Mushrooms - DIY, spores, etc 🍄 (Part 1)

[7lpdwcaw]

@NonSequitor

Do you take what you consider to be the “champion” fruited from a flush, sterily biopsy on the stem under the cap, and drop in agar?

Thats exactly right. Although I dont do clones with my fruit because I already have enough cultures from the first fruit I cloned and i’ve been isolating and testing the monocultures ever since. All of my fruit (and I think i’ve grown about 6 ounces by now) come from these tiny tissue samples.

@NonSequitor

How do you get the agar culture … and how do you decide what genes from which mushroom go in the agar?

You take a small piece of the agar for the best looking growth and transfer onto another plate and let that grow out. You wont know how well it will perform until you test it by fruiting it which is what I am in the process of doing currently.

@NonSequitor

Thanks when you say transfers you mean you transfer your established line from one agar dish to another? How much agar do you use to inoculate your first quart it grain jar? Thanks

I personally use half pint jars (450ml) as my quart jars I use for my cannabis is too big for my pressure cooker. I use 1 dish for every 2 jars. I cut a grid in the agar and then stab and pick up chunks and drop into the jars, and then shake like hell to get them distributed among the grain.