I got some from ebay UK for £5.00 for 2g.
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Great info. Bookmarked and will try in the future.
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OK peeps. Here’s the update. I theory injecting the STS sounds great. But, in practice it seems almost impossible to inject the liquid. The liquid just stops. Then it either squirts out of the hole you just made with the needle or breaks out the other side. I did the best I could we will see what happens.
Here is the test:
- Location 1 - Injected Silver Nitrate into older growth
- Location 2 - Injected Silver Nitrate into young green growth
- Location 3 - Pinched & crushed stem and injected Silver Nitrate into older growth
- Location 4 - Injected Silver Nitrate / Sodium Thiosulfate Anhydrous diluted 50% with distilled water injected into older growth.
- Location 5 - I wrapped tissue around branch and taped it. Then saturated it with Silver Nitrate / Sodium Thiosulfate Anhydrous diluted 50% with distilled water.
Location 5
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Do you have 1 more you you could spray silver nitrate on as if it was colloidal silver?
At this time I don’t want to mix up that much solution. I think #5 is basically the same thing. The only difference is that it holds the solution in place where I want it. I will continue to drench it every 5 days and see what happens. If #5 works it might be a better way since you don’t use so much.
Or it will kill it. 
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Hahaha if it works great without touching a needle then I’m happy. I’ve got the silver and needles, just waiting on a plant… and then a 6’4 260lb guy has to get his gf to inject the plant cause I get pukey just looking at a needle lol
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Update on results with injections?
Thank you for your contribution!
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She’s not doing so well. She’s a girl and she wants to stay that way. I’m still working on the one that I did so I will take some pics and update soon.
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So Injection Location #4 does have some change.
-
Location 1 - Injected Silver Nitrate into older growth - No Change
-
Location 2 - Injected Silver Nitrate into young green growth - No Change
-
Location 3 - Pinched & crushed stem and injected Silver Nitrate into older growth - No Change
- Location 4 - Injected Silver Nitrate / Sodium Thiosulfate Anhydrous diluted 50% with distilled water injected into older growth. - Change
- Location 5 - I wrapped tissue around branch and taped it. Then saturated it with Silver Nitrate / Sodium Thiosulfate Anhydrous diluted 50% with distilled water. - No Change
I re-wet the tissue on #5 with Silver Nitrate / Sodium Thiosulfate Anhydrous diluted 50% with distilled water each time I watered the plant.
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Now my question for you guys is should I inject more into #4 so that it continues to create pollen?
Or should I just see what happens?
I would really like to get some seeds out of one of these plants.
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@ttutone
So basically #4 is the STS solution you would normally spray on the plant or that solution diluted 50% more?
Next question. 100mg/10ml is a 1% solution, so can I just buy a 1% solution already made rather than powder/crystal and mix?
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Location #4 has the exact same solution that you would normally spray on the plant. It is not diluted any further.
Formula:
Powder was mixed as such.
- 100mg silver nitrate to 10ml distilled water
- 250mg Sodium Thiosulfate Anhydrous to 10ml distilled water
Then mix .25ml silver nitrate into .25ml Sodium Thiosulfate Anhydrous then add .5ml of distilled water.
.25ml silver nitrate
.25ml Sodium Thiosulfate Anhydrous
.50ml distilled water
-----Equals------
1ml of mix
I mixed mine from powder that I got off of ebay. I’m not sure if you can buy it pre-made.
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Something like this?
https://www.amazon.com/dp/B07JDCTF6N/?coliid=IJ3FG6NPAW2YQ&colid=1FE000IMU6UGU&psc=1&ref_=lv_ov_lig_dp_it
or
0.1M silver Nitrate Solution (98.3% Distilled Water, 1.7% Silver Nitrate)
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It becomes question on how much you value getting seeds and how much you value research?
IF (and it sounds like this is the case) seeds are high priority… I would actually continue spraying that branch.
Reasoning behind this being to ensure seeds (spraying is the ‘standard’) and now we have only one branch left. Secure seeds, take what was learned here and use it as basis for next round.
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How far down from the top was the injection? It sure worked on that top bud.
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Very good point.
I got to thinking and decided my experiment wasn’t the greatest. A better way would be to take some clones and do a different method on each of them with a control. That way there would be no way the silver could bleed over to a different branch.
Seeds aren’t too much of priority. I can always buy more. 
I think I’m going to shoot branch #4 up one more time.
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About 14" or 36cm from the tip top. And 12 nodes from the top.
Maybe that was part of my problem also. Maybe I need to be closer to the top.
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Interesting… so that’s a curious result given the thiosuphate in the spray principally allows transport of the silver via the leaves lol. still… worth more experimenting with obviously…
I’ll be back on this in a couple of weeks, I’ll set up some more rigourous test regimes myself and see what eventuates.
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Just a quick update.
Location 3 The stem dried out and broke and the bud is dying. The pinched and crushed stem was just too much trama on it.
Location 4 Is looking ok
I hope it drops some pollen.
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Does anyone here with breeding experience think that this will release viable pollen and pollinate itself? It just seems like it’s taking awhile. Although maybe I am just being impatient. It was switched over to 12/12 on 12/24/2019. Any ideas?
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