Trichome Microscopy 🔬 Harvest Timing Science

I’ve definitely tried to touch a trichome while under the scope!

The diameter of a 28 gauge needle (the tiny ones for insulin use) are about 3-5x larger than a trichome head!

If there was a way to inject something into the trichome cap, it would probably involve freezing the cap first because the cap “skin” is basically just semi-hardened resin and bursts/tears when damaged. Whatever is used to inject material into the cap would need to do it with precision as to not move around and tear the “skin” and just leave a tiny 5-10 micron hole, and also the added material would probably burst cap due to pressure too!

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-https://images2.imgbox.com/4c/39/w9VCAsdg_o.jpg

e.g. Burst cap

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What size magnifying glass?

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It was under a microscope. The zoom times objective calculates to 2X though that doesn’t seem right.

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GIF test, I don’t think it’s working (seems to hang mid-video). Is this working for anyone else?

Also just in case we have some mods in here or someone that knows the forum tricks … can I post an MP4 link here and have it auto-play?


-https://images2.imgbox.com/29/8d/FtsRa52l_o.gif

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Works for me but took a minute to fully load

Cool! It’s like flying above the trichs

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Thanks! OG has a 700kb limit on linked images like it’s 2003. The full resolution zoom videos are really great in 1080p, I may need to make YouTube account.

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That is pretty cool, thanks for the hard work.
If you need any photoshop stuff, just ask and see if I have what ya need.
I hold a lot of differnt things,

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-https://images2.imgbox.com/ef/33/JnPSSos2_o.jpg

Glassy. Dry trichome.


-https://images2.imgbox.com/73/c1/zfg7biQ3_o.jpg

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Amazing images mate! Thank you for sharing. This has become one of my favourite threads! :evergreen_tree::fire::raised_hands:

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-https://images2.imgbox.com/47/e4/ldYBcYH1_o.jpg

Stack testing continues. On this stem image I tried to use as few stops as possible while maintaining ok-ish quality. For some size context, that stem is 1.50mm in diameter. This was taken with a different lens than the trichome shots. Pretty cool to see that everything is covered in tiny waxy resinous fur.


-https://images2.imgbox.com/97/c8/aVLQZIqe_o.jpg

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The stem pic looked fuzzy in the preview I thought “that’s weird for him to post a blurry pic” :rofl:

Super neat all the textures there! How many stacks did you wind up with?

Beautiful trich shots too. Great work! The purple vein is sick :sunglasses:

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Thanks man! :grin: The stem pic is a Helicon Focus “Method B” merge of five images, each around 0.15mm apart.

Take the Red Vein pic for example; a seven image stack merged with Helicon 8.

To get those seven images, I shot seven 1080p videos, one for each focal distance stop.

For each of those seven videos I extract 32 consecutive frames to TIFF format (each is a noisy grainy mess), then register (align) them because things move under the scope quite a bit under the hot lights. Then I merge those 32 images to a single image in Fiji using the Z-Projection function.

Here’s a single raw video frame:

image

vs. Z-Projection of 32 of those frames:

image

vs. Final Output showing other merged frames, color correction, sharpening:

image

So that image is technically made from 7 sequences x 32 images = 224 raw images + 7 resulting z-projections = 231 images. :nerd_face:

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That a lot of work, thanks for sharing it all with us. :wink:

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What an EXCELLENT thread this is! As Jesse Pinkman might say: “Science!”

Thank you to all contributors, but especially to @LD50 for the time and talent (not to mention investment in equipment) on display here, and shared with the community for the edification of all.

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hmmm I wonder if extreme temps while growing has an effect on the terpene levels. This guy I know who used to grow flavorless weed always grew at like 30C.

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Some crazy science you got going on there @LD50
Absolutely beautiful pics…
Keep em coming💚

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-https://images2.imgbox.com/88/54/XBqL0mIC_o.jpg

Crystal Ball.

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-https://images2.imgbox.com/ba/5b/xQE3nOBH_o.jpg

@Heliosphear Hey there, are you planning to do calendar once again? I think I’ll try to get one of these trichome shots on there! I’ll be on the lookout for any information.

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