up with a cup winning female or a bottlenecked IBL in a short time frame. But will I be able to keep that same strain exactly as it is over the long term? No, as any of the breeders who have come and gone over the years can tell you. DJ Short is trying his best to bring back Blueberry and such exactly as it was, but that’s never going to happen. That line is played out and gone. At best you can take a good survivor clone and outcross it to a landrace, inbreed it, and “stabilize” a new population with the same trait. But then you’re probably right back in the same boat of having a heavily inbred line that won’t last over the long term.

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…i like this guy

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You can’t preserve a strain exactly how it is over any kind of long term, forever and ever.
I thought that’s what we were on? That’s what we think you can do if you start at 300.

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How to start such a process? What you’re saying is already further down the road

The best general approach to building your own strain over the long term is to take a really good elite cut that you like, then take some males from a good landrace strain–meaning one that has been traditionally kept over a long period of time by gentle selection in a reasonably large population over many generations (say at least 50 plants per generation, male and female, with more being ideal)–and cross the two. Grow as many plants as you can per generation, aiming for at least 40-50 plants if possible (both male and female), although you can get away with fewer in the first few generations. Do little to no selection on the males, and try to keep as many females as you can each generation.

Instead of looking for that “one special female” or “one special male”, just let the males be for the most part, and try to pick out say the top 30% of females, the ones that overall do best in your environment and come closest to your ideal, and/or seem to have some desirable traits that you would like to preserve in the line, and use those seeds to begin the next generation. Repeat this strategy over several generations and you will be well on your way to creating your own interesting line that is not only good smoke, but which remains genetically diverse, healthy, and vigorous.

The main thing to keep in mind is to take the SLOW road, doing as LITTLE selection as possible. I always collect all seeds from all surviving females, and label and document them in my grow log, even if I’m pretty sure I won’t be using that particular female to breed anything. It could very well be that your thinking changes later down the road and you end up having a use for those seeds after all. If nothing else, you can share them with friends, and they might end up getting something really nice out of them, or use them as a start to their own line breeding project.

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Definitely wasn’t trying to bring it there. I actually am thoroughly enjoying the conversation. So let’s bring it back. My question was should all of our selections be from the female representations and use any male with disregard to almost anything ? And if that’s the case, should we keep original seed stock for backcrossing.?

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The rant about the forum software wasn’t aimed at you; it’s just part of my general feeling of absolute hatred for the garbage that is the “modern” internet. Even VBulletin, which is itself was shitty forum software, is light years better than this Discourse crap. Never, ever thought I’d be wishing for VBulletin instead of something else, but here we are. Never understimate the ability of humans to fuck up and destroy everything they touch.

Now about males, there are certain important principles to keep in mind. People talk about growing a million plants or whatever because they understand on some level that numbers are important, but it’s not because you can select the exact phenos you want out of a large population. My point is you really CAN’T do that, because you never can see exactly what the plant has to offer just by studying it, or even growing out its offspring and “testing.” There are way too many traits that remain hidden beneath the surface which are equally important, but which are difficult or impossible to test for. So the #1 goal should be to do as little selection as possible, just the bare minimum to slowly improve the strain over generations, while counting on the “card deck shuffling effect” to reshuffle the genes with each generation, so the result is the overall population improves with each generation, in both ways that you can see, and ways that you can’t.

Since it is the female plant we smoke, and which actually display traits that we can select for such as bud appearance, potency, etc etc etc, that should be the prime focus of our selection efforts.

Now if you’ve got 20 males for example with varying characteristics, and then one of them turns out to have a rather weak stem and it blows over in the wind, there is no harm in culling that one plant. If you see something that you can select for, then select for it. But if you’ve got 20 males and 19 of them have weak stems, DO NOT cull 19 males and keep just the one with a strong stem. Maybe cull up to 50% of the worst ones, perhaps, or even just 25%, then “shuffle the deck” by growing out the next generation, which will have improved a bit. If you keep this up, working slowly over multiple generations, rather than trying to accomplish all your goal in one or two generations, that is the key to maintaining maximum genetic diversity for those unseen important traits, while gradually working the line towards the ideal.

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