Used a 27G needle and around 100mg agno3 and corresponding amount thiosulfate from the original recipe (Khalifa Genetics)Plant is Fastbuds banana purple punch. Seems to be a lot of pollen in sacs which is now being tested on 7 other female AFs
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Excellent results! Iām about to try the very same thing myself using STS (as opposed to just AgNO ā).
Can I ask, where you made your injection? Was it into the main stem or somewhere else?
Cheers, Barry 
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Awesome, thatās a great result, going to be a lot of pollen on that plant!
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Yes, it took several āpokesā, initially hit the woody part of the main stem without success. So, moved up about halfway up main stem into a hollow area. It went in pretty easily and then squirted out through a few of the lower injection site attempt holes. Also, would add that the needle can become occluded with plant tissue from the insertion itself, so if you feel like you are in a hollow space but canāt get any flow, this can be the cause. Hope that helps
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Thanks for that info @Ben63. I just shot her up now and found some good hollows on the main stem, I also made some small injections at the nodes.
Now to play the waiting game and see if it was a success 
.
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I injected after the plant was already showing early female flowering. Nothing much happened at first, then the pistils disappeared and after about maybe 3 weeks, the male flowers appeared everywhere. Only downside is that there donāt seem to be any female flowers left for āselfingā, but oh well. Good luck!
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Thanks, thatās good to know. Also, did you only do the one application?
Iām surprised it took that long to turn (or am I being unrealistic), but timing isnāt too important anyway as I plan to pollinate a different plant and hopefully saving some of the pollen (if it is a success).
Anyway, Iām more in it for the learning experience really, seemed like a solid idea so had to be worth a try!
Cheers guys 
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Good to hear that it worked. I almost forgot that I wanted to try the silver nitrate. Iāll inject mine tomorrow
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That was my experience too. When I injected it seems to me it went down to the roots in the hollow stem, then was sent out to the entire plant. It did make a few seeds before all of its flowers were converted but not many.
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Yes, just the one injection as wanted to see if that was enough and also because slainās original premise was finding an easier way versus the repetitive spraying. I would say that it did not seem to stun the plant at all, but does seem to prolong the process. If one is going to pollinate other plants during the process rather than just save the pollen, might consider starting the recipients a little laterā¦
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I just stumbled upon this thread and im intrigued. So inject the silver nitrate solution into the mid to upper maim stem and one is trying to get the injection into the middle/hollow part of the stem correct?
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Thatās how I understood it too and I want to try. I got a bag of silver nitrate here.
If anyone wants to try too, I got enough to send some.
Just keep in mind Iām in OZ. If your customs are crazy like ours,expect them to open the letter. Iāll state what it is, theyāll test it and will send it to you after I guess. They do that with my fertiliser saltsš
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Exactly. As I mentioned, it took me a few attempts to get it right. Also , I used STS because I had it mixed up and was curiousā¦
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I was doing some other research and came across this from ChatGPT:
Another study has introduced a novel method for stimulating male flower production on female hemp plants using a commercially available floriculture product known as Chrysal ALESCOĀ®, which contains silver nitrate, similar to the active ingredient in STS but with improved shelf stability. This study compared the traditional STS method to Chrysal ALESCOĀ® in terms of male flower induction and pollen quality. Both treatments were applied to female hemp plants, and no significant difference was found in the number of male flowers produced. Interestingly, pollen from Chrysal ALESCOĀ®-treated plants showed a significantly higher viability rate compared to pollen from traditional STS-treated plants when assessed with fluorescein diacetate staining. This suggests that Chrysal ALESCOĀ® not only matches the efficacy of STS in male flower production but may also enhance pollen quality, offering a viable alternative with the advantages of better shelf stability and ease of applicationāā.
Looks like thatā¦ 
maybe 7 oz bottle is ~$136 
Unsure on dosage rate too
ah, here we go:
Each ethylene inhibitor treatment was prepared for a final 1.48 Ī¼M silver concentration using a modified protocol from [20]. The total solution volume was 4 L of STS prepared by mixing 0.5 g of silver nitrate and 2.5 g of silver thiosulfate into 1 L of water and thoroughly mixing by adding 3 L of water to make the final 4 L solution. Mixing is required, and the solution must be made freshly for each application to prevent precipitates from forming before treatment.
A total of 4 L of ALESCOĀ® solution was prepared by mixing 17.65 mL of the product with water to a final volume of 4 L.
Treatments were applied using a 1.5-L backpack sprayer (Uline H-7986 Backpack Pressure Sprayer; Pleasant Prairie, WI, USA), and individual plants were sprayed until runoff, or approximately 400 mL per plant [20].
Spraying was conducted by utilizing a modified protocol of three 1.48 Ī¼M silver concentration spray applications with a 5-day interval between each application once flowers were initiated [20].
The first treatment of STS and ALESCOĀ® was conducted on 3 December 2021, when flower initials had formed.
The remaining two applications occurred on 7 and 12 December 2021 [20].
Male flowers were counted after plants had reached floral maturity but before the dehiscing stage of flower development on 7 January 2022 (Figure 4). The effects of the two ethylene inhibitor treatments were compared by a 2-way ANOVA.
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Quick update. Just one week after injection and Iām already seeing a lot of male pods. I wasnāt expecting things to move so quickly, but pleasantly surprised.
I think I can confidently call it a complete reversal. Now to see if they will produce pollen and, if so, will it be viable?
Itās all pretty exciting 
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Interesting! Thanks for sharing.
I found it for $109. Still quite expensive. But at roughly 4.4ml per liter a 200ml bottle should make up a decent number of batches.
https://www.pestrong.com/1913-alesco-anti-ethylene-treatment-200-ml-676-oz.html
Looks like the bottle has a shelf life of 18 months, and once you mix it into water they say to use it within 3 days (so youād want to only mix up what you planned to use, obviously)
I wonder if itās safer to spray than STS? (not that STS is that unsafe or anything)
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Nice!
I might have to give this a try. What dose of STS did you give?
Very interesting! I love these alternative ideas. May have to experiment with injecting the next home cross that I decide to cull.
Anyone have thoughts on whether injecting with Chrysal ALESCOĀ® would be different?
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It seems to be pretty similar to the STS really 
Just reportedly higher pollen viability with spraying Alesco vs spraying STS. Iād imagine itd work even better with injection than STS does. I donāt see why it wouldnāt. I need to try the injection tek on the TK and SSDD sometimeā¦
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Hi TeddyNuggets,
I just followed @Ben63 's lead and used the same STS that I would normally use to spray. My mix is the same as the one he listed in his post, I think itās a pretty āstandardā dilution 
.
Same as Ben63 I used a 27G 1mL needle and injected the majority into the main stem. I also chose to make āsmallā injections of the STS to the nodes.
One off application at day 21 (autoflower) just as early pre-flowers were forming.
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