Making some polyploids

Very interesting that some people these days did colchicine experiments as well :+1:

I inherited 2 Cannabis-Books of my father which were written in the 70s and in one of them the colchicine method was explained. As everybody with some DNA-Skills knows polyploidization (DNA-Multiplication) in already built up bidiploid cell-structures will cause space problems in the cells which triggers more or less ugly mutations. Furthermore colchicine does not copies just floral DNA it could copy human DNA as well which makes colchicined weed unsmokable.

But there is another way:
I spayed colchicine daily on pollinated buds. The goal was to multiply DNA-Structures while they were build together from the female and male plant. Some DNA-Structures would be copied more often than others, which would lead to more genetical variations in the offspring which could be selected by own desire in the upcoming generations.

Long story short, it worked. The Seeds had a pretty good germination-rate of 90%. About 60% died (life-important genes were not sufficient multiplied, compared to others) in the following days and weeks but the rest had pretty interesting variations.

Like: Smaller/Bigger buds, Less/More trichomes, Leaner/Wider leaves, More/Less bushy growth, Purple/Black/ordinary Green buds while flowering, some of the plants were weak to mildew, some were resistant to mildew etc.

The colchicine treatments brought up almost every genetical phenotype of a cannabis plant we know. Certainly not everything, as a Thai Sativa has a number of unique genes that a Skunk simply does not have.

So, colchicine is a very good way to extract desired genetical attributes of a Strain* and could be breeded/stabilized in further generations. Sure I cannot prove it, but maybe Big Bud was invented this way, as colchicine was quite popular in the 70s and this strain is for 50 years of breeding sadly unstable (10 seeds - 8 phenotypes)… maybe that’s the reason.

  • I used Skunk #1 from NL - Sativa dominant
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Hi @engine you might enjoy this brand new journal article from this year about ploidy and colchicine treatment, it’s very good! Neat to meet someone who’s tried it:

This is really interesting. I have tried different methods for years. The colchicine dip is not the best and I have found rather than trying to make polyploid seeds that it seems easier to mutate seedlings that have already sprouted.

The thing is that quite a lot of them die but the ones that make it can defintely have some good yields. Some people say its no good to smoke it once treated but the times ive done it it was no problem like I didn’t grow and extra ear or anything like that.

I did a fair bit of reading before I started here’s some of the better info

Floraflex Polyploidy

Making Polyploid Seeds

Understanding Triplod & Tertraploid

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I’ve been interested in the triploids, but as a real new grower, I have lots to learn but in my reading another way is doing anther culture and doubling from there to achieve faster homozygosity while creating the tetraploid as well.

also I have been looking to get some Gloriosa superba but if anyone knows an easier/cheaper path to colchicine dm me

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