Hormone and sex manipulation on cuttings, past research on hemp

Advanced Techniques
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I’m not sure it will help much but on treatment for polyploids, the waste is pretty large. Both in non-viable expression and herms (true ones, seeded from the core directly). To put an average number, i’m talking about over 75% losses.

Not sure either on the pertinence but actually PGRs treatments (an heterism for me) can maybe lead to have a good base to set the rates for the first experimentation. This shit is plutonium, i guess it have to be drived by Mol to leave the “flowering booster” state without reaching either the hard mutagen it can be when used for genetic generation.

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There is not a lot of info out there stating how to use hormones to make buds more dense.
What hormones are you refering to?
Paclobutrazol comes to mind when you say this, as it is a gibberelin inhibitor.
While Paclo does work well for this it does reduce trichome production.
Then we should also talk about the safety concerns of using this product.
So to summarize a bit here, Gibb. will not make buds more dense but it does make them fluffier, and it will increase overall mass/weight of the flowers.

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Plutonium. Appropriate visualization. :wink:

I did try methyl jasmonate at one point but the branches it was applied to completely wigged out.

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That’s funny tells me that tropicals have higher gib content maybe and it depends on the interactions of the roots so to speak. Maybe different root structures dictate more things than we think?

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That makes good sense, they are usually on the fluffy side.

Cytokinin made in the roots can mediate/counteract the effects of Gibb.

You may be on to something here, :exploding_head: we may need to dig deeper to know for sure.

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Contact with hormones, in general, are things to be cautious about. The amounts to elicit response is exceedingly small though which may make it viable for what you’re thinking and relatively safe. Not sure of the methods you’re considering. Topical or what not. Also, looking at SDS on some plant hormones, there isn’t a lot I’ve seen regarding inhalation pathway. Nor have I seen longer term physiological safety information … although I have not really looked around so much. I do believe it is really really easy to overdo the application.

Though, the topic here is primarily exploring whether manipulation could result in reversal and in a productive manner. Earlier in the life cycle, does it revert, is it useful. idk.

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Well you have that choice and that’s useful?

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This is true but some private research has been done.
You used to be able to find info here
https://manicbotanix.com/latest-cannabis-cultivation-research/

Some other work was done by Spurr and some consutlation was done by Only Onamental as well.
These folks were involved with the whistleblower case against Bushmaster ect.
Most of these phyto hormones are already found in the plant, the ratio is what gets changed causing a change in growth.

If you are using Bud Buster Pro you are more than likely using some of these phyto-hormones.

I am not claiming that as absolute, I do not posess the fomula.
But the question I would have would be…“How do you get that reaction from plants without effecting the hormone ratio?”

We also found out that the more natural the hormone the less chances of interaction within the human body.

That OO guy was pretty good at organic chemistry and we discussed the potential harm that could come from using these products and while he does not think these products are the best choice he did give a green light.
Of course proper and thorough testing needs to be done.

Without checking my notes I remember that NAA was one to watch.
Something about the chemical when combusted.
I think it was CCC that turns into a cyanide gas, or hydrogen sulfide…it has been a while and I would need to check my notes.

A bit of the background here…
I am just standing on the backs of the giants that came before me.
I learned of hormones on my own but his is where it really started for me.

That and https://manicbotanix.com/latest-cannabis-cultivation-research/
The guy G. Low was working with OO to make a pharmacudical grade medical Marijuana nutrient line.

They had a huge start on the project, I am not sure what happened?
Some funny stuff surrounds that guy G.Low, he was growing football sized buds with PGR’s then he went legal and became holier than thou and tried to bust everyone doing what he used to do.
Don’t get me wrong he did a bunch of good getting bushmaster taken off the shelves, but you can still buy paclo products if you want them.
My point is he did bring awareness to the sorta things.

For the record he was good with using certain hormones in flower but not late.
At least the products he developed had hormones in them.
They were not the phamacudical grade nutes he wanted to make but they were said to be specifically designed for medical cannabis.

So a lot of time and thought has gone into this hormone topic, I am not just shooting from the hip here.

FYI
Most of these hormones are approved for use in a lot of food crops.

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Choice on what?

What is being asked as ‘useful’ is whether there is an advantage to applying what the paper author’s have described. I could see some use cases if it works but maybe the advantages are/aren’t there.

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I think he is saying …“Being provided with the knowledge gives you the ability to make an informed decision and that is useful”
I am pretty sure that is what he is saying.

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Before this become circular and for the purposes of the side topic, I can open a separate topic on hormone application into flower if you’d like.

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You should ask my man @Cactus really, but do what you must.
The threads I start on advance topics like that usually don’t get much response,
I personally don’t have much to say about using hormones in flower.
But I am interested in the topic if you wish to discuss it further…

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Then, sex manipulation on cuttings via hormones. The paper’s authors have made note of using hormones to manipulate the sex of cuttings on hemp. Does it work in practice and would it be useful? For instance, could one force cuttings to reverse, force flower, and then extract enough pollen off the cuttings to self? How much effort to get there vs using some of the other techniques? If it works and with a reasonable amount of effort, there would be some advantages.

Hempy mentioned perhaps trying to run an experiment which would be interesting, in this regards.

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It’s there since a bit, a bit old too but the process is well described and can give some leads. Who have a gamma projector in his garage ? ^^

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Yes, and yes.

I agree, that is why we are having this discussion.
It is not like we just found a paper and we want to see what everyone else thinks.
While I have not done this personally it is proven science.
I even gave a in depth description of how it works.

Sorry if I ignored that statement, I hear folks say this all the time and the work is never done.
Not to take anything away from @hempy that has just been my experience these last 10 years.
He may very well come through… :slightly_smiling_face:

I would absolutly love to see folks doing these sort of experiments in public.
But as I have said before unless money is involved folks almost always shy away.

@Cactus is the only gent in the last 7 years to chat about these advanced topics with me and actually get something done, so hats off to him he is a good egg. :smiling_face_with_three_hearts:

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Well @shag most humble thank you. I think I might try something but will need some cooperation with the girls and boys to get bigger for I am a little behind in my gardening but things are moving along nicely.

Man gibberillens have to outgrow the competition, hmmm fast growth can cause problems yeah starting to hmmm

Much Love and Respect

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FFS. The amount of contradiction and self-pleasuring you engage in is next level. F’in toxic.

Actually, yes it is. That’s called a discussion.

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I won’t do any experiments for less than $10000

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I used GA for the first time this year to germinate some old seed and to be honest i wont use it again.

I ended up with males and females but i also found a hermaphrodite at sexing in the line i have never seen a hermaphrodite before.

I have never been interested in sex reversal using chemicals to reverse a flowering female to a male it is not something i would do.

Saying that if i can use a female clone and by simply cutting the clones roots off and placing that clone into flower and having that clone go from veg directly to a male with out it flowering as a female and then having to use chemicals to have it turn to a male this has my interests peeked.

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I saw the tri on the left Loki ^^ bad bad bad dog lol

update : Hempy is right to expand, let’s be less casual a bit for the sake of safety and realism.

Shitty disclaimer 1 : GA3 is not a magical tool, and have nothing to do with STS that i consider personally as a true industrial tool with predictable results.

In the “i use or i used”, there is a reality when you take it seriously and that look like it a bit :
yupyep

TC is more known now so it don’t look like fancy today, it don’t change the pain in the ass that is the process done with a responsible procedure and environment.

It took me two years to obtain reliable polyploids, and it’s not all about dosages. But also to understand the right inbred line to use for it, and to discover on the late that the said line need to be prepared for it in bonus.

It mean also a large space where almost nothing will be produced and where you will waste most of the specimens alive. Pure R&D ^^

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