But doing this with just one donor and one recipient doesn’t make it acceptably stable in the breeding community, correct? Wouldn’t they call this more something like F10 S1 or something?
Because there could still be variations, right?
2 Likes
It’s that easy, at least to describe the process. 
The results will depend on the particular genetics of each female, which is building off the work of the people who brought it to F9 in the first place.
I think this is one of the misconceptions about inbred lines. It’s not like hitting a switch. There are degrees of inbreeding - the more inbred, the less variation. F- and S- gens aren’t inherently better or worse, just ways of denoting what’s happening… F10 means it’s the 10th filial generation, between two siblings. S1 means it’s the first self-pollinated generation, with exactly the same genetics shared between the parents.
4 Likes
I think that for your naming and selection process that would be accurate. Commercially and official scientifically I’m not sure, but the logic path is there.
Perhaps commercially they F-x to a desired plant, then you start your ibl selfing…I am zero expert however. I’m curious too!
2 Likes
Ok, I’m just going to share my current plant for the future. This will hopefully give a look into my mindset atm.
After this auto grow, that’s about to be underway, I plan to attempt my first run at collecting and distributing pollen. The idea is likely just to reverse and pollinate the same type of plant for a fairly stable supply of S1(?) seeds. Many of which I plan to gift here on OG(if they’ll have them, lol). Hell, maybe I’ll just try the isolated branch thing so the donor can still be viable. Idk.
I’m gonna preform said sex change operation on the Emerald Fire OG autoflower F4 or the Blueberry Muffin feminized F9. Both sourced from HSC. I’m not completely confident at the moment. So I think this is a good way to get my feet wet. Rather than attempting a cross. What does everyone think?
I’m open to all comments, suggestions, and criticisms.
2 Likes
So, S1 meaning a clone?
I kinda knew that an F9 IBL would have few genetic variations or phenotypes. Just wasn’t sure.
2 Likes
Sounds great. Grab some CS or STS, start a grow journal, and I’ll be there in line with my hand out for stabilized fem seeds. 
S1 would mean you reverse one of the fems, then pollinate that same plant - or a clone, either way - with its own pollen. It’s likely to be materially very similar to a F10, if it’s a stabilized line in the first place. IMO F10 is a bit better because there’s probably less of a chance to get the same negative recessive in both of them, but you could just as easily say S1 is better because you can’t end up with a negative recessive from only one parent that way; it’s all or nothing and you can narrow it down later. That also depends on the genetics of the F9s though, it’s a rabbithole. 
5 Likes
This whole endeavor seems like a rabbit hole.
Hence why I just can’t get enough of the information, lol.
And currently this discussion is keeping me out of a trim jail sentence, which needs to be finished serving. 
3 Likes
An S1 could have males aswell. If a male is selfed, the offspring would have 67% males and 33% females 
They all would be S1s 
Pz 
3 Likes
I just had a very successful first run with STS. Although, the conversion took about a week longer than I expected.
With 100ppm CS, I tried on several occasions and never was able to have success. Maybe with 250ppm CS, it could work. But I doubt I’ll try CS again.
I’ll now stick with STS.
6 Likes
Yeah CS will take effect when your plants are on the brink of death from silver poisoning
STS is way better 
Pz
5 Likes
Hi everyone! I saw this post and was drawn to participate. I started cannabis gardening in June of 2017. I was paranoid about purchasing seeds from overseas (I’m in the US) and finding seed banks in the US back in 2017 was not so easy. Plus I didn’t know at the time who was legitimate and would was going to rip me off. My good buddy in NY said he would share a few autoflower seeds with me to get me started. By this time I had already come across posts talking about reversing plants to make your own seeds and knew that was for me. He sent me 3 White Widow auto and 3 Train Wreck auto seeds and I decided to start with a Train Wreck seed. I had already ordered a bottle of reversing spray off the internet and had that in hand.
GUYS AND GALS! I’m here to tell you, quit procrastinating and make your own seeds.
I treated the very first plant I grew, that Train Wreck auto and made 80 S1 seeds and haven’t looked back.
I’ve been making seeds for myself ever since then and saved myself literally thousands of dollars doing so.
From my experience STS is the way to go. I used Colloidal Silver for several years and had very good results but the CS is more labor intensive. My buddy on here @GCBudz is about to start a seed run using STS to reverse his prized Chemdog plant. Maybe he can be convinced to journal the process step by step with photos?
I will say this, when looking to make S1 seeds of a photoperiodic plant the best method I have used is to take two cuttings off the same mother plant, get them rooted and a foot to 18 inches tall and flip them. Ten days prior to flipping them both spray one of the plant with the STS, then spray it again 3 days later, and once again 3 days later and then flip both plants into flowering the next day (day 10 of treatment) and you’re off. I treat with the STS right before lights out every 3rd day for a total of 9 applications. I use the same STS ingredients and formula that GCBudz will be using so an apples to apples comparison.
I do hope ya’ll give it a try. It’s a lot easier then you think to make the female pollen. The tough part is controlling said pollen so you don’t end up pollinating all your girls.
15 Likes
Cannabis plants when flowering produce a chemical called Ethylene.
Ethylene is key to sexual expression in cannabis with female flowers requiring much more ethylene to develop than male flowers. By applying ethylene-inhibiting agents such as STS (Sliver Thiosulphate) or Colloidal Silver we can induce male flowers on female plants to create feminised seeds.
2 Likes
It wouldn’t hurt to allways have a bunch of feminized seeds, some with a shorter flowering period.
I mostly like the 10+ week varieties. I have one space, and when they show the gender it leaves a blank space. Perhaps it could be filled with such feminized plants. Short veg and then flowering…
Currently I have Sugar Punch(Sannies) and C99(TRSco) in the beginning of grow. I thought male female cross, but now it seems to me that the fem option is better
Or should I read the forum less 
And one question
Is there any difference which plant will recive, and which will give pollen. Mitohondrical DNA…
Thanks
1 Like
This is fantastic guys, keep it coming! Much to be learnt, I’m definitely going to be giving it a go, my take away so far is to opt for sts and selection is key to future success coupled with timing. I’d love to hear more about your methods of application and selection processes
3 Likes
STS vs. CS:
4 Likes
Have you selfed a Male?
Do Tell? Never tried, but I have wondered how that might go. Pics?
3 Likes
Yeah, done it in double recessive projects 
Pz
2 Likes
What kinda buds do you get on a male Canna that has been chemically “encouraged” to trend Pistillate?
When in the male’s life cycle do you do the reversal?
PIX?
3 Likes
@GrouchyOldMan Alot fluffier buds then a female counter part. You get less seeds then female reversal. The chems you use on males are something that makes em thrive, not like CS or STS. So the best results I’ve got is by using low dose 2-3 weeks before flip and up it the same day you flip. This will follow the natural preflower to flowering cycle. You keep feeding the florel and switch mix through out the growth of the seeds.
Hope i could spark some ideas 
Pz
4 Likes
Thanks for starting this thread bro, set to watching. One question I have, is it best to work with a natural female or one from feminised seed?
1 Like