If you’re interested in some general TC info this is an excellent resource. They start with mixing your own media from scratch, and take you right through to roots. It’s got a lot of lab activities as well. None of it’s canna-related, but it’s got some solid basic info.
Plant Tissue Culture, Techniques And Experiments.pdf (2.0 MB)
If you’re really into reading, here’s a link to a zipfile that has a lot more in it: CannaTCInfo
Very in-depth information that highlights the biology very well. Practically a college lab book. Thanks again, appreciate all the solid info!
I’m becoming a bit too obsessed with making these boxes. Guess all of my storage totes will have holes in them lol.
I use the isobutane / propane canisters (for camping) with a chinesium bunsen attachment (via ebay / amazon).
Someone watches AvE…
It’s really easy and cheap to make an alcohol burner stove. Being a home distiller I tend to have lots of undrinkable high proof liquor(heads and foreshot, stuff contaminated with methanol and other undesirables) which makes for great fuel. They’re fun to design too and you can adjust the flame by altering the amount/size of holes that you put in the top. Here’s a link to an REI article about making them https://www.rei.com/blog/camp/diy-how-to-make-an-alcohol-stove-from-soda-cans
@JustSumTomatoes can you do tissue culture with the small cotyledons from a germinating seed?
On old seeds they sometime don’t form leaves but do get a green cotyledon. It might be a way to save some of these old seeds in our collections…
I was looking at those and almost got one. This is what I ended up getting as it had pretty solid reviews. I like that the flame base is very wide…
I’ve never tried it, but that’s a good idea. I’m still learning the ropes to this so I’ll definitely have to give that a go.
That’s perfect! Thanks for posting. 
I’m in the same boat, lots of heads and foreshots. You running a column?
Cheers
G
It should be possible via callus IMO
I generally just run my pot still tbh. I have a VM/LM combo that I built but I prefer running the pot still for whiskey, rum and brandy which is generally what I make
Yes you can. 
Rapid Shoot Re-Generation From Cotyledons.pdf (1012.1 KB)
TDZ And BA In Vitro Cotyledon Explants.pdf (700.3 KB)
@Guitarzan I’ve never tried taking cannabis explants, is there a certain part of the plant that is better than other parts for this?
lol
better copyright that or I’m gonna steal it!
All yours, though I think AvE may have coined the term 
Shit man, I missed your post til just now. 
As far as explants go, you can use leaf, stem, or nodal explants. Which one you use depends on the media recipe. If you’re working with leaf or stem material, then this doc is the one you want to use: High Frequency Cannabis Plant Regeneration from Leaf Callus.pdf (171.7 KB)
If you have plants that you can take nodal explants from, then you’d want to use this document TDZ-Induced Direct Shoot Organogenesis of Cannabis Sativa.pdf (853.9 KB).
The difference is time. The Callus route takes 3 different stages to get to a rooted clone. Callus is stage 1, then shoots, and finally roots. Each of those stages requires a different media formula, and about a month at each stage.
The TDZ method removes the callus stage, and goes directly to shooting, hence the name, Direct Shoot Organogenesis. With this method, you cut out nodes that have a 5 mm or so shoot growing from them. After they’re sterilized they go into media, and in a month or so, you’ll see new growth. Then comes rooting, which is where I have failed every time I have tried it. I have yet to figure it out, and I’ve been on a TC hiatus for about a year.
But I cooked up some TDZ media, I have some clones going, and they’ve got some good nodes on them, so I can see firing it back up soon.
Great sharing
Are you aware of the site Shroomery? It’s devoted to mycology and has vast info on still air boxes, to hepa filter setups called laminar flow hoods. I feel like some of you might be trying to reinvent something already reinvented over and over by many in the past and well documented.
