I am a bit uneducated with regard to what I should call my latest creation and I’m hopeful the OG breeding community can help. Here’s what I did:
Reversed a Peyote Purple plant and collected pollen (2021).
Made Peyote Purple S1 seeds with that pollen (2021).
Dusted my favorite S1 with the pollen from 2021( just now).
Two questions:
What is the correct terminology to describe the seeds this will produce?
Any bets on if that old ass pollen is any good? It’s been kept in a thermos with dry rice in a freezer that’s seldom opened since 2021. The pollen itself was stored in a tiny parchment paper envelopes inside coin envelopes.
I think S2 would be if he selfed a S1 to pollinate? If he used the parent fem pollen in a way it is a BX. There gotta be someone who’s more familiar with proper notation
I just exhaled loudly realizing I was 14 years old when you started this project! It’s almost half my age but hey that’s the right amount of time and work to put in right?
You’ve back crossed an S1 to the plant selected in 2021.
Thanks for sharing that pollen saving method - I have attempted to save pollen in the past and with a freezer involved had very little luck - I don’t save pollen or use saved pollen for this reason. I am very interested to know if your seeds set. Please keep us posted.
If this pollination produces seed I’ll be swimming in Peyote Purple seeds and will probably pair the remaining envelope (one left) with a suitable mate or mates. Use it or lose it, right? I have a feeling it won’t stay viable for too much longer.
The seeds are initially S1-F2-BX1 then.
Then are becoming S2-F3-BX2.
Not much will understand the filial notation tagging it “S2” is enough.
Viability depend mostly on the preparation of the pollen and its dryness.
3 years in the freezer without getting out is not a big deal.
It stay a S2. It’s incremential with the number of occurences of the same donor on itself.
Just like a BX.
Selfing is strictly a BX, technically ^^
The S notations is only there to identify a certain type of donor during the process : a specimen on itself.
There is a bunch of way to skin a cat, but without talking about sick methods and all … the most important is the drying of the male’s flowers and their pollen. Just s dry than you can, simple.
What matter the most also just after the drying is the RH during the day you’re packaging the pollen. Under by example 70% RH during a rainy day … it can be quickly an hell.
The best still fresh pollen straight from the male directly to the female’s flowers, but storing at least one reliable emergency pollen is always a good idea.
Sorry for this bad new. Train your method to dry heavenly the pollen, and only this.
Even with mistakes on storage etc … it will give sustainable results with the learning curve it’s implying.
Nop, the preparation of the pollen was obviously more compliant with a fridge context, with a max 1 to 2 weeks storage.
Maybe the grains were too moist at the point to explode by freezing … as well ^^
Thanks - do you suppose then to assume a BX will initiate a progression in its filial generation.
S2 BX
Regarding pollen - I find in most cases the challenging part is the temperature change when something comes in and out of the freezer. This transitional period creates ideal conditions for humidity to attack, with potential to nuke any viable pollen left over the entire preservation processes. I honestly have never given it the effort - I have revegged males on rare occasions - but find no need for saved pollen in my current project structure.
(home) Freezers is absolutely a single use, no backward. It force to package individually or only store massive pollination. If you can get rid of this constraint, hell enjoy ^^
So yeah the filial generation anyway is incremented each time. It’s very helpful to keep track of this, a BX1 F2 don’t fight the same against you than a BX1 F6 by example ^^
Any tactical step (“S”,“BX”,“IBL”…) is incrementing the F stage, and is changing then the relationship of dominances.
From the F1 to the F10 by example, a line will follow different patterns of segregation. These patterns are following.a model introduced by Mendel, with notions of dominances and recessivity over multiple inbred generations.
For the example of the BX, it’s choosing the optimal ground.
If you start a BX with a F2 generation that just start to segregate from the heterosis genetically … it’s going to be a ride.
But if you prepare a line for this, let’s say 5 generations, it suddenly become a breeze in comparison.
It’s not even related to a supposed recessive or dominant IBL, but more about the dynamics having the lead on the genotype at a given generation. The more groovy is the outcross, the less accurate is the BX.
