Paper on artificial seeds and their viability of between 24 hours and 60 days for various crop. Cannabis is listed but does not note viability: agronomy-07-00071.pdf (1.5 MB)
This master thesis discusses a variety of topics including tissue culturing, encapsulating a tissue culture (synthetic seed), and encapsulation-dehydration. Their study seems to indicate potential viability at 180 days but until I pick through the paper itās not entirely clear. They also discuss quite a few other interesting topics including trichome induction: Cannabinoids production in Cannabis sativa L.: An in vitro approach
Have you any experience/success with tissue culturing Cannabis? And if so do you have a regime that works? Itās on my todo list but working out a successful regime is likely to be a reasonably long winded processes.
I have some experience tissue culturing of orchids, but to do it properly requires a clean room and/or a fume hood and well I already take up enough space in this house with crazy experiments lol
The process itself is straight forward enough, itās the regime of hormones that takes some working out!
Polyethylene Glycol (PEG) is used to encapsulate. As you say the there seems sufficient info to have a crack at it. PEG is also used for mediated cell fusion when creating somatic cell hybrids. This is something else that woudl be worth looking at if there is a lab handy
Iāve acquired (most) of the equipment and the supplies including hepa filters for a laminar flow box, hormones, sterilization equipment, etc. Just been real busy with others things and havenāt gotten around to running through the paces.
The agronomy paper notes (for the callus induction):
MS medium +
3% (w/v) sucrose +
0.8% agar +
0.5 mM (TDZ)
Here is another procedure document (more specifically tuned micro-propagation but the multiplication stage should be the same): c1850-info_1.pdf (524.7 KB)
For the multiplication medium, in their example, they note something similar along with the addition of NAA (1-Naphthaleneacetic acid).
Reports are that a small amount of plant preservative mixture (PPM) added to the media has been successful for the less than perfectly sterile environments, as well.
Itās more vicious than that, but the base of the thinking is good.
A breeding program is nothing more than a game of leverages. Understand than by āleveragesā the drastic increase of āforcesā moving the given genpoolās averages in a way or another : letās say blue buds.
Warning : i expose very partially a method, but itās more a simplified example to make this concept more clear.
Example : You start with unlimited F1s (because you produce them with P1) and you directly kill everything than is not producing blue buds in a large scale (with donāt necessary mean a large space), no matter what. After a year, you will finish with a ācompleteā set of āblue budsā specimens of the genpool. You have to understand first than it is true for a farmer, but not true for a genetician. Iām considering myself as more close to a farmer than anything else.
What ācomplete setā mean ? In the spectrum of the āblue budsā expression, you will have a panel of different specimens than carry this specific expression. Letās say than somes will be strong and blue, tasteless and blue, vigorous and blue etc ā¦ this new genpool in only one generation can take naturally hundreds of years with chances to āpopā out from the given genpool which are ridiculous.
Thatās the big difference between an open pollination and a breeding program : leverage, then time spended.
Itās useless i think with the level of this post to explain the major priority than is playing resistances, vigor, early effisciency in term of photosynthesis and root development etc ā¦ in an open pollination.
Seeds always sowed in February for my birthday lol, old stupid tradition ^^ but not entirely ādecidedā for the fun of the act.
To donāt expose an absolute equation which lead to think than open pollination is a bad tool and is to avoid, i was (unfortunately) āwasā using it a lot for my IBLs in the past. I thrown the seeds directly in the ground in a corner of the garden (no pre-germination, no nutrients), and let the most resistant specimens drive the dances. But only to āre-invigorateā the highly pressurized lines and eventually to acclimatize it in a given lattitude. The leverages of the breeding was allready working at full throttle in these conditions, so it was not too much destructive for the quality of the buds.
To conclude, i will repeat : Natural selection is not a magical spell than is never failing. Itās exactly the reverse, for one successfull-resistant line you will have a āgenocideā as the price. In term of time we are also speaking about multiple human lifespan.
Now you force me to extend my comment to the inherent sexual equilibrium of our ganja ^^ I will keep it short to donāt put your brain in a blender with tons of concepts to digest at a time.
Sexual prevalence with cannabis exist, but it change with the lines and the āleveragesā you are juggling with. A good farmer will always first consider the males like the ābrothers of their sistersā and nothing more, INSIDE the given line.
The āvalorā of a given sex for hybridism is another game, to donāt mix in mind, and mainly focused on the F1 production and concern mostly the quality of a given sex of a given line when itās facing the gens of a distant genpool.
I consider my Skywalker ibl like a āmaleāsā line. You cross any weak female with it, even dominant, you end with a narcotic smoke. But the males weight in the inbred selection is equally important than their sisters, if i start to only consider males in this line i will just fade the line in term of potency iām wanting to increase each generation and maintain like that.
In the same vein, it exist ārulesā with core lines in term of hybridism. Using a JH male always lead to extend the effect of a given weed, no matter if itās good or not. The use of a MKUltra male will lead to barely the same scenario of the Skywalker, the use of a pure/true Skunk male will lead to increase yield and vigor, the use of a pure/true WW will always lead to dominate the cross expressions ā¦ etc ā¦ etc ā¦
BUT donāt take care to donāt mix the education on core lines, and the sexual equilibrium of males against females in a given line. Thatās twos different concept and mechanisms.
Thanks buddy, but happy to be retired to be honest ^^
I share you point of view for these news techs, not because iām a āfarmerā in the core and than these kind of shortcuts donāt please me. With good intentions and balanced knowledges (equally āfarmerā and āgeneticianā), it can lead to a true resurrection of the weed iām loving the most. The one not damaged lol
But because iāve saw years after the years the high damage of the revolution than was the feminized seeds in the global genpool, and than i known a bit now this business in backstage ā¦ i have no hopes lol
The time to store seeds is over since now barely 20 years, itās more the time to maintain them fresh and alive imho. Itās why also i donāt reject this pollen bank idea in the absolute.
You can find an extensive and exhaustive documentation (more available) on it in the bamboo industry, if any. It look like futurist like that but itās old.
Not at all, at the origin. And in term of laws itās very interresting. But we will find a way to screw a bit more the genpool with it iām sure ^^
Take care to consider PEG with its grade only. A PEG400 will be totally useless, you need to go for the purest and higher grade PEG~4000-6000 you can find (mostly south Korean or Japanese).
KudzuOG
