Hey that’s awesome to hear and yes for the most part the regular instructions should be followed. The main difference is that I found I needed to allow the leaf matter to sit in the extraction solvent longer than the suggested 2 min otherwise the colors are just barely visible. I’ve been waiting up to 10 minutes I think for the last tests that I posted. I also shake a few extra times during that period. I do think the two minute time frame works well for flowers though.
I found if the leaf is fully dried it’s easier to crush up into the test tube. But the test has still worked for me when the leaf was not fully dried even after the 50 min decarb @ 240°F. I think having the crushed leaves also allows better surface area coverage with the solvent.
Edit: I don’t measure the leaf matter any more, I have just been filling the 1.5ML tube to just under 3/4 of the way.
The only other tips I found that you may have already read about is that after the mobile phase is complete, let the plate dry pretty well before dipping it into the dye otherwise the colors can get splotchy.
Then after it’s been dipped in the dye allow that to dry pretty thoroughly and then scan it right away as you will notice after a couple days the results on the plate will run and degrade quite a bit, they are not really meant to be kept.
Excited to see your results and any other adjustments you might find for improvement. Good luck!
Edit: Forgot to ask if you ended up getting a Micropipette? After a few rounds I finally purchased one and feel silly for not getting it in the first place as it’s so much easier to work with. Apologies if I had previously neglected to mention that.