Thanks for the response @leetdood 
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So tissue culture does not necessarily prevent HLVd… Interesting.
Do they have average number of times ratios for a clean strain?
It’s potentially a lot of work to find the clean base cut again…
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“Tissue Culture for Elimination of Pathogens”
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Yes it is a lot of work and you have to be trained in meristem tissue culture specifically. Thats why hlvd remediation can cost 10k.
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Above all, I remember that an old cut purchased after regeneration under tissue culture is not the ultimate guarantee of the cut having returned to its peak.
As a result, use the term of tissue culture is more for “sales” than certifying purity…
I specify that I am talking about a cut which is not regenerated multiple times with testing each time.
It’s always good to know
Thanks guys 
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Regular tissue culture can clear out fungal infections etc
But its not hlvd remediation
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09/22/2024
Snow Lotus Bx V1+V2 Preservation Project:
Snow Lotus Bx V1
Got their first feeding today.
Snow Lotus Bx V2
Germinated the last 6. Will use the #6 and MMB x MSD as backup pollen so I can create Bc1 of #6 & MMB if frozen pollen fails.
First V2 above ground. I got impatient and dug it up. Watered with a weak microbe and RapidStart solution.
Stonefarm Sour Diesel IBL Preservation Project
Flowering Day:
8 Remaining: 3 Male/ 5 Female
Remaining Males:
The middle has the strongest stem rub.
Males Removed Info:
7/3/10
Lowest smells on stemrub.
Sour Diesel Breeder/Keeper Run
I will prepare a backup in case the stored pollen is not viable. Thinking over options but likely males from my own stock as a back up. MMB/#6/#5 x MSD.
Frank Lem’Mexze (Testing) and clonestock
Was able to move to another locaton. Best single is to replace MMB mother and multi plant pots are for pollinating.
Blue Oyster almost ready to begin. Hopefully I can use similar techniques for the Embryo Culture.
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Hi @SWIM_Yenobi
No news… well there I am sad it’s my youporn currently your culture and no update lol 
.
I hope all is well and have a nice weekend 
See you
O.B
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09/30/24
Stonefarm Sour Diesel IBL Preservation
Flowering Day 30
Removed another male, lowest on stem rub. The last 2 have similar stem rubs. Musty/Ocean/Pine is the smell I’d describe on the males, slightly pungent but a pleasant nose. One has an approximately a 80% stretch and the other closer to 150% after flip. The lanky expression is also much more vigorous. The smaller expression has a small amount of trichome production.
I will allow pollination to continue for around 2 weeks. I will clean everything then begin Flowering Sour Diesel Keepers/Breeders 1 week after in depth Cleaning (Pressure Washer/Bleach). I will raise humidity outside flower tent as well.
Sour Diesel Keeper/Breeder Project
Began flowering prep, took clones of TD#6 and K#5. Took snips from other expressions and stored in fridge.
Top Dawg Sour Diesel BX4 #6 Structure
Karma Sowahh (2023) x MSD Structure
Backcross Backups
Sour Diesel BX4 #6 x MSD & Midtown Madness B x MSD started and entered seedling stage. Even if pollen is successful with frozen pollen I plan to make these with the leftover clones of MMB and TD6.
Will be returning small plants in front to friends location shortly.
Snow Lotus Bx V1 & V2 Preservation Project
Bx V1
Topped and received their first feeding with microbes.
Structure and Leaf Trait Photographs.
Bx V2
11/12 (91.67% Germination Rate)
Watered with Microbes and RapidStart. Watered with PHd water till standing runoff 6.0.
”Franky Lem’Mexze” Testing
Flowering Day 0
Placed outdoor to begin flowering today. About 5” tall average w/ 3rd Node-set minimum. Will only use Light teas and IPM. Minimum synthetics. Outdoors in greenhouse in 10 gallon pots.
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Time to get to work.
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hi @SWIM_Yenobi
make us dream 
See you
O.B
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Hi @SWIM_Yenobi
The Franky Lem’Mexze any information on the variety to create? The smoke test etc. brief information lol.
And how do you keep the stats for the Sour D offspring?
In short, if you have the time, I like to understand the details and where you are going to accentuate things or not, after bx with the mothers in the project or not etc ?
As usual, congratulations on the job, you make me want it💚.
See you
O.B
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I’m working on your reply @Orange.bud. It is going to take me a minute. I finished most of the plant work yesterday and doing journal today. A lot of work.
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No worries brother it’s nice of you to take the time to detail 
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I’m always posing in the background😊
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October, 07, 2024
Stonefarm Sour Diesel IBL Preservation Project:
-Bloom 100% Strength, Microbe Tea, PH~5.8
-Flowering Day 00
*-11 Plant Run, 5:6 (Female:Male)
Removed all males. Ensured to pollinate every possible point before removal. Cleaned tent with a pressure washer and bleach. When I think of Sour Diesel I think Sour Gas. I know there’s a lot of conjecture but these smell like, pungent sour like a cheese but not funky or skunky in back. It’s like musty good weed from Cali back in the day and fuel, a sharp fuel with a dash of rubber (VSCs) That’s a generalization of 3/5 of the females. 1/5 has the same profile but dulled everywhere but the rubber. 1/5 smells like grass, it’s the little mutant one. Really frosty but other than that I’d probably usually remove this one. Cleaning the room after removal of males took all weekend. I sanitized the room and all gear.
Stonefarm Females (5/11):
# # #
Structure Profiles:
Bodhi Snow Lotus Bx V1/V2 Preservation Project
Snow Lotus Bx V1
-Bloom 50% Strength, Microbe Tea, PH~5.5
-8 Plant Run
-Veg Phase
Waiting for the V2 to catch up. Everything will need to be ready for flower in 4-5 weeks. Likely around 3 months from being finished. I have to space things out to allow pollen to lose viability and then build close enough to seed harvest of last project to not have to worry about any cross pollination from next project to last maturing. Running perpetual seed runs is much more difficult than perpetual flower runs imo. The leaf traits are starting to thin out and resemble Bodhis photos more and more on the V1. The V1 is also much more stable by comparison of the V2.
Snow Lotus V1 Structure Profiles:
Generally very short and Squat formation. Tight Node space with the behind leaf trait being incredibly broad the increasing serration and thinning leaf traits. Several have a totem pole like structure and wish to grow only upward when only given minimal topping.
Group Shots(Leaf Traits):
Group Shots (Structure):
Special Notices:
Snow Lotus Bx V2
-RapidStart 25% Strength, Microbe Tea, PH~5.5
-10 Plant Run
-Vegetative Phase
Frank Lem’mexze
-EWC Tea w/ 25% Strength Fish Fertilizer, Microbe Tea, RapidStart 25% Strength, PH~5.5
-Multiplant Bonded to 1 Run, Male Removal Flower Test
-Flowering Day 2 (Not Sexually Mature):
3 Plants. (x2 MMB & x1 Frank L.)
….
1/3 Plants. (Frank Lem’Mexze)
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I’ll finish my journal entry tomorrow.
I sterilized and organized my seed vault. Dropped ambient very low to take these photos. Just project stock:
OG/Kush/Sour Dubb/Longbottom Leaf
My most prized project stock^
Missing In-house Platinum x Platinum Kush Breath x2 (Full packs)
Long flowering varietals
(Missing Moonshine Haze Rare Dankness (Full pack Regs)
I did not pull out my 90’s seeds from Mr.Nice. I also have some 80’s Purple Hindu Kush and Cabo San as well.
The 80’s are from a Military Customer of mine. Basically it’s like 4th generation Herm seeds with some of the previous generations mixed in the stock. The guy doesn’t smoke because he in the military just likes to watch it grow and gives it away. Collects seeds from all the herms. All started with a bagseed supposedly. It would probably be harder to stabilize then the Mexican lineage beans I started with that had less then a 5% expression rate not show intersex.
I’m trying to map these projects but it’s incredibly difficult compared to previous projects.
Sour Dubb Remix Project:
Keeper Hunt SD x Sour Bubble Stock Open Pollination
No need to preserve either but I will only use males of Sour Bubble to give me a bunch of Sour Bubble stock.
Loran’s Longbottom Leaf Remix Project:
It’s gonna be sweet when I finish these then MotoRebel releases an LBL BX1 but hopefully my Interest helps make that happen.
SFV OG BX1 x Jesus OG Kush
Only using males from SFV. NorStar has an entire Jesus OG line now so no need to preserve. I’ll label this LLBL Homage
LLBL Homage x Longbottom Fighter F2
I will call LLBL Homage V2 only using males from Longbottom Fighter.
OG Project
**Preserve Stock in OG/Dubb/LBL Projects: **
Karma White OGS1, Trapstar, MasterKush, and Goji OG F2, SFV OG, Longbottom Fighter.
Amnesia Project
Somas Amnesia Haze/Moonshine Haze x All Amnesia Feminized stock. Most of these are old Attitude EU stock. Likely these are just reversals or S1s of EU Dutch cuts but that’s exactly what I want.
( x- Seed count not pack count)
CBD Seeds-Amnesia Haze (x6) (I think the company no longer exist), Sagmartha-A-1 Haze (x2), Expert Seeds- Amnesia Haze (x6), Nirvana Seeds- Haze #1 (x1 Old stock), Dutch Passion- Outlaw Amnesia (x1) (Discontinued)
Male- The Somas Pack is from 2014 stock. I think Rare Dankness used stock from Somas 05-09’ Stock to hunt his Amnesia Breeder. Moonshine is Also crossed with Nevilles Haze and Old Trainwreck for Nevilles Wreck. I’m sure it helped curb the flowering time of NH. I may just run two cycles with a 2-3 week delay between flowering tents with clones of the Fem Stock.
Haze/Long Flowering Project
(Most Difficult project by far)
Preserve- Outlaw Amnesia, A-1 Haze (Old Attitude stock), Somas Amnesia, Hazelnut Cream, and Mango A5.
Stock I will cross with Mango A5 (Mango Nigerian x A5 Bx, really a fan of the lineage here. Even if I have to hunt it I feel confident in this being a great male selection and Moonshine Haze (Maybe) with for lineage so will forgo Keeper Hunt elimination rounds:
Nevilles Haze, Super Silver Haze, A-1 Haze, Outlaw Amnesia, Somas Amnesia Haze
I have Triple Burger and Franchise Slaughtering Betsy for Preservation as well.
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that malawi gold = fire,
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Your Soloberries should be in that photo for OG too. Goji x Solos (solos is LAPK x Snow Lotus). It’s a nice setup.
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fruit punch terps for days
i didn’t know if there was a difference between hollywood pure kush and LA pure kush
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You keep lining up awesome projects do you also sleep in between the work you have? Thanks for sharing your passion 
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Thank you very much bud. I’m more of a collector to be fair. I’ll be updating my journal today for last weekend entry and ordered the stuff for Seed Embryo Culture. I’m going to use three basic recipes, but I’ll end up sharing which culture media works or hopefully works best. I’ll also try normal sterilized media with and w/o added microbes. I’ll be starting that soon in an attempt to get the 90’s stock germinated. I’ll go finish that post as well.
Hope everyone is well.
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