Trehalose what is it? How does it effect plant growth? How does it help with stress such as salt, drought or freezing?

@shag poor timing for me to do more than look right now. I’m thinking at some point do this in my 3x3, or somethinglike it. Attempt to anyways. I need to get back where it’s a bit smoother sailing before I change up much. Similar to our nutrient chat. “Going pretty darn good. Maybe change later, or maybe not.” I’ve been feeling good about pulling off a couple low stress grows. On me that is. Plants too. Still using the Lucas program you steered me towards what…2 years now? About. Normal add ons, in moderation. Hygrozyme, gypsum, molasses, CaliMagic, Kool Bloom. Some juice a mad scientist shared with me. Need to figure out how to get more. Was looking at BudBusterPro too.

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If you did start to use it, you would just use an ultra low dose spray for a slight boost in health and protection.
But I agree, wait until your bored to add it to the routine, you do not need this product, it is just an add on.

Just send me your address… :slightly_smiling_face:

I have heard good things about it.
But mine will cost you less. :money_mouth_face:

I have heard there are better choices for enzymes.
Also fresh is very important so the less commercial the better.

I will bottle up some juice for you and give you a shout when it is ready for ya. :heart_eyes:

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I have researched how anti-virals work.
They don’t seem to kill a virus, you can not kill something that is not alive.
What they seem to do in the case of covid type viruses anyway is blunt the spikes or protrusions making it harder for the virus to attach therefore it is not affecting as many cells or replicating.
The virus is being suppressed.

I feel that is what we are seeing in @tamimes experiments.
The virus is still present but is not spreading or affecting very many cells.

Think of Magic Johnson as an example, he still has the virus but there really is not any sign of it, and it does not seem to affect his health much.
The virus is suppressed.

Much love to my man @tamimes for the work he has put into this project and to come here and show and tell everyone is awesome and I thank you greatly:heart_eyes: :smiling_face_with_three_hearts: :heart_eyes: :smiling_face_with_three_hearts:
We had the science papers that tell us it should work, now we have a real world account that proves it does in fact work.

I find it funny that 2 stoners on opposite ends of the earth have come up with a valid way to suppress a virus that is running rampant throughout the world and all we get are crickets… :thinking:

You folks are hard to impress.
This is a tough crowd for sure.

I am no lab guy, but if I were a betting man, my money is on the fact that if you suppress the virus properly and for long enough like they do in tissue culture, you may just be able to take the very top cutting of the plant and get it test virus free without tissue culture.

To me that would be big news, but we will probably only get a image

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How much does it cost to cure HLVD, then? :stuck_out_tongue:

In all seriousness: it’s an interesting hypothesis. I don’t think we know conclusively that trehalose does anything to suppress HLVD though, rather than this simply being the presentation of the latent viroid before it’s activated. You’d need to find someone who’s actively having a HLVD outbreak to do that kind of testing, and then a lot of PCR tests to verify whether it actually worked or just made it latent again by reducing the viral load. Then you’d need to have a control group being run under the exact same conditions minus the trehalose, which it doesn’t seem we have here. Then, finally, a test to prove that clones cut after long treatment actually come out virus-free and don’t develop it later. I’m not sure we’re capable of proving it here; I know I certainly don’t have the equipment.

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$180,000.00 :rofl:

Nope, not specifically.
But we do have studies that show conclusively that Trehalose works as an anti-viral.
So to assume it is effective on HLVD or any other cannabis virus should not be too much of a stretch in my opinion.
In other words it is safe to assume it probably works on HLVD.
Unless you see a scientific reason to believe it should not, I personally see no reason to doubt its effectiveness.

I am pretty sure @tamimes describes perfectly his conditions, I would say that qualifies, did you miss that part or do you disagree with my assessment?
And yes all the lab tests and such would be needed to publish a scientific paper but I do not intend to do such a thing.

My proposed action was described above.

This is something that any of us could do for the cost of 1 or 2 tests, hopefully anyhow. :slightly_smiling_face:

@Cormoran
For reference to my statement above.

I should’ve been more specific. They need to not only be having an HLVD outbreak, but we need to actually see the dudding reversed or get viroid levels to know this is anything other than plants infected with a latent viroid that hasn’t manifested itself yet. As far as I know, it manifests due to stress, and taking excellent care of the plants like feeding them trehalose and chitosan oligosaccharides and all this might simply be suppressing the manifestation. In other words, more testing. :stuck_out_tongue: It’s a neat hypothesis otherwise, but not entirely conclusive. Weren’t the plants that showed signs of dudding still showing those signs?

So to be clear here about what I am proposing.

Treat the infected plant(s) with multiple types of anti-viral compounds until there are no visible signs of the virus.
Take a test.
If the test is still positive, treat the plant again and possible increase the strength of the treatment.
Repeat this until test plant dies from over treatment, back off those numbers just enough to keep the plant heathy and there we will find those magic numbers.

Once a good strong effective solution has been established treat a fresh set of plants with that for a to be determined duration and take the smallest cutting possible.

I also propose some brassinolide to make the growing tip go into overdrive thus increasing the tips growth dramatically hoping to so called “out run the virus”

After all of that, have her tested again and see if she is still positive.
I have heard stories of some folks cuttin the top tip and not having it pass on the virus even without anti-viral treatments.
So I am optimistic, but maybe someone will try it and then we will see for ourselves.

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What I was trying to say here was…
If I had a spray on the market today, and it was proven to allow you to take your virus infected plants to harvest without loss of yeild or quality, I think it would be a best seller.
If it were in a bottle folks would be talking about it everywhere.
People love the bottles, commercial ag guys shake their heads at that. :thinking:

Someone will read this and steal the idea and put it in a bottle.
Who knows maybe they are perfecting it right now as we speak. :rofl:

@tamimes and I are trying to give it away for free.
I wonder what folks would pay for a bottle of this?
What would it be worth to you? :thinking:

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I agree, like magic, he is not cured as far as I know, just not manifesting.
And yes this would be just a suppression on the virus manifesting itself.

Keep the sleeping virus sleeping.

And to touch on this a bit.
No, I do not think it could reverse damage done already but I do think it may stop the damage in its tracks.

It could do this by limiting or stopping the spread of the virus to other cells.
If it can not infect other cells it can not continue to replicate itself.
It will in hope be forced to sit there sleeping.
And yes, it could still pass the virus on.

Prior to anti-viral treatment yes, I do not believe so after treatment.
But @tamimes should answer that, anything I say is just an interpretation on his findings, so I would hate to misquote here.

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@shag you discribe it perfectly my english is not that good to explain everything in details🥰
@Cormoran im testing to save my system of growing first!! That is high density perpetual sog in non stop reusable medium (mapito) and also i have only 80cm total hight and 1 mtr total hight!!! And 2x diy 300w led
In this system im reaching easy 4 to 5 gram /watt on yearly bases ( im harvesting 26 times a year)
That i need to save first!!!
By save i mean( shag describes it perfectly) HOW bring my production back to normal even Having full blown hplvd in my system!!! I grow perpetue in a 1 tank recirculating system . So 1 plant hplvd all will have it!!!
How do you save that?? Everybody will throw away everything and start fresh and new!! I can not disinfect between grows because i have 5 sets of different bloom stages in my cabinet
So the last pics that i posted are from my nex to harvest plants and then i have 4 more sets in the system
I think i foud a way to out run hplvd!! For my system
see previous postings

Now comming back on your point about reversing or stopping hplvd that was not my priority but i have news im having 2 sets of clones of my old infected motherplants that got the same spray and are in flower
Pics will follow
I took a new cutting from all after the spraying and before putting in bloom😉
To keep your eyes happy i have some pics of exactly what you want😘




This is a pic of this morning of my best ril gorilla bubble can you see that it is hplvd infected( hope we agree)
This clone is also sprayed you can see the difference in leaves the top is bigger and looks healthier
I will clone the top and do the same prosses and see by time if im able to surpress the hplvd in the plants but my system must be solved first if not it wont work(hope we agree there)
I will put this plant in flower after topping so the lower horrible hplvd branches need to produce :wink: will post my findings

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This is her 3 plants in 1 pot

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@shag i also was thinking obout the brass or something else​:joy::heart_eyes:
Yes we should bring a fancy spraybottle on the market😋

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Looks like a plant to me, we’re definitely in agreement on that much. :stuck_out_tongue: As far as seeing a latent viroid infection, nope - though I might not necessarily know what I’m looking at, if these are supposed to be signs of dudding. Glad it’s growing well.

This is what I was responding to in the first place. I’m not saying it wasn’t a promising test, but taking a victory lap and declaring that you’ve got a system to cure HPLVD is a bit premature. I understand that wasn’t your goal, and I’m happy your plants are in better condition now. Do more testing - lots more testing - and maybe you’ll get more than a “meh” on the claim to have cured a latent viroid that can apparently evade detection for years. For that matter, have you actually tested that these plants had HPLVD before starting? I don’t recall seeing test results anywhere.

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No victory lap Im not curing hplvd ! Never ever mentioned it
You mentioned it that you wanted to see that how a sick plant can be cured
Shag mentioned botteling as a kind of joke
Im trying to produce normal even with full blown hplvd hope you understand me
And you dont see the hplvd in my ril plant?
Wow than i think you dont have much info on hplvd
This hplvd is horribel its like having aids and corona together

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This ril plant is horribly hplvd all syptoms there so it wont produce shit!! By flowering it i will have a total loss in produce
Try to explain
What you can see is by spraying it the top part is behaving better than the badly infected bottom
Now im going to do 2 things
Clone the top! Why because i think the viroid count is lower(plant out running the viroid)
And next flowering the most infected part of the plant an by spraying it ect ect trying to let it produce almost 100% of what it normaly does without hplvd
That is a victory!! So that you dont have a total loss!
Im not curing anything, no lab results nothing, not inventing the wheel im looking at my end product did i managed to harvest a top product(even having hplvd)
Just SHARING my finding, everybody must know what he or she do with it

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There may have been some misunderstanding here.
I do not feel either of us suggested we have a cure for HPLVD.
Sorry to mislead you here.
As you would say…What we are taking a victory lap over is the suppression of the virus well enough to harvest a crop without yield loss or quality loss.

Those are the 2 top complaints folks make when they have a virus.
If the virus did not reduce yields and quality or affect the health of the plant, no one would care at all.
I just figured since we seem to be able to avoid the 2 biggest complaints folks have when they get infected by the virus it would be big news.

Much like magic johnson, he has aids but he does not seem to worry about it.
The world thought that was really big news.
Much like magic’s big news, I thought this was pretty big news myself.
But maybe I am just easily impressed… :smiling_face_with_three_hearts:

@tamimes maybe we should go to market???
We can call it Magic’s Mist! Anti-viral plant spray. :rofl:

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And really a victory lap is probably not the best choice of words.
This is still a work in progress, we are just sharing our progress with the world.
On another note…
Ningnanmycin may be another thing we could to add to the mix here.

Ningnanmycin is currently the most successful anti-plant virus agent.
Some other anti-viral agents.

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Wow that is some new info more stuf to read😜

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About the magicmist set it up an i provide the proof​:rofl::rofl: when you buy expensive hydro stuff you never get proof or pictures​:thinking:

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