I understood, and i was explaining the difference, there are a few ways of making liquid cultures.
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Yep, Iām aware. Iāve never done it though. 
You must be reading a ways back in this thread. lol
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the spore tek is the simplest and usually the safest in the sense that there usually is less contamination.
yeah I had a few months hiatus and now IĀ“m catching up hehehe
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So last winter I tried my hand for the first time. Went with corn as my grain and did the whole monotub thing. Multispore syringes I got through reddit. It worked out very well and Iāve managed to supply myself with many years worth of microdoses. I got many large fruits and tons of them.
I had even made myself agar dishes with the plan of making transfers to keep those alive indefinitely. Alas I was away for work so much, those dishes went to shit.
This winter I decided to go at it again. This time I used local harvested rye berries and got liquid culture syringes from the same source. Things havenāt gone nearly as well, bins took a long time to colonize, some basically havenāt. And the fruits Iāve gotten have been much smaller and so far less flushes. Iām not convinced the types I bought were whatās in them either as the āupeā clearly were not upe. Everything looks the exact same between the different bins. But im still getting fruits so im ahead anyway. Coming up on the 2nd flush for a couple of these bins now.
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if you want pure cultures to last, you need to make slantsā¦
and when you do, if you do agar dishes, choose the most vigorous looking mycelium.
in one of your plates there is a clear difference in growth of the mycelium, the more fuzzy one and the more robust looking one, you should isolate the later one and transfer it to a new plate. EC1.1 is a good example of an almost pure culture, the other two show growing patterns that differ.
btw what kind if agar are you using? the media your grow it in should be incorporated in the petri agar medium so the mycelium will recognize it once you colonize the growing medium, fungi have a memory.
hope im making sense.
check RR
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Yeah that ec 1.1 was a transfer of the best leading edge growth from the ec 1.0 plate. I just let things go too long before I got around to checking on them. Thatās alright though, I dont mind starting overā¦I have more than I know what to do with.
Iāll look into slants, though I donāt have a real clean space to be doing mycology work, not do I have much cold space. My plates go into my beer fridge along with my seed collection. This is nothing but an experiment more than a hobby.
When I do get around to more agar work my goal will be to get plates with isolated mycelium, like what I was working towards. My plan was then to do transfers to grain.
I was using some basic agaragar (average gel strength 700mg/2cm) off Amazon with light malt extract.
When you say the media should be incorporated, you mean the grain or the substrate? That sounds like asking for contamination in my world lol.
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Yes like this you are giving a jumpstart to the mycelium, as soon as you transfer it to the final medium it will recognize it and colonize at a higher rate. all you need to do is sterilize everything
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@curiouscat
Iāve got a question and I bet youāll have the answer.
Say one has a liquid culture of penis envy, and they are grown outā¦is there ever a time that they wonāt show the mutation? Like grow looking like a normal cube? Or if you have them, you have them and if you donāt, you donāt.
I just ask because my āupeā looked like normal cubes in the first and second flush before that tub failed. I suspect they were not upe to begin with.
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I sorry dude, didnāt quite understand your questionā¦ lack of coffee and good sleep does this hahah
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Yes, Might be easily achieved by cloning a good looking fruit from second flush but again, a cube is a cube unless itās PE lol
PE has a bad habit of blobbing first flush usually, though with all that locked in genetic craziness of PE Iām not sure youāll ever be truly mutant blob free lol
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Haha sorry.
If you had an LC syringe of upe, they would always look like upe should look? And if they looked like normal cubes you could infer the syringe isnāt upe?
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Right, so I can conclude that my upe are not upe, correct? Absolutely no blobs or anything out of the ordinary compared to other cubes.
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Well upe usually looks like bell capped cubesā¦ Iāve seen some open when most stay closed like pe
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Still waiting on that 3lbs millet grain bag from my kit to colonize. Itās so slow 
I am pretty amused by the fact that this bag of pre-cooked Uncle Benās Brown Rice that I inoculated just for kicks is colonizing way faster than everything else right now, though. (This is after a ābreak and shakeā)
I think I might use it to do grain to grain transfers while Iām waiting for the millet to be ready to spawn 
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Thatās because the Uncle Benās has less volume lol
And nahā¦ Those look like regular old cubes.
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Lol Iāve been had!
One of these days Iām gonna source some legit Genetics, I was really excited for different things until I saw the fruit.
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Mehā¦ Still a trip lol
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if the syringe was obtained from a part of a mushroom other than the spores, ie stem the mushrooms you obtain from this culture should be identical, unless this kind of mutation is caused by any of the environmental factors. otherwise it should be the same as it is a method of cloningā¦
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Just going down this fungi rabbit hole myself, Iāll be trying to catch up on this thread now too! Looks like I lucked out as North Spore is basically up the street from me, so supplies will be easy.
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