Hormone and sex manipulation on cuttings, past research on hemp

In detail, it’s about pistils-less females (last update). They are unable to be pollinated, but technically not sterile. Breeding sterility with cannabis is quite difficult anyway until we are able to identify early (first leaves set) and rate the hermaphrodism levels directly from the DNA of the plants … that’s the slap of realism behind the thunderous announcements.

Trilogene is also on this race :

Now don’t jump on your chair, passing from a successfully R&D demonstration to a large scale industrialization of the process isn’t a piece of cake, specially with polys and even with the nice sequencing tools we have today.

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Sterile and fem cannabis have been out for a while.
You can get a sterile fem plant but you need to mutate it with something like Oryzalin (in weed impede ironically) and then becomes 4 chromosome polyploid i believe then u can cross with a 2 chromosome fem youd get 3 chromosome mule idea.
I guess if everyone does it and someone hermies their polyploid then it can cross with another polyploid but it would have to be matching odd:odd or any even unequl :even i.e 2:4 4:8
All odd:evens would be no match to make seeds.
Like 3:2
Not sure about 3:3 if selfed.
From goolag:
Cannabis is normally diploid, with two sets of chromosomes. But triploidy (3 sets of chromosomes) and even tetraploid (4 sets of chromosomes, 2 female & 2 male) are possible, more so in plants than animals. Triploid cannabis could be created by crossing diploid and tetraploid parents.

Forgot link

I tried it on seed soak. Root got fat af and they grew at snail pace rotted n died lol

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We are not talking about the same constraints at all buddy, but i got you. Now if you have the stellar method to stabilize a poly (in anyway) … just take your millions, Oussama will be fired in the minute and Steeve will kidnap you ^^

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True but someone mentioned it.
Cool i posted the how to.
Science can be more than exciting to some i guess.

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I’m an empirical guy from no where, only sustainable results have my attention.

No explants survived the 150 μM treatment. Survival rates for explants treated with 20 μm oryzalin ranged from 62.5% to 87.5% for strain 1 and 2, respectively

Far better that my 25% survival rate with col/gib. The use of this herbicid was tricky i must admit, even if it’s totally banned as fuck in EU for good reasons ^^

However, oryzalin has greater specificity for plant tubulins (Morejohn et al., 1987) and is considered a more effective and less toxic alternative to colchicine (Petersen et al., 2003; Stanys et al., 2006; Ascough et al., 2008; Dhooghe et al., 2009; Sakhanokho et al., 2009; Viehmannová et al., 2009; Rêgo et al., 2011). Trojak-Goluch and Skomra (2013) found that 1250 μM of colchicine applied to explants was the most effective for polyploidization of hops. Shown here, concentrations in the range of 20 and 40 μM were the most effective for tetraploidization of Cannabis, indicating that oryzalin is effective at over 30 times lower concentration compared to colchicine.

Was worth the ride to read it entirely just for this, even if it complicate a bit the sourcing.

Not really ^^ You should maybe read in details the two procedures involved, for results that are absolutely not insured or even linked to a mass production of “sterile seeds”.

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If you watch the full video interview 1 hour 40 i think it is Breeder Steve talks about the different methods being used to try and achieve their goal of terminator seed in short.

Breeder Steve said by selfing cannabis by the s5 or s7 his getting sterility with only the reversal.

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I brought the seeds and they were leftovers from the Temple Flo seed run I did here. Never met Breeder Steve ever. But I know his shishkaberry and did bring some of that to the gathering. So that’s where the confusion starts.

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I see…
For example.

Where did you get the flo seeds?

From that guy with the shiskaberry.

You mean breeder Steve?

I think that is his name.
:rofl:

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Nice find. Do you recall if they explain the mechanics of why selfing over and over would make them sterile?

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I cant remember to be honest he did go into some detail.

The long video is the one you need to watch.

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https://sci-hub.ru/10.1016/j.indcrop.2013.01.025

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Easiest way to prevent methionine from converting to ethylene is cobalt.

Test any herm you find a hiccup in Acc synthesase phosphorylation.

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In spite of it has been stated that cannabinoids occur in glandular trichomes our investigation detected that the first, male flowers with THC content similar to female flowers and CBD content of 3-fold of female flowers did not have any glandular trichome, and the second cannabinoid content increased even up to 9-fold of untreated plants did not have any effect on leaves glandular trichomes numbers

It would seem THC was increased but trichome production was not.

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So there might be different mechanisms or biochemical pathways for the resin heads? Hmmm yeah that could be why not. Don’t resin heads have their own set of cells in the head’s that can detach from the plant stalk.

I am not sure about the total morphology of the resin stock or head, and I think I need to really look into that and see how how they’re truly formed.

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Found this and will try to dump more info. I’m try not to deviate from this topic, but this is something I found was a difference between males and females.

Three types of glandular trichome have been described on female cannabis, viz bulbous, sessile and capitate stalked. Males have been found to exhibit a fourth type – the antherial glandular trichome, which has only been found on anthers (Fairbairn, 1972).

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Not sure i’m on the subject but trichomes react to abscissique acid, just like fruits. I guess you can find extensive practical infos with passionate hashmakers.

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In plants, dioecy is thought to originate from hermaphroditism through two main pathways: the gynodioecy pathway, in which there is a gynodioecious intermediate (species with both females and hermaphrodites) and the monoecy (or paradioecy) pathway, in which the intermediate is monoecious (species having both female and male flowers on the same plant) [42]. Theory indicates that the gynodioecy pathway should proceed with two successive mutations on two different genes ,amale-sterile mutation producing and a female-sterile mutation producing males [43,44]. No population getting pathway. It is possible that dioecious plants which evolved through the monoecy pathway depend more on epigenetics for sex determination than dioecious plants evolved through the gynodioecy pathway. Indeed, in a monoecious plant, two types of flowers can be produced, either male or female. It seems reasonable to expect that the evolution of separate male and female individuals (dioecy) from monoecious plants would only require a slight change in the regulation of the gene network already in place that controls the spatially separated development of male and female flowers. The findings in persimmons, willows and poplars suggest sex determination in plants can rely on a single gene and often involve epigenetics [45,46]. Interestingly, persimmons and Salicaceae may have followed the monoecy pathway [8,47]. By contrast, two or more sex-determining genes have been foundinAsparagus,date-palm,grapes,kiwi fruits distracting [48–55], and these species may have followed the gynodioecy pathway [8,47]. Therefore, it is possible that the monoecy pathway of dioecy evolution relies more on epigenetics and the gynodioecy pathway on two or more genes, but more species need to be studied to test this hypothesis.

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Y Epigenetics.pdf (604.5 KB)

This is the part where Epistasis was mentioned

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Dr Potter.pdf (3.0 MB)

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