The Ebner Effect on Cannabis seed

Going back some, every bag of weed in the 70’s was chock full of seed, except thai sticks of course.
Yet that weed gave the high many of us have been searching for ever since.
Makes you wonder how sinsemilla even entered the picture.

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Yes. But pure monecious varieties are maintained by selection, or they mostly revert to diocy.

Below is an excerpt of Ivan Bosca talking about this that I found here: https://www.hempbasics.com/hhusb/hh4bot.htm

"In an interview with the Journal of the International Hemp Association (October 1994), the eminent Prof. Dr. Ivan Bocsa (breeder of Kompolti hemp, which gives the highest yield of fiber in the world) explained that he has bred only dioecious and unisex hybrids because self-pollinated inbreeding of monoecious hemp produces about 20% lower stem yield than dioecious varieties:

"The natural state in which hemp appears was and is dioecious. Monoeciousness is artificial in hemp, and it can only exist with the help of man, and without selection, the dioecious state will return in two or three generations. It is therefore very hard and demanding to keep 90 to 95% monoeciousness during seed multiplications…

“Furthermore, monoeciousness has two large disadvantages. In the first place… we have established that 20-25% of self-pollination takes place in monoecious hemp…”

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no i am not talking about normally monecious plants. and aborted pistils, tho rare, is a thing in cannabis. breeder steve has seen it before he mentioned on the potcast, but only once in his vast experience so female sterility is possible, the mechanism is unknown, and it is extremely rare. i hypothosize it is rare tho becasue we select agauinst the very thing that may cause it which is cross progeny. hermaphrodites pollinated by a true male in this case. we dont ever keep or use herm seeds so we wouldn’t see the outcome, also even if we did use those seeds occassionally there would be no way to determine which seeds were selfed and which came from the external male pollen. also most ppl are just growing females not trying to seed them. add all this up and you can see why it is rare even if the cause is as simple as a true male pollinating a true herm. to take it one more step ant it’s likely not 100% mutation rate when it happens . you may have to run that test with 1000 plants to get one example even using cross progeny. the study i referred to was not regarding monecious plants however so your assumption is incorrect. if you noticed in my original statement i said the study was on turmips i’m pretty sure, and a quick google search woulda told you that turnips are insect pollinated not monecious, so i fear you are replying above as well without gathering all the facts first. the specific technique applied is called cross progeny which i don’t think is possible in a monecious plant, but i am not biologist as i said

**also cannabis is not necessarily dioecious because hemp is monecious. i am pretty sure biologists have no idea if it was di or mono in it’s original form and hemp is still mono so i would disagree a little with your claim there

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Turnip is a Brassica rapa variety. They have perfect flowers and are bisexual. So since they have perfect, not separate, male and female flowers, you are right they are not monecious. So sorry to misapply that term in a way that could include them. Male sterility is used to cross bisexual or monecious plants that would otherwise self fertilize. Monecious plants can be insect pollinated, as in squash.

As far as sterile female flowers, sure. I don’t see why that couldn’t happen by lots of mechanisms, but I don’t know what your point is about it here, so don’t have much to say about it. Other than, plenty of modern strains are the result of natural selfed plants, like most cookies variants. And an interesting side note: Buddha Seeds had an IC mag thread talking about the production of triploid females that were sterile (so you could potentially grow them around pollen and still have sensimilla product).

It is most likely that diocy was the state of the Cannabis lineage during speciation:

https://www.researchgate.net/publication/239480683_Molecular_Phylogenetics_and_character_evolution_of_Cannabaceae

The ready ability of monecious Cannabis plants to self fertilize, combined with the fact that continued selfing of a lineage usually leads to sterility, also support Cannabis as dioecious in its natural state; diocy is likely the primary mechanism for preventing inbreeding. As does the fact that Humulus, the closest extant relative of Cannabis, is also dioecious.

bro you need to step up your reading comprehension a tad, ik we are all stoned here but c’mon, lol. i said NOT SELFED. i am finished with this convo tho since you are not even absorbing the content before replying. enjoy “correcting” everyone else. i mean for god sakes half the shit you said is agreing with me and supporting my statement yet you seem to think you are refuting ot or something. ik the icmag article i was saying basically the same thing. if you listen to breeder steve as i mentioned earlier on thepotcast this was his same experience. he found out by them beeing covered in pollen with no seeds. the piont is nobody knows how to replicate it except in turnips and other plants, and that method is cross progeny which is definitely not the same as selfing

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:laughing::joy::joy::rofl::rofl: Read my post again. I literally said I wasn’t addressing that point at all, as it isn’t relevant to what I was talking about in the first place. I didn’t refute it. Chill.

I finally found the pot cast you were talking about. There is another, non Australian version by the same name on spotify that is unlistenable. It’s an interesting convo so far, but damn 3+ hours. Turns out the mention about aborted stigmas is at about ~3:05. Unfortunately he doesn’t really say anything else.

If you could post a reference about the turnip thing, I am curious. But I’m not sure about ‘cross progeny,’ since nothing useful comes back for searches.

So my “little smoke” revealed to me a gross error I made in the original setup using the EFG to enhance the cloning process.

The exposure to the electrostatic field must have been nullified by the perlite I used in the grow bag.

Perlite is volcanic glass. Glass is used as an electrical insulator.
And that bag was packed tight.
Needless to say, I’ll have to run this experiment again using coco coir as the medium.:laughing:

Unfortunately I don’t have a good candidate for a mom atm.
Anyway, I am taking EFG cloning enhancement out of the fail category and back on the possible list!

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Speaking of clones, the EFG treated clone is showing a root today, naughty girl.

Telltale yellowing on top leaves and, btw, 22 days.

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Update on original test seed.

#2 from 10 yr treated group turned out to be a very smelly boy. Sad.

The next is F from the control group.

Hasn’t quite shown sex yet imo.

The only survivor of the 7 yr seed, treated.

Now things get a bit more interesting. #3 from the treated 10 yr seed is having a strong reaction to my soil mix which is exactly the same as the previous subjects and is showing extremely early flowering.

I can’t recall off the top of my head what causes that shiny look on the leaves, if someone wants to pitch in, please do.

Next, #1 from the treated 10 yr seed suffers the same shiny condition but just starting flower.

Not much to report on the other group.
That’s all for now.

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Just now discovered Mark Burch has purged his vids.
He had several experiments going, one was corn seed, the other chicken eggs.
Pretty much vids of his setups, none had results yet.
I have access to those missing vids, I’ll write him later if he’ll let me repost them.
Today was a LITFA day.
No updates.

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Hey folks, due to some eyestrain, I have a quick update.

Golden Tiger x Blue Dream 1yr seed test survivors.
All are yellowed from not being transplanted on time, 6-8 days late.
Treateds (2) are in the middle. Not much to see here atm.
Repotted in 1gal containers with 4 1/2 spikes of TLO mix.

Inside of my new 2x2x4 foot tent through the cool green tint viewing door. A very nice option.
Inside are the TigerJack female clones. Treated on left, control right.

They are under a 6000-6500 range 50 watt Ledmo floodlight. 24/0
I want these to monster so I can get clones for my bud. Still have a remnant of of bud.

Cool light around $20 I think.
Ledmos are cool cuz the glass cover is held in place by a type of spring clamp, ez on ez off.
It puts out a lot of bluer light and I want no flowering light temps.

Sneak peek of some very early, extremely dense flowering porn for a post hopefully later this weekend.
To those who discounted this lil’ EFG would show any effects, here ya go!

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Back again with the weekend update.

Here is “F” from the 10 yr control group showing her sex. Awful shy for such a big gal.

Here is F in center with 3 on the left and 1 on right.
Without exception, each treated plant is dwarfed by the control specimens of the group.

Here is “1” from the treated 10 yr group.
This one showed no peculiarities with normal flowering.

Here is “3” from the treated 10 yr group.
Just bursting with flowers, sorta strange looking flowers I’d say.
Doing much better in a new pot with a basic sol mix and no nutes.

Here is the treated 7 yr seed on left with “F” on right in size comparison.
This poor gal survived my stupidity in nearly drowning her.

I had to take measures as root aphids have showed up, rice hulls as a physical barrier and mosquito bits(BT) in the soil.

Yellow sticky traps as well.

That’s all for now.

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Just keeping track. Not a fan of scrolling.

#3 has presently shown no unusual development.”

And #1 was the one that had primary leaf deformation and clockwise swirl early on.

Update.
Golden Tiger x Blue Dream 1 yr old seed.

Potted up to 1 gal. late last week.
Plants are slowly recovering from not being repotted on time.
As we have seen before, treated plants are much smaller than the controls.

Treated.

Control.

That’s all for now.

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All plant update.
This was gonna be a long post but the door bell and phone keep interrupting.

Here are three of the Golden Tiger x Blue Dream control plants.
The middle guy is a guy so buh-bye.
(Actually have been seeing a higher than usual number of females this season, kow.)

His name was C3

Moving on, the two treated seed T3 and T4 plus C4.
These three have the same stature, the other two, C1 and C2 are 2X the height.

I’ll probably send C1 and C2 off to my buddy and only keep C4 as a comparison.
You’ll see why a little further down.

T3

T4

Here’s why
These are some leggy ladies.
#1 from the treated, ten yr old seed group. 15"

Standing in at 23" is treated from the 7 yr old group. #1 on right.

#1 flower set,

#3

#3 flower set.

Depending on the potency and repeatability, a short statured, dense flowering sativa could be quite the boon for indoor growers.
Because, as sativa growers know only too well, here is F from the 10 yr control group.
Maybe I’ll rename her “Boo Boo”.

Hopefully this is opening some eyes around here, minds too.

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Clone testing the EFG.

Trying again with some clones, took 3 off of “F” from the ten yr old control group.
Coco coir this time, boiled for 20 mins and rinsed 3 times. Burpee coir runs salty.
One in the EFG:

And two out:

The one on the left is sprinkled and mixed with paramagnetic rock.
The right one is plain.
No hormones.
I didn’t soak these in h2o for 15 min like I usually do and the last two showed wilting initially, the one in the EFG showed none, like my first test.

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Just found out my comments aren’t showing up on youtube.
wtf?

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Sorry for the delay in updates.
Working through a sprained ankle.
Wasn’t able to tend to the plants for several days and when I could my yellow sticky trap was full of fungus gnats. These were my Tiger Jack monster clones and a reveg.
Someone used non sterilized Burpee Starting soil for their flowers.

Here is the aftermath, the reveg and the control clone are showing overwatering stress due to an overnight soaking in soapy water. Incidentally the one in the middle doing well was exposed to the EFG.

The last round of clones, the big one was exposed to the EFG for 24 hrs. Based on the literature @anon4675195 provided upthread.

I have some root porn but was taken by my tablet and are poor quality.
The EFG clone and paramagnetic rock test clones were showing nubs at 7 days.
However the control showed 1/4" root!

Here are residents of my flower tent.
The 7yr old treated exploded with unrestrainable sativa growth and I had to take her out.
C4 from the GTxBD control group turned out male.

From the lower right going counter clockwise:
“F” control
#3 treated
T4 treated
#1 treated
T3 treated
C1 control next to #3
C2 control bottom left

#1, reaching.

Now for the bad news.
Last night my timer failed to turn off my lights, may have happened two other nights as well.

Aaaargh!

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Partial update.
Pic comparison of #3 treated and Control 2.

Close ups of 3 Treated,

Close up of Control 2

To my eye, the seed bracts of 3 Treated are more swollen and the flowers have a strange, fleshy, tentacle-like feel that doesn’t come through in the pics.

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Sorry but probably no updates except this for maybe a week.
Busy time of year. Regular garden seeds need planting.
So just a quick what’s new w/o pics.
#3 is still a dwarf but heavily flowered.
#1 is still in stretch though showing signs of slowing.
Same with Control 1 + 2
F is suffering some malady after I chopped the broken top off,(it had 70-80% amber trichs.:confused:)
T 3+4 are still stretching - other than stature(shorties) no peculiarities.

The 3 clones I took from F had 2 take root, the treated and control and are in 24hr light. The one with paramagnetic rock didn’t make it.

I have to attribute mosquito bits infused water (2 tbp per gallon distilled or rain) having really controlled my gnat troubles. I saw only one this week as I moved my wife’s flower pots around and not a sign in my tents.

Upthread I mentioned a faulty timer issue. Never had one fail before.
This is the one:

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