The when and where we are still figuring out but I think the ideal time would be at flip date then maybe one a couple weeks later? More testing needed.
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Apparently the xylem transports water and nutrients to the leavesā¦would be my guess
Not sure if you want to stick a needle in it or just overcome the internal pressure or how long
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Iām going to crush and twist the stem at the injection site prior to pumping it with CS. Read earlier in the thread that backpressure will sometimes not allow CS to be plunged into the plantā¦couldnāt remember where injection was supposed to be though. But a good olā stem crushing should make it all work.
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What about shooting it straight into a root?
Thank You @Pedro_Bann I will be flipping on Dec. 24th so I will log everything and report back on my findings.
I am going to do it at least two different ways. Maybe directly into the wood. And in a different branch crush the wood and inject into it. I might also try to inject into a green shoot. Just to try several different methods.
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For some reason they claim that you are not supposed to get the silver on the soil. Not sure why.
Although the roots do absorb the nutrients. So Iām not sure how that would work.
I donāt want to try it on this run because since it is down lower on the plant it will mess up my findings on the other tests.
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Okā¦ Gotta ask since it came up in a different forumā¦ And it just seems odd to me.
Anyone use CS to reverse - then collect that pollen and use it to make feminized crosses of a different mother? I suppose it works, but it seems like a strange way to breed for me,
Other genetic question that came from that - if you reverse a mom and self it. That mothers phenotype is the only result - clone of mother basicallyā¦ Youāre not going to find other phenotypes from S1 seeds am i correct or did i misunderstand something along the way?
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What do you find strange about it? I think that is what a lot of people do, unless I misunderstand something. To me itās definitely less strange than selfing. And S1 is not a clone, itās S1.
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So when the plant DNA splits, only half of it is given from each āparentā. The split is somewhat random, this is why siblings only look similar and not identical. When the DNA is recombined, the new offspring will still have variation.
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Maybe Iām just used to the tropical fish breeding end - using male/female parents. I can see preserving specific plants for further work but i donāt think Iād want to work exclusively from crossing females back against femalesā¦ We know the issues of inbreeding - but when you totally remove the Y chromosome from the breeding process - seems a much faster way to create issues.
Breeding animals is a completely different process from breeding plants. Most common vegetable plants are monoecious, meaning every plant has both male and female parts to the flowers. Most cannabis varieties are dioecious, meaning a separate male and female plant. Production of female flowers is dependent upon ethylene biosynthesis in the plant and all that āreversingā through use of silver ion solutions, most commonly the use of Silver Thiosulfate Solution, does is inhibit ethylene biosynthesis, thus the female plant produces a male sexual expression.
As drug based cannabis phenotypical expressions are most preferred in female plants, a āgynoeciousā breeding program whereby only female plants are used to breed speeds up the process as the only way to tell what a maleās genotype holds is currently through observation of progeny. We know what the phenotypical expressions of both parent plants are going into a āgynoeciousā breeding program, thus taking out the need for observation of the progeny generation to understand the pollen donorās part. It also becomes helpful in legal states where plant numbers are important under the law.
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Oh that i get, but then i go back to fish breeding where usually f4/f5 range I would find myself needing to cross back in both cases to the original parents or outcrossing due to genetic issues.
Having less genetic diversity to start with and pollenating just from females within a line it would seem like youād end up at that point of having issues much sooner. Whereas using even a male sibling would keep a bit more genetic diversity/strength in that line. Like a genetic backstop.
But yeah i can see how just working the females side could be easier. Just wonder where that point of deteriorating return comes in
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The key is, you never start with a single female in the program. You are always using multiple females. You want to pollinate several females with the pollen produced from several females and use observation of the progeny to choose the females for the next round. The difference is, you KNOW what the phenotypes of the females you are choosing will be and donāt have to roll dice on what the phenotypes of the progeny from males will be. If we are breeding for female characteristics, the best way to do so is to eliminate the males completely from the picture since we are able to do so very easily.
Once legalization is national, I expect all of the really big corporations who throw in money to this game will ALL run gynoecious breeding programs because it is faster, easier, and takes out the guess work involved with males.
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@slain On the scales do I need to weigh out 0.01 of a gram of the silver nitrate to add to 10ml of distilled water?
Cheers
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So Itās 100mg or 0.1 of a gram to 10ml and then draw up and inject 100 units or 0.1 of a ml.
I my try a slightly higher amount next mixā¦ maybe 150mg and see if it makes a difference. I hope it works for you mate!
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Alternatively you could select a bunch of males that stand out, clone them to keep a copy in veg and then cross them over a stable ibl, then grow out the progeny to observe which male has the best characteristics, i.e traits you want and how well the male passes on these traitsā¦ and then you can use your male as the recurrent parent to inbreed and backcross. As circumstances would have it I am likely to have a whole bunch of males to select from this year so I might give this a serious crackā¦
Unfortunately as I have learned through experiment that having desirable traits is not always an indication of how well the genetics will be inherited, especially for complex traits were multiple locus/alleles are involvedā¦
I spent a fair amount of time using fems to cross and stabilise, but recently have been using males moreā¦ also sometimes I will use bothā¦ I.e use backcrossing of fems to stabilise a pheno while also inbreeding a separate line of regs and then reintroduce the male and backcrossing to get what I want.
I expect in the not too distant future it will be the use of diploids followed by gene doubling, marker based breeding and crispr that big pharma will be usingā¦ same as most food cropsā¦
ā¦so many ways to skin this cat thoughā¦imho thatās what makes it endlessly interesting 
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This is definitely bookmarked and locked into the future for me - you guys blow me away ā¦fkin love it !!
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@slain
can you give us a heads up on where you sourced your miracle from ? I have gone over both Amazon sites just now with no joy mate , will let my fingers do the walking for a bit
cheers in advance
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eBay mate. I think I paid $40 for 25 gramsā¦ though that memory is a little vague 
I get sodium thiosulphate form eBay as wellā¦ I have been tempted to get some cobalt chloride from there as well, but it comes from Poland and wellā¦ I donāt want to attract any attention:$
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Thanks for replying mate. Do I inject around the 2nd week of flower?
Cheers
G
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