Tissue Culture expert needed! Save rare 1970 Clone dying FAST!

Sex has nothing to do with tissue culture. Cannabis is totipotent so you can theoretically grow an identical plant from a single cell. Theoretically and regarrding

Again, there is no resurrecting. If the plant is dead its dead forever. Remediation is making a sick plant healthy again.

If it’s still alive then get it healthy and root cuts. I’m happy to help and can talk cost privately.

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I mean before it got to this point they should have shared the cut as a backup. Let me give you an example,I had a cut that I liked, I shared it instead of hoarding it. I lost the cut to a disease, it died,it didn’t root or whatever, the person I gave it to still has it (if they didn’t lose or toss it). I get it back problem solved( again with a trustworthy, dependable grower). That thing doesn’t look like a weed plant at this point. Try giving it some beneficial bacteria like recharge. Might help it with stress. Good luck

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@grief college, your thinking too far. i just got the only viable cut left. talk to the preowners.

thanks, ill write you tomorrow

Second this. Recharge or microbe tea of some sort. 24hr light like I said before.

Tea, yes. Microbes, probably not.

If you want a clean culture I’d rather see someone bring it back to life without microbes that may be endophytic and mess up cultures with contamination.

I see this with bacteria and with fungus both. They may have a symbiotic relationship growing through their normal life cycle but F up trying to grow an explant in culture when it all leaks out of a plant material.

I have some I am fighting through at the moment that are leaking pink bacteria 3+ weeks after being in culture and I DO NOT have rooted plants growing so I can re-culture then into antibacterial media.

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Has that cutting been taken from a flowering old plant ? Because sometimes old plants or some plants in genral will go through issues to reveg an some will never properly fully go back into veg. I would have also put it in clean soil to give it a better chance an in that situation (were it looks to be taken from a plant well into bloom) I would keep it under 24 hour lighting. The soil has all sorts in it, I can even see a feather, not ideal is it. Along as it has a couple of leaves that are green there is allways a chance of survival an of new shoots suddenly shooting out. With very old or inbred or cuts taken from old flowering plants this can sometimes take many months an sometimes longer. Might be a idea to spray it once or twice with a very light dose of hormones (auxin).

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I agree. Is it possible that it’s been in the same pot for a long time? If so and the roots are old it always a good practice to take it out and trim the rootball back some and get it into good fresh soil if you think it would survive the stress.

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yep, got a response. no leafes left he said… lol
he said he will snd pic today…

so from what i learned here , well… not good.

Now he told me he took her out the pot cause she was straight dying.

Now he gives her kelp aloe and natural root promoters to this dying Stem. LOL
he still didnt manage to just sen me a pic, its getting :smiley:

but who knows probably it will grow. But from what i know, as soon as there is even a millimeter tiny healthy Leaf, thats a big Difference to no lifing leave. i know there is less choices if its straight dying off.

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here thats how it currently looks:

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so, no leaves, so : very hard situation.
Stem looks astonishingly green.

I thought : why not graft a other genetic on top, cause with no leaves is there even a theoretical , scientifical possibility this can live again. i dont see even a tiny millimeter of leave…

grafting?

So, after the graft it may grow bigger, and one day, we graft this double-genetic, yet another time on on a different rootstock… once thats done we cut the highest graft so this 70s-branch can shoot new branches.

Is this grower trying to kill it or save it, now I’m confused :confused:

Stick it in a light mix, under a vented dome, and leave it alone please this hurts stop the torture :broken_heart:

A stick would grow a branch given some time and care… chances are slim but worth a try

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be assured I want to save it. what he does, i cant tell.

First primary question is: is there even a scientifical possibility this regrows once every leave gone… Thats a very important question. And the grafting idea , i think its the best.

If the branch isn’t dry and brown, it’s most likely alive. New leaves will grow out of what used to be nodes. I’ve saved plants that looked worse before :wink:

Now grafting could work but even a skilled hand won’t have 100% take. With only one shot at this I’m skeptical…

Long live the branch!

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so, you got a branch to live that had not even a millimeter leave on it?

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A beaten old stick of a weed plant. After a month, new growth came out of a few nodes

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@Mithridate didnt it have tiny tiny leaves left on the stick?
in my experience, i had young plants eaten by snails, and deer, and always if there were this tiny millimeter small leaflet it regrew, but the times there were very precise Snails cleaning it all up, lol then it died-