I ran those not long ago. They were really fast to grow!
10 Likes
Are those the JMF? Iām guessing yes because those seem to be a more traditionally shaped mushroom. What were your thoughts on the end result?
āWait, is that those Droids I was looking for?ā Lol
3 Likes
Ok, so last night I decided that the Melmac and JMF were far enough along to inoculate the next round. Next up is Golden Halo (a gold-spore strain from Jamaica) and the mysterious Enigma strain. But when I pulled the syringe out of the bag, I found the oddest thing. It looks to me like the spores germinated in vitro inside the syringe maybe? Or maybe the spores contained some live mycelium due to having to dig around inside the odd shape of the Enigma strain looking for them? Maybe my syringe is just fucked up and contaminated, but it looks everything like mycelium. I guess weāll see because I still inoculated the jars, if itās contamination it will be pretty clear. Plus the same syringe was used to successfully Inoculate an agar plate, so Iām really leaning away from the contam theory. If it was fucked up then the agar plate would show that pretty quickly. That dang Enigma strain sure lives up to itās name.
Edit: this picture is not going to be enough to tell what this is. I realize I said it looks like mycelium, and then the pic looks like a huge booger or a kombucha scobi, but it had created a fully white line along one side all the way to the needle. I had to suck in air in order to clear the syringe. Iām thinking it either made that line as things settled to the bottom or the spores made use of an air bubble on top. If Iām lucky and itās mycelium, then I guess this is my first try using liquid culture.
13 Likes
Enigma is a clone it doesnāt come in spores, only liquid culture or plate. Thatās still very odd lol it looks like a mushroom started to grow in the syringe.
3 Likes
Right. As I was typing that out I started to wonder if there is even such a thing as Enigma spores. Well then I guess itās been a liquid culture this whole time and I did nothing extra to keep it alive. Weāll see how these jars do. They should kick off faster than spores right?
Is that what āIsolated Syringeā means? Iāve been doing this for ages, but have only recently had the means to explore more of the odd terms and techniques. In the past I didnāt do much to preserve my strains, but I already have a nice little collection on agar and want to transfer to slants here soon.
2 Likes
Ya looks like you have had that syringe a while. Did you store in the fridge? The cold will slow germination down but not stop it. Probably not contam if came clean on agar. You should be fine just shake the living hell out of it and put to agar. If itās true enigma that is a live culture strain only as itās a genetic mutation that doesnāt drop spores.
Iām guessing isolated syringe isnāt referring to the syringe but to the variety of mushroom. When you find characteristics you like you can clone and then isolate to stabilize that characteristics. Just a guess google mushroom isolates.
2 Likes
Iām still watching and considering, but a couple more questions if I may -
Do you need to rotate strains (like we do w cannabis) to continue to enjoy new benefits and avoid tolerance?
A single shoebox monotub with a couple flushes seems like it produces a pretty good haul? And then if you need to rotate different strains (is that what you call them?) Youād have to get a few more tubs going, and it seems like you would end up with a shit ton of mushrooms.
Not that thatās necessary a bad problem to have, but Iād have no one to share with and donāt necessarily want to have a big stash in my home for obvious ( I donāt live in Canada) reasons. I know usage is individually dependent, just wasnāt sure if a flush lasts a month or 3 months as a rule?
2 Likes
They are varities not strains. No you do not have to rotate. You can get between 14-28 g dried per flush per shoebox. 2 good flushes then 2 meh flushes. You can grow multiple varities or just one. You can have 1 shoebox or20. Also look into Pf Tek using brown rice flour to make little individual grows. Great for beginners.
7 Likes
I figured out why the penis envy spores werenāt viable. The florida f+ wasnāt either. Same order. Froze somewhere in the shipping process. Probably got left overnight in my mailbox. Note to self. Order earlier like Sept.
5 Likes
Yea freezing kills/drops vitality. A lot of reputable vendors will send the syringes with an ice pack during the Summers and a heat pack during the winters. My 2nd Golden Teacher syringe froze for 2 days because my fiance rearranged the fridge, and put my bin with syringes closer to the back where the cold air comes out. When I went to use it, I saw it was frozen. But still worked out. But general rule from what Iāve read is freezing will kill em
6 Likes
Got 12 more jars in the PC right now, will be doing another 12 tomorrow morning, And if time permits, I have another 12 to do at night or tuesday. Will be doing 36 this run 
Edit: Ok so itās a bit later, and just did agar to grain transfers on the first 12 jars. So weāll see how it turns out!
6 Likes
I love it @ColeLennon
Me thinks printing is fun, i really like how they look on foil.
I love how tall your mushies are growing!
4 Likes
Thank you. I have just started misting the hood and front panel in the morning and let my fogger mist on low for 2 mins every 3 hours and mist the hood and front panel again before lights off and shut the fogger off at night.
3 Likes
Awesome gets addicting doesnāt it? Do you run grain master jars? Just in case thatās where you only noc up a few jars make sure they colonize nice and clean. Then transfer amounts from each nocāed up jar into sterile grain jars. Lose less to contams and when do grain to grain the colonization time is days. Looking good.
@ColeLennon you got a good print game going there!
5 Likes
Yeah, Iāve been looking at grain transfers. I like it.
2 Likes
Are you making multiple prints and discarding the first print? The first hour or two is going to drop the most contamination, then the subsequent ones would be cleaner. Also, try using suspend the cap so itās not touching the foil, hang it with a wire or something.
5 Likes
Yeah, Iāve just figured out the caps put off a lot of moisture. I got a rack I can use.
2 Likes