Hormone and sex manipulation on cuttings, past research on hemp

For tissue culture and stuff. I haven’t used any of those on plants intended for consumption personally. Or, I should say, I’ve tried jasmonate once on a part of a plant for later use.

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This is the GA3 i used i got it from Israel from a seed saver group.

It did help with germination but using a formula of RO water and Hydrogen peroxide works as good i believe.

This is the stem elongation it causes.


Eventually they do grow out of it and go back to normal.

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I’m not a pillar of the GA3 game in anyway, but trust me the formula is well balanced with what you show. It took me a while to obtain a lot more fucked shapes than this ^^

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The last picture is of the two males.

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Not much will get it but the ones falled in this rabbit hole, bt too toasted to play the prof with a wall of text sorry




All gib polys, well the ones i’m not in shame to show.

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These are the two fems.

Flower from the one on the left but both were as encrusted in resin.

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I guess I must have brought that on myself at least according to you right…LOL Thats ok, I am used to folks throwing out insults round here.
Sorry you feel that way, but your perception is way off base.
I am just trying to help the community.

Actually, no it is not like we just read the paper.
If you read my post then would have seen this discussion has been going on for more than 10 years.
You may have just read the paper and this may be a new discussion for you but not for some of us.
We are just trying to share what we know, hoping we can teach each other something along the way.

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I agree the amount of Gibb looks like a good one.
I would say it is a very good well thought out product.
I have seen some plants grow like beanstalks and keep growing too.

I agree, unless you use too much on a small plant, then they use all their energy and die.

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Just cutting off the roots can turn a female into a male without any chemical addition.
This works by removing the plants ability to produce cytokinin.
From the paper linked above…
You can use BAP to get the plant to return to female if you wish.
This works by replacing the lost cytokinin therefore the plant returns to female.

@Cactus
We need to try cytokinin on some males much like your Gibb experiment on females.
This should help weed out all the intersex traits in the females if
we are correct.

Rules of thumb for those just learning about hormones.

  • Always read the MSDS/SDS before using any chemical.
  • Always wear proper PPE gloves eye protection ect.
  • Don’t eat or even keep a drink in the room when you mix or add hormones.
  • If you get any on your skin/hands, wash it off immediately.
    Know and understand your product before you use it.
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Sorry really lit up and reading your stuff, man making sense man! Yeah when I land I will read more of this but man this weed is funny in how it changes your thinking and comprehending, man they are in two different places.

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I’ve a bunch of F2 (~30) on the go (germinating) i don’t care at all, just seeds-to-smoke. Not a big deal for me to keep one alive instead culling it because i don’t like its face.

Then cutting the roots and show you what the fck happend. I just need a process or i will do it my way.
Zero chem, just to see if it’s like boiling the root to have more potent weed ^^

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Yep so I think I better keep my witts about me and use the proper lab gear but I have worked in labs before so just same old same old. I like the organic stuff and how you can isolate stuff, isolated caffeine out of regular coffee, solid caramel lump of caffeine.

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May I suggest you check out the paper for the scientific approach.
But a real short answer is to cut off any new growing tips, you leave some roots but not the new growth.
I think it is best to check em everyday.
Maybe a magnifying glass would help.

The idea is…
Cytokinin is made in the new growth root tips, if you remove the cytokinin you remove the the female hormone and put the balance in favor of male.
If I read everything correctly this would change the plant to male even if it was a true un -reversable female.
In other words it has nothing to do with intersex traits, to the best of my knowledge anyhow.

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I barely see it like making a cut but filled with GA3, the difference is that the whole plant is used instead a bit of it lol Damn no i can’t reproduce the thing here, i’ve litterally to stop to grow everything else.

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As to sex expression, GA3 treatment resulted in
the formation of more than 80% male plants, while
in the controls the percentage was ca. 30.

Looks like after gibb treatment of 25 mg/l produce 7% females that were resistant to the gibb treatment. That I wonder is a good indicator of inter sex resistance?

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Just to be clear here…
What my man @Cactus is thinking is…

You know those females you just can’t get to reverse, you know the real rare plants that never ever show intersex traits.
Well what he has proposed with the Gibb would allow us to find those plants much faster than our current methods.
At least faster than any method anyone is talking about in public anyway…LOL

I would have to say yes, but some trials are in order to know for sure.
7 out of 100 is about what I would expect to find.
Plants with no intersex traits at all are rare, at least to the best of my knowledge.
@Mithridate @HolyAngel
Do you folks have any imput?

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Yes but then you are selecting plants based on how much natural femming cytokinin, auxin or ethylene levels they have not exactly if they were all gona throw bananas. So you lose diversity maybe gain short branchy squat plants.

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So by removing the roots on a vegging female clone you dont have the female hormones along with the male hormones you would from a reversed female all ready flowering.

I would do this with a fresh clone taken in veg and that way i still have the female in veg.

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Well looking at the plant heights the females are a little shorter but isn’t that the case anyway that the males are narrower and taller so I think that’s what is happening just the inter-sex and males are brought forward and then depending on your goals make selections. The heights are listed on the sheet not sure if you saw that?

These other hormones have have other applications too. So many things to work with just a matter of your imagination.

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This is very interesting……

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