Thought i’d post my process here copied from a comment I made on reddit that might help someone
For my agar I use water I had used to simmer the rye grain I was prepping for my jars. I filtered that through a coffee filter and then took 900ml of it and 20g of agar agar powder and mixed together. Boiled for about 15 minutes whilst stirring which reduced it to about 800ml. I poured 200ml into 4 glass bottles and pressure cooked for an hour at 13psi.
So these were transfers from other plates I had growing. My process for making transfers is to set everything inside my SAB that i’ve wiped down with disinfectant. Wipe down my table, my scalpel handle, the dishes im transferring from with iso alcohol.
First i put a clean blade on my scalpel and flame sterilise until red hot, then open the receiving dish and press the blade into the edge to cool it, put the lid back on. Open the first plate and cut a small section. I find it easier to just press the blade in and then rotate rather than cut. Poke the wedge and lift out, put on the receiving dish and then set aside. Wipe down the scalpel with alc and then repeat.
Then I wrap in parafilm and store facing up for a day or so, then flip upside down.
For spore syringes its pretty much the same process except you squirt a few drops onto the agar. You sterilise the needle until red hot then squirt a few drops across the plate. Then isolate using transfer process.
You can also just scrape spores from a print directly onto the agar and it will grow out.
The pictures labelled S are originally from spore syringes where the ones labelled T are from a mushroom tissue sample.