Yes, root pruning does work…but I don’t keep marijuana bonsai I just make new mothers.
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No mothers for me either. Each new clone gives new clones the day before flower… for evah!
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It was more of an experiment. The plant wasn’t in good shape, and I wanted to keep the strain, so since I had 2, I up potted one, and practiced the other for making clones once it recovered. I couldn’t have gotten any clones, both were too root bound.
Getting them healthy again when root bound can be hard, but mine that’s been in a 4” container for the past 5 months still produces viable cuttings that root in a week. But key to what I’m doing is keeping it pruned to a certain size.
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You can clone tomatoes…@MysteryMoog
I know…THOSE are clones …lmao
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I know you can clone peppers. I have e nice pepper plant right now that was a clone from last year’s bell pepper plant. Have already taken a few peppers from her.
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If it’s the original ECSD you are a lucky human
I saved cuttings from some peppers last fall, but I didn’t have the space for them, gotta start new ones this year.
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And sheep!
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Cloning sheep…in case you are so inclined…
apnews.com › article
Sheriff Accused Of Stealing Sheep Goes On Trial - AP News
I got to tour one of our organic commercial grow ops in town. They do a whole giant room of clones off of one plant. Each new batch is rinse repeat. Like someone said above, it’s the only way they can get a consistent product. They do it over and over. Pretty cool to see in a commercial setting but it all looked much more like a boring job than an exciting adventure when done from your home. A whole giant room of the same exact plant. And, they only veg them for a very short minute. All budded plants were only about 14-16 inches high.
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You nute, correct? It’s a bit harder to do that in living soil. I’m learning some tricks to stretch it out though.
With the bonsais yes, I use bottled nutrients, dyna grow currently.
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_Growbynumber_sub_clone_of_a_clone (please remove this line if you quote this message)
Hi, Interesting topic … Heres my 2c on it.
While I do keep my eyes and ears open for such articles etc. I have not heard of any studies on this.
Short of a long story is that it is most likely ok AS LONG AS THOSE CLONES ARE NOT ALLOWED TO PRODUCE SEEDS. this would include also giving clones to your friends (as they might be used to produce seeds further down the line)
My educated guess on the subject:further more … Life cycle of plants is long so number of iterations of cloning will allways me linited.
While clones should be identical, there is all ways a small risk that something within dna changes. These changes are very very small and it would require dna sequencing to find out.
lets consider virus. virus copies itself and dna should in theory be identical. As we see with covid-19 … there is actually abundance of changes within dna. It is only with huge population (people infected with virus) and huge number of cloning iterations … that we now see changes that can be observed without sequencing (changes in spike protein in new more contagious variants)
is there changes in clone of a clon of a clone…
So NO - in theory there should be no changes.
but YES - there will likely be changes here and there.
but further NO - these chages are only observable with dna sequencing and not with naked eye.
but yet again NO - The minute changes will play a role IF these plants are used to produce seeds. In that case the minute changes will become part of the new strain.
This is more of an academic issue.
difference between original plant and 100th clone of a clone should be much much less than 1%.
So IF one dose not have the ability to keep mothers alive … clones of a clone is valid path to take - Just to be on safe side - keep that line contained and do not allow it to spread.
Its spread been brought up, but most of clone off clone degradation is due to plant health and viruses.
What hasn’t been brought up yet are sports. 
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I’ve been working from clones of clones of clones (etc etc, ad nauseum) the same mothers for 20 yrs now. If anything, due to better technology, it’s improved over the years. I would feel safe in saying that if someone had a problem with the quality or such, it would almost have to be from cloning a weak or sick plant. The best of the best I keep as mothers and some are 5,6,& 7 yrs old themselves. But they too are still just clones.