Apollo11 x f13xApollo11 Seedrun

[antheis]

i got this from icmag:

@chimera

5% bleach in autoclaved, distilled water with a couple of drops of Tween-20 per liter of solution. I sterilize the water in Duran jars (loose lids) in a pressure cooker @ 20 PSI for 20 minutes, and then allow the jars to cool under my flow hood and tighten the lids when the water has cooled. This is probably overkill for most purposes, but it’s a variation of the protocol I use for sterilizing explants for tissue culture, and it seems to work with minimal contamination. You can get away with a drop of dish detergent instead of the Tween, I just have the tween-20 in the lab so I use it. If you are going to be doing a lot, you can order a few ml of Tween-20 on Amazon if you like.

First I rinse the seeds in a tight mesh strainer to remove any leaf debris that might be harboring bacteria or spores. These seeds were a little dirtier than most having been field grown, so you definitely want to wash away any superfluous materials. This can be up to a 5 minute rinse in lukewarm water. Cannabis achenes have a lot of little crevices where spores and bacteria can hide, so a thorough rinse is a wise step. Then I take the seeds and drop them in maybe 100-200 mls of the bleach/Tween-20 solution in an Erlenmeyer flask, but any clean glass vessel will do -the seeds should be fully immersed, maybe 2x the volume of water as seed.

You want to keep stirring and swishing the seeds around gently to ensure the current is washing away any contaminants. After a minute of swishing I remove the bleach and strain the seeds from the liquid and start again. No more than 3 minutes total, and if I see bleaching on the small test batch (always do a test batch to seed how the seeds react), sometimes I’ll only do 2 minutes.

The next part is key. Immediately rinse 3-5 times (for 5 minutes per rinse) in the same sterilized distilled water (baby water / distilled water from Walmart or the local pharmacy is fine), again using a tight mesh strainer, or simply rinse the seeds in a different vessel with the same volume of water, at least 3 times to ensure the bleach is removed. I use a labratory orbita shaker, but you can do it by hand it’s just a little tedious. Once the rinses are completed, soak the seeds in the distilled water as before, for 2-24 hours depending on the age of the seed and the medium you will be growing in.

Sometimes I’ll use agar plates, but most often I’ll just sprout them in paper towels and transfer to a light dirt (like Light Warrior for you Americans) or surface sow on a .peat based planting substrate. You can use whatever medium you prefer, it’s not that big of a deal as long as the seeds remain moist.

HTH, -Chimera

i didn’t use distilled autoclaved water for the solution or the rinse. i just run them in a mesh sieve under the tap for 5 minutes for the rinse.

[ReikoX]

I used that exact method once with some of the old Ancestral Skunk seeds. Anymore, I just soak in chlorinated tap water.

[antheis]

your old ancestral skunk thread on icmag is where it is from i do believe.

[MrMayhem1134]

I would still be interested in giving these a go. Still sounds like a great cross.

[Aglanthia]

I wouldn’t mind having some of these in my back pocket.

[antheis]

I apologize for not being clear.

If anyone wants these low germ rate seeds they need to DM me their info.
simply stating your desire for them on the thread won’t get them in your mailbox.

[antheis]

i have one f2 running that is showing apollo genius dominance.
i topped her a month ago and she’s growing sideways more than up now.

this bed is overrun with roly poly bugs and they are munching all on her lower leaves.

[ReikoX]

@antheis

this bed is overrun with roly poly bugs and they are munching all on her lower leaves.

Might want to scope some lower leaves, looks like some thrips damage to me.

[Rhino_buddy]

@ReikoX

looks like some thrips damage to me

I agree. Got that slug trail, stained glass look. I just got my thrips eradicated a few weeks ago. No signs of more yet, but I’m still acting like they’re there. Dumb pests. I tossed a handful of lady bugs in there and they are still hanging around.

Looks like you can even see some thrips on the lower left of the last photo.

[antheis]

oh snap you guys are right. I just thought it was tiny pieces of rice hulls and q residue on the leaves.
Time to get mix up some ipm.

[Rhino_buddy]

Hate those buggers. Was my first time having them, and at least they aren’t the worst to get rid of.

I’m sure they came from my land. I notice when walking in the understory of our woods, I get bitten by them frequently. Gonna have to be more cautious from now on.

Hope they are gone quickly for you.

[AceHigh]

Looking good