Cannabis microscopy


I got a few amber trichomes there, probably getting to the last week or so of productivity. If I do a good job on this study, I can probably predict when harvest day is based on the look of the trichomes. I mean, predict it a couple weeks in advance. They all probably undergo the same lifecycle of the trichome…yo! there’s my title! “The lifecycle of the Trichome” - a descent into the forest of delights. hah hah!

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hmm looks like I was extra violent taking that bud sample and ruined lots of the trichomes!

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ok here we go! Some meat breath day uhh 21! I am really filling in the progression of images now. I can’t wait until I take an image of the same day but months later!


These are bud leaf photos! You can see the progression of the trichomes is the same on the lower buds, and leaves as it is on the buds themselves. Just less trichomes per micrometer.
Woah heh heh I measured the same trichome at 4x objective and 10x it was only off by a single micron, guess I finally got the measuring calibrated accurately lol!

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That is a great title! :sunglasses: :+1:
and it goes well with your photos. :thinking: You know…

You might want to think about it, that might be worth ‘shopping around’ to publishers…

Cheers
G

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The crazy part is, with all these photos I’m getting more questions. For example:
What would happen to trichome development if I hit the plant with nutrient lockout?
What would happen if I put the plant into hibernation with cold?
ahhhh!!! Oh well any good research doesn’t answer a single question without raising 20 more…
Thanks for the interest in this project you know, it’s strange but I don’t think I would have made this progress without you. Your multitudes of questions are inspiring!
I think that’s the reason people don’t do good work in a vacuum without others input. It allows you to feel comfortable with your conclusions and ideas, without questioning. Questions, are what the quest for knowledge is based on!
Hah hah the dark side is when people don’t like questions. That means the quest for knowledge is already over. These images are a baseline for any future knowledge extraction from trichome development. Juicy data!!! Knowledge worth it’s weight in gold! :wink:

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WORD!

Exactly the conclusion I came to about my work.

Being on OG has forced me to up my game. Inputs from multiple sources keep me thinking and reevaluating conclusions.

Cheers
G

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Have you ever looked to see if hydrogen peroxide has any effect on spider mites and their eggs? I want to know if it’s a viable solution for dipping whole clones/cutting and at what strength.

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I used peroxide at 3.3% strength and it has a negative effect on the plants resins and waxes. Spider mites seemed to enjoy it. Sorry I don’t have photos of that, I wish I had documented those studies. Documentation was non existent at that point because I didn’t think anyone would…want to know. I just needed to find a solution… for myself. I recommend drowning them with water submerging for 30 min and then washing them off with the sprayer. It was the most effective solution I could come up with. Required no trips to the store and I could complete the task in a weekend(30min basically), and never see the mites again. That’s how I defeated an infestation of super mites that I couldn’t poison to death. Found out poison was useless as a solution to mites, so I changed it up to never use poison.

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Submerge for 30min, got it! Thank you! Prob a once over with a tooth brush prob wouldn’t hurt.

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lol yah I use the vegetable sprayer to give them a quick rinse after being submerged. I didn’t get to test if you have to do that because the mites were all killed off, so there were no do-overs. uhh which was a good thing! Now I just keep doing the same thing because rinsing it off after submerging it is not a complex task. Tell me how the toothbrush thing works! I’m always interested in other stories.

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Will do! Also wondering about sonic cleaning a cutting… I’ll test a small cut to see if a jewelry cleaner will damage it. I think it may rupture some cells or tissue, only one way to find out! Gotta dig mine out and see if it still works.

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ok I got some images!


The final one I used 40X objective so it’s huge. I knocked a few trichome heads off but hey, it’s all in a days work??

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Something I don’t often do is measure the width of the stalk. I’ll get a few images like that in as well. I’m curious as to how that varies. oh! shit! that last image had huge artifacts. Didn’t notice that, what a crappy image.

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The purple going up the stalk of the trichome surprised me somewhat, I’ll say. The plant itself isn’t purple so what the fuuuuuuuck is it doing there. I mean, are they all like that?? huh! I might have to just study that part of it hahah! Naw but in reality, we’ll see what happens. Got to get some more imaging done on other plants. All shall be revealed in time, young grasshopper.

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here’s the king tut at the same day. Well fuck me no purple. Case closed. lol lemme get closer, I see something.

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There’s some kind of color at the base of this trichome.
Also, check out the caps, they are practically identical to the big bud ones in terms of width.

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Phew! Feels kind of funky like cortisol levels are through the roof! This is the kind of day I need some powerful medication. Brains trapped in jello ooof. I had some kind of late night glucose thing, always sets me up for a shitty day hah hah! OK enough of that.
I took a sample of the meat breath. Here’s the strange part. I don’t see any…thing, to sample eh? There’s a tiny nub in between the branches. There’s leaves and those spiky things. I am going to check a part of a leaf that is really close to where the buds will start. I’m going to have to go in there and look around on all the plants. See if I can determine where a good sample zone is.
I know before I said I would sample a pre-flower yo…there isn’t one? laughs better rethink that one.

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I measured things. Small things. Leaf things! When the lights come on I’ll image a different sample.
You figure the trichomes are coming out of where those spheres would? Expands from where a sessile trichome would be?

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check this out!


big bud!

meat breath!
I assumed, as it turns out, incorrectly, there would be something to sample like on the big bud.
So, I’m going to go image the blooming plants. I got another idea.


ok…ok ok! obviously I’m looking at two completely different plants. They have almost identical structures in the end, but it appears to not start out that way. Interesting!

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