Been reading alot on tissue culture lately. The idea of long term genetic storage is exciting how ever currently there are problems with genetic sporting (mutations in the dna) that are more likely then with traditional cloning. Seems to rather defeat the purpose. It is very interesting that a plant could be cloned from the smallest sample though. People can just tissue culture the wet stem in a nug and acquire coveted non released strains.interesting world of weed we live in now a days.
Do you have any information on TC Mutation. I’d love to hear more about this topic.
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Nice post. However a couple of things stick out. From what i understand, there is little genetic drift from tc. Also, as far as cloning a bud to a plant… is it POSSIBLE? Sure. However, the knowledge/ skill set involved seems to be way more advanced that the typical snip n stick cloner possesses. Also, getting ahold of the nutrients needed for this is not the easiest. I have taken an interest in TC lately and there is a lot to be learned. I’m having troubles even finding what nutrients/ media I need to keep a culture in a static or ‘preserved’ state
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Tc caught my eye too… the failure rate is high according to Kevin jodrey. For all the work involved, personally it’s just easier to go the clone route and Chuck any large plants, or give them to friends.
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One major reason for failure is lack of sterile method… personally I have been able to flask orchids in a repurposed fish tank, but yes, if not sterile you will also breed toxins successfully
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@GrapeApe mutations occur due to stress on the cells from the methods involved with long term storage(slowing growth to almost a standstill) and the hormones/chemicals used in tissue culture. Here is a link to a more in depth article that cover this
Genetic instability of plant tissue cultures: breakdown of normal controls.
https://www.ncbi.nlm.nih.gov › articles
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@Badfishy1 p-fluorophenylalanine is one thing that is used along with many others,
for extreme long term storage you are going to need to look into cryogenic freezing and the products used for each stage of tissue culture are different and the amounts of each are up to debate. If you google tissue culture for genetic banking in plants alot of good articles pop up, this technology was pioneered back in the 1970s and is still be studied extensively today. I see alot of promise for starting seeds that wont grow otherwise but I think freezing seeds cryogenicaly would be better then use them to tissue culture if unable to germinate. They grew a plant from 30,000 year old plant material that had been preserved in ice so I think growing from a relatively fresh nug is very possible.
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Absolutely no denial it is possible! Issue becomes amount of knowledge and access to the tools to do such a thing…
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Yes knowledge of proper lab practices and procedures is key and some decent kits are available in the 150 -$200 price range but that does not include many other things used , somebody could play around with it but to get into any large scale operation or to create a large preservation bank would be expensive and time consuming. There are companies though who offer genetic banking of germoplasm and there are small scale storage units available but again were talking big money
- Personally don’t think TC will become a norm in the foreseeable future. I am talking to a friend on another forum as he is trying to produce a self contained kit for TC and just received his first shipment of a ‘proprietary’ solution aimed specifically towards the canna community. To me, the value in genetic preservation is never having to give up a cut due to lack of space. Would be awesome having 1000 cuts in a fridge to go back to at anytime a person desires. Or even keeping a male around after he has been used for a certain project to bx to in the future
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And yes, I’m sure skunk man Sam would love to get the 50-100kg of original skunk that he is sitting on to be viable at some point in the future. Bc at the moment he is sitting on 1kg of viable seeds and 49kg of paperweights
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Couldn’t agree more about finding the nutrients needed. I’ve found this Plant Media but I’ve yet to order from them. Also a book that might interest some https://investigacionfitopatologiaumar.files.wordpress.com/2016/06/plant-propagation.pdf
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I’m pretty sure sam has the funds or support to access the type of labs that would be able to germ old seeds threw tc methods,the germination rate when using sterile agar and growth hormones is much higher and if not the cells contained within may be enough to tc from them alone. There is alot of info on the subject but it is contained in scientific documents that require at least a base knowledge of plant biology to read and understand fully. Having a sterile environment is key and the steps taken In a lab to insure this are not complicated but must be followed , over all tc has purpose but is much to cost and labor intensive to replace cloning. I would how ever say that the chances of getting old seeds to grow is getting better every day so hold on to those gems
Here is the link to the document that describes in detail how tissue culture was achieved with 30,000 year old plant cells . It contains alot of info on the different chemicals used and why. Hopefully one day we can have access to this type of science at a obtainable price
I’ve ordered from Phytotechlab. Professional company. A bit on the expensive side but you do know you’ll be getting exactly what you’ve ordered. They have nice culturing vessels and closures.
I’ve purchase plant hormones and such off Ebay at 1/10th or less the cost, as well. But, there is always that nagging feeling that you are never quite certain…
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Tissue Culture represents opportunities from a preservation perspective but is a double edge sword. It may also accelerate genetic streamlining and appropriation by biotech companies…
This article details the pros and cons
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