Very interesting that some people these days did colchicine experiments as well
I inherited 2 Cannabis-Books of my father which were written in the 70s and in one of them the colchicine method was explained. As everybody with some DNA-Skills knows polyploidization (DNA-Multiplication) in already built up bidiploid cell-structures will cause space problems in the cells which triggers more or less ugly mutations. Furthermore colchicine does not copies just floral DNA it could copy human DNA as well which makes colchicined weed unsmokable.
But there is another way:
I spayed colchicine daily on pollinated buds. The goal was to multiply DNA-Structures while they were build together from the female and male plant. Some DNA-Structures would be copied more often than others, which would lead to more genetical variations in the offspring which could be selected by own desire in the upcoming generations.
Long story short, it worked. The Seeds had a pretty good germination-rate of 90%. About 60% died (life-important genes were not sufficient multiplied, compared to others) in the following days and weeks but the rest had pretty interesting variations.
Like: Smaller/Bigger buds, Less/More trichomes, Leaner/Wider leaves, More/Less bushy growth, Purple/Black/ordinary Green buds while flowering, some of the plants were weak to mildew, some were resistant to mildew etc.
The colchicine treatments brought up almost every genetical phenotype of a cannabis plant we know. Certainly not everything, as a Thai Sativa has a number of unique genes that a Skunk simply does not have.
So, colchicine is a very good way to extract desired genetical attributes of a Strain* and could be breeded/stabilized in further generations. Sure I cannot prove it, but maybe Big Bud was invented this way, as colchicine was quite popular in the 70s and this strain is for 50 years of breeding sadly unstable (10 seeds - 8 phenotypes)… maybe that’s the reason.
Hi @engine you might enjoy this brand new journal article from this year about ploidy and colchicine treatment, it’s very good! Neat to meet someone who’s tried it:
This is really interesting. I have tried different methods for years. The colchicine dip is not the best and I have found rather than trying to make polyploid seeds that it seems easier to mutate seedlings that have already sprouted.
The thing is that quite a lot of them die but the ones that make it can defintely have some good yields. Some people say its no good to smoke it once treated but the times ive done it it was no problem like I didn’t grow and extra ear or anything like that.
I did a fair bit of reading before I started here’s some of the better info
I’ve been interested in the triploids, but as a real new grower, I have lots to learn but in my reading another way is doing anther culture and doubling from there to achieve faster homozygosity while creating the tetraploid as well.
also I have been looking to get some Gloriosa superba but if anyone knows an easier/cheaper path to colchicine dm me
Thank y’all for sharing that information! Very interesting things you made there. I wonder if you managed to keep that genetic alive wether from cloning or reverse sex pollinating.
Also I’ve read doing that outdoor would increase the chance of the polyploid cells to actually be alive by a ton. Due to more complex lighting(sun) and the electrical grounding & microbiomes. Living Soil would be best since it can buffer the stress caused by the unsual genetics. They have increased metabolic demands aswell as are more sensitive to stress due to genetic complexity
I’d appreciate any thoughts about that, wether you think its a good point or not.
Edit: I found out that 400hz for 30-60 minutes a day increases the amount of cellsplitting (resulting in bigger growth overall), which means in combination with oryzalin/cholcicin would increase the amount of cells getting affected by their effects, maybe one can try that, even tho it should at least be 6h-24h a day so it has actual effects on oryzalin injection.
I’ve never made my own but very intrested in this stuff. I’ve just finished my second harvest and ran a couple triploid strains in my first one. Working on making my own soon. Just reversed my first auto plant and waiting on pollination to start now.
That’s funny I was looking for potassium malate and only one Amazon supplement came woth it but with a ton of taurine which i think is an amino.
I had grok calculate the caffeine in mg/g average for instant coffee powder i need to find it again.
Thanks for the guidance! Great find!
