So far these are what I’m considering
Setting up a Athena tissue culture kit. Plan is to backup favorite strains for rotation, preservation and cleaning for diseases using Meristems.
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This is an interesting product from Plant Cell Technologies:
Found an example of use. Her channel is excellent, as well:
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That BioCoupler looks like something that might be 3D printed…
Cheers
G
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You could probably if not using an autoclave. Maybe Starsan + PPM would be sufficient.
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The starsan that is used in brewing for disinfectation?
I think that it eliminates bacters and fungi but I’m not sure about yeasts.
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Very cool! I didn’t know u were doing this! Thanks for sharing @Northern_Loki im looking into getting into this as well. Awesome
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Me too 
I’ve tried a couple of procedures and have most of the equipment, ingredients, etc. Except for a flow hood, which I’ll just use a tote.
But my utter laziness has continued to get in the way.
What is it now … something like 3 4 years
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Thank you for this. Bookmarked
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Yes. Just noting that there may be a sanitization problem, idk. I’m not up-to-date as to what temperatures/pressure that 3d printing is capable of.
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That is interesting.
I would be interested in making some. I would worry about algae. The clear plastic. I get it in a few things is I don’t keep clean.
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They are ~$17 per coupler. Works with mason jars. Or they can be bought with the jars included.
I might pick up a couple (which will then be placed on the shelf to look at for awhile) 
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Yeah, we get algae often here. I think it would be ok though.
The set-up has to be extra sanitary. It’s most likely under low intensity lighting / relatively controlled temperatures. Everything gets autoclaved or somehow sanitized. Maybe it’s possible to pick-up algae off the plant matter? Though, that’s being sanitized to an extent too.
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DIY bio coupler. The black tube is the vent. Stuffed with Polly wool. Have to keep it dry. ECSD explant in there barely visible there is a stainless screen keeping the plant in the top.
No algae grows. You have to sterilize your explant or anything else takes over.
Here is a pre 98 bubba s1 premature seed. I should have cut out the embryo I’m guessing. This was just and experiment to see if it would germinate.
I’ve been playing with meristem a as well as beer yeast and funji. Fun fun.
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I just built one of these for my mycology hobby.
my two cents.
Very interesting thread.
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Mine cost a little more. I build a melamine box, and used a Hepa 14 600x300, and put also a UV sterilizer light.
Works pretty well… but I miss more space. Probably will make a bigger one, I have a 900x600 H14 waiting for time to construct another box…
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Nice. HEPA laminar sure makes things a bit easier.
I have an old 3x4 laminar hood.
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Pretty much had everything lying around the house. Except for silicone magnets and the plastic placemat.
Jar of shitaki liquid culture to the right.
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Where can I find the liquid nutrients? To do this?
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