And finally, the long delayed germination test and results…
Germination Test, Cool Runnings seeds
The germination protocol is:
- 2 hour soak in 75ppm H2O2 solution
{NOTE: further research indicates that it should be a 1% solution of H2O2}
- 96 hour in fresh EWC.(earth worm castings)
I cleaned and prepped a standard Chinese takeout container, added 1/4” (6mm) of fresh EWC
as described by @JohnnyPotseed in his essay on the subject.
I’m testing 20 seeds each from the three females. These are organized in double columns of 10 in the container.
Each seed is pressed in slightly into the EWC (pressed about halfway in)
24 hours = no activity
36 hours = some indications of germination activity
72 hours:
17 A5 have popped
11 B5 have popped
15 C5 have popped
96 hours:
18 A5 = 90%
12 B5 = 60%
17 C5 = 85%
The results are frankly surprising, I was expecting much lower germination rates. Out of the three females I thought the second one, “B” would have been higher than “C” (based on plant phenotype characteristics)
An explanation of my nomenclature:
I had 3 females and 5 males which were selectively crossed (this qualifies as an open pollination). Females were assigned letters and males numbers. ‘A5’ is the cross of the first female and the fifth male.
There’s probably some variance in the germination numbers based on the males used but for first approximations, I’ll assume the germination rates are a reflection of the female phenotype.
This was an interesting and productive run.
I’ve made a number of notes to improve for the next project and I have a complete library of each of the 15 crosses that I plan on sending to Swe-can.
…
The next project will be a Co-op run titled OG’Er Encore …
the genealogy is Master Kush x Bubba Kush 
