Meristem cultures can lead to clean cuts, people not doing the work is another thing lol
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If people understood just how much power payment processors had, they would be appauled.
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ZZZZzzzz. The whole idea of what these guys are doing is old news. I had a blue ocean business plan for this back in 2019. Good for them for trying to execute it with a few popular mouthpieces in front of a camera. Honestly, I think it is more important to teach people how to do TC correctly than to keep someone’s for a year and put the work in for cash.
Nodal cultures do not experience somaclonal variation but meristems do.
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From what I understood TC is not a magic wand tthat remove any pathogens. It’s relying on TC growing the plant so fast that some parts will outgrow the pathogen and can be used as a cleaned source. But it’s probably not so easy to achieve, you have to have the right TC recipe for the cultivar.
Kristian adamek (and al) from the uni for Guelph has made some researches on somaclonal variations, including in TC:
Some people have found what they think is more effective way, using flowers:
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Lol just trying to advertise your TC company I see. Charging 2k for a 7 hour course is shameful. @funkyfunk said it perfectly
Anyone claiming they can 100% clean any clone is outright lying. Not to say it cant be done. But sounds like trying to capitalize on peoples ignorance of the process.
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Fingers crossed for an old Sensi Star cut!
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This is pretty ironic because you just advertised it, I didn’t.
The introduction TC course I teach is 1 on 1, two full days, and $2k. If you really want to know how to do TC then come to Sacramento I will teach you how to do it for free. I do this for knowledge transfer, not a cash grab
I am happy to point you to a handful of my peers who all charge $5k+ for one day.
You are mistaken if you think it is impossible to clean pathogens from a plant. My alma mater has a whole commercial program devoted to it. About Foundation Plant Services
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Didn’t know you had a course. I wonder if you have feedbacks from your students (and yourself) about that TC and decontamination topic.
No one says TC can’t remove pathogens, just that it ain’t as easy as sometimes advertised. Top notch facilities with arrays of testing machines in the labs are able to do it, but that seem to require huge efforts even for them sometimes, so I wonder how good it works on that regard for a DIY home TC.
But maybe I’m just under the effect of the good old “Any sufficiently advanced technology is indistinguishable from magic” saying. 
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TC really has many many uses, but it is very feasibly conquered for the home use, " plants from test tubes " is a classic short book thatll set one straight, i mean you could hold 50 cuts in the sammich drawer in your fridge and not worry for months at time lol
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Are ya tryna beat the hops, or something?
The Grateful Head will be selling clones on Breeders Direct Seed Company along with Skunk VA, Staten Island aka Gabby
https://www.breedersdirectseedco.com/index.php?route=product/category&path=86
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So now we know that with the “Gen zero” word, cuts can be sold 1.5 to 3 times the prices. Looking forward feedbacks. Could be cool to have a side by side grow of the same cut.
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Price point may go up. All the clones have sold out in four days.
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It’s marketed like this, but the Gen0 have a real existence in the process sold (regeneration). Technically it’s less sexy and don’t mean at all “gen0 = successfully regenerated, it’s fire, black friday”. It’s a prototype state, a bit like the concept of “patient 0”, but reversed.
Now the service is a true asset for motherplants drastically sick, infected etc … at the moment to assume that it’s a long care service. Not a magic spell.
Just like in “gardenning supermarkets”, all plants you can see are mostly in vitro stuff coming from the same procedure.
Beside the big words, the crude reality ^^
Setting a QC is looking like complicated. So i can’t imagine a full sequencing to certify an unique gen0 on a bazillion of cuts claimed …
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Breeders Direct has em for 300 per. Most of them seem sold out.
Doesn’t need to be stated though, It’s still marketing imo.
Gen0, gen1, gen2, they’re all just a tc’d clone. That’s it. They have tissue cultured clones for sale. ‘gen’ doesn’t even come into it nor mean anything. They got the original cut somehow and that’s for sure not ‘gen0 from seed’, so why try and tack it on now? TC doesn’t automatically mean it’s clean either.
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Is that about the $300/cut release?
We talked with many TC labs and found even the most sophisticated builds in the country were having this problem.
No kidding!
Maybe they should have done that first? 
Or gather the public infos about this for a start
Many losses of small rooted plants that were barely acclimated
Why not test the whole customer XP / end to end viability before release also maybe?
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Man… $300 per cut…
I think I need to get into the clone business instead of flower. 
(kidding)
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It is not easy and goes beyond learning basic tissue culture and micropropagation skills. Most people I teach use tissue culture techniques to incorporate nodal/meristem into an existing nursery program for clean moms, not for pathogen remediation.
You have to test in and test out. Sample tissue to determine whether endophytes are bacterial, fungal, or viral. For bacterial you’d need to know if it were gram - or gram +. You have to use some pretty nasty stuff in your home media that can be pretty hazardous to breathe (although all PGRs are herbicides and hazardous anyway).
Most plants don’t like to grow in this kind of stuff so you gotta do lots of subs and try to “outgrow” your hitchhiker by doing serial tipping. Only then should you try to excise a meristem and this still requires changing blades nearly every cut.
Can it be done, sure? At home, not super likely.
100% of my students take a course and/or pay for extra shooting and rooting protocols, that is it. I don’t sell or share any of my antipathogenic media protocols.
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I don’t understand what the issue is here. Is it shipping or acclimating?
Acclimating TC is not rocket science. It is better NOT to acclimate and let the client do it. Why don’t they just teach people how to acclimate properly?
Heck, Node puts instructions right on their website https://www.nodelabsca.com/resource
So they are telling their customers that they add bacteria and fungus back onto a tissue-cultured plant?
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